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Table 5.

EGL-30 is required for liquid growth-mediated stimulation of vulva development

Relevant genotype*Growth condition%Muv%Vul§VPC inductionn**Pvalue††
lin-3(n378) NG plates 95 0.5±0.9 41  
lin-3(n378) M9 91 1.1±1.0 44 0.005 versus lin-3(n378) NG plates 
let-23(sy1) NG plates 93 0.7±0.9 80  
let-23(sy1) M9 69 1.7±1.2 80 <0.00001 versus let-23(sy1) NG plates 
let-60(sy95)/+ NG plates 95 0.8±1.0 20  
let-60(sy95)/+ M9 70 2.0±0.9 20 0.0002 versus let-60(sy95dn)/+ NG plates 
egl-30(ad805); let-23(sy1) NG plates 95 0.8±1.0 64  
egl-30(ad805); let-23(sy1) M9 86 0.9±1.1 70 0.6 versus egl-30(ad805); let-23(sy1) NG plates 
sur-1(ku1) NG plates 54 2.5±0.7 25  
egl-30(ad805); sur-1(ku1) NG plates 25 2.8±0.4 20  
let-23(sa62gf) NG plates 90 3.8±0.4 21  
egl-30(ad805); let-23(sa62gf) NG plates 85 4.5±0.8 20  
let-60(n1046gf) NG plates 73 4.0±0.8 22  
egl-30(ad805); let-60(n1046gf) NG plates 60 3.8±0.8 30 0.4 versus let-60(n1046gf) 
lin-15(e1763) NG plates 100 6.0±0.1 20  
egl-30(ad805); lin-15(e1763) NG plates 100 6.0±0.2 20  
pry-1(mu38) NG plates 22 26 2.9±0.5 23  
egl-30(ad805) pry-1(mu38) NG plates 22 3.1±0.4 23 0.2 versus pry-1(mu38) 
let-23(sy1); bar-1(mu63) NG plates 100 0.3±0.5 21 0.91 versus let-23(sy1) on plates 
let-23(sy1); bar-1(mu63) M9 100 0.2±0.3 32 0.43 versus let-23(sy1); bar-1(mu63) on plates 
bar-1(ga80) NG plates 48 2.3±0.8 31  
bar-1(ga80) M9 61 2.3±0.7 31 0.74. vs bar-1(ga980) on plates 
egl-30(ad805) NG plates 3.0±0.0 21  
egl-30(ad805) M9 3.0±0.0 35  
egl-30(tg26) NG plates 3.0±0.0 36  
egl-30(tg26) M9 3.0±0.0 22  
Relevant genotype*Growth condition%Muv%Vul§VPC inductionn**Pvalue††
lin-3(n378) NG plates 95 0.5±0.9 41  
lin-3(n378) M9 91 1.1±1.0 44 0.005 versus lin-3(n378) NG plates 
let-23(sy1) NG plates 93 0.7±0.9 80  
let-23(sy1) M9 69 1.7±1.2 80 <0.00001 versus let-23(sy1) NG plates 
let-60(sy95)/+ NG plates 95 0.8±1.0 20  
let-60(sy95)/+ M9 70 2.0±0.9 20 0.0002 versus let-60(sy95dn)/+ NG plates 
egl-30(ad805); let-23(sy1) NG plates 95 0.8±1.0 64  
egl-30(ad805); let-23(sy1) M9 86 0.9±1.1 70 0.6 versus egl-30(ad805); let-23(sy1) NG plates 
sur-1(ku1) NG plates 54 2.5±0.7 25  
egl-30(ad805); sur-1(ku1) NG plates 25 2.8±0.4 20  
let-23(sa62gf) NG plates 90 3.8±0.4 21  
egl-30(ad805); let-23(sa62gf) NG plates 85 4.5±0.8 20  
let-60(n1046gf) NG plates 73 4.0±0.8 22  
egl-30(ad805); let-60(n1046gf) NG plates 60 3.8±0.8 30 0.4 versus let-60(n1046gf) 
lin-15(e1763) NG plates 100 6.0±0.1 20  
egl-30(ad805); lin-15(e1763) NG plates 100 6.0±0.2 20  
pry-1(mu38) NG plates 22 26 2.9±0.5 23  
egl-30(ad805) pry-1(mu38) NG plates 22 3.1±0.4 23 0.2 versus pry-1(mu38) 
let-23(sy1); bar-1(mu63) NG plates 100 0.3±0.5 21 0.91 versus let-23(sy1) on plates 
let-23(sy1); bar-1(mu63) M9 100 0.2±0.3 32 0.43 versus let-23(sy1); bar-1(mu63) on plates 
bar-1(ga80) NG plates 48 2.3±0.8 31  
bar-1(ga80) M9 61 2.3±0.7 31 0.74. vs bar-1(ga980) on plates 
egl-30(ad805) NG plates 3.0±0.0 21  
egl-30(ad805) M9 3.0±0.0 35  
egl-30(tg26) NG plates 3.0±0.0 36  
egl-30(tg26) M9 3.0±0.0 22  
*

let-60(sy95dn) was linked to unc-24(e138) and was balanced with nT1[let(m435)]. sur-1(ku1) was linked to dpy-17(e164).

Media on which worms were grown.

Multivulva. Percentage of animals that have greater than three VPCs induced.

§

Vulvaless. Percentage of animals that have less than three VPCs induced.

Average number of VPCs induced to vulval fates.

**

Number of animals assayed.

††

P values were calculated for VPC induction using Student's t-test.

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