Phospholipids in wild-type and LPP3−/−mouse embryonic fibroblasts
| Lipids . | Wild type . | LPP3−/− . |
|---|---|---|
| DAG (pmol/nmol phospholipid) | 15.1±1.7 | 8.3±0.6 |
| MAG (pmol/nmol phospholipid) | 6.6±1.1 | 5.4±2.8 |
| % PA | 0.64 | 0.90 |
| % unknown | 1.08 | 2.15 |
| Extracellular LPA (pmol/nmol of phospholipids/ml) | 1.85±0.07 | 4.7±0.09 |
| Lipids . | Wild type . | LPP3−/− . |
|---|---|---|
| DAG (pmol/nmol phospholipid) | 15.1±1.7 | 8.3±0.6 |
| MAG (pmol/nmol phospholipid) | 6.6±1.1 | 5.4±2.8 |
| % PA | 0.64 | 0.90 |
| % unknown | 1.08 | 2.15 |
| Extracellular LPA (pmol/nmol of phospholipids/ml) | 1.85±0.07 | 4.7±0.09 |
MAG, monoacylglycerol; DAG, diacylglycerol; PA, phosphatidic acid; LPA,lysophosphatidic acid. Values are mean±s.e.m.
There were no significant differences in the major phospholipids phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Percentage PC and PE in wild-type and KO MEFs were 83.2±2.0 (for PC) and 14.4±2(for PE), respectively.