EGL-30 is required for liquid growth-mediated stimulation of vulva development
| Relevant genotype* . | Growth condition† . | %Muv‡ . | %Vul§ . | VPC induction¶ . | n** . | Pvalue†† . |
|---|---|---|---|---|---|---|
| lin-3(n378) | NG plates | 0 | 95 | 0.5±0.9 | 41 | |
| lin-3(n378) | M9 | 0 | 91 | 1.1±1.0 | 44 | 0.005 versus lin-3(n378) NG plates |
| let-23(sy1) | NG plates | 0 | 93 | 0.7±0.9 | 80 | |
| let-23(sy1) | M9 | 1 | 69 | 1.7±1.2 | 80 | <0.00001 versus let-23(sy1) NG plates |
| let-60(sy95)/+ | NG plates | 0 | 95 | 0.8±1.0 | 20 | |
| let-60(sy95)/+ | M9 | 0 | 70 | 2.0±0.9 | 20 | 0.0002 versus let-60(sy95dn)/+ NG plates |
| egl-30(ad805); let-23(sy1) | NG plates | 0 | 95 | 0.8±1.0 | 64 | |
| egl-30(ad805); let-23(sy1) | M9 | 0 | 86 | 0.9±1.1 | 70 | 0.6 versus egl-30(ad805); let-23(sy1) NG plates |
| sur-1(ku1) | NG plates | 0 | 54 | 2.5±0.7 | 25 | |
| egl-30(ad805); sur-1(ku1) | NG plates | 0 | 25 | 2.8±0.4 | 20 | |
| let-23(sa62gf) | NG plates | 90 | 0 | 3.8±0.4 | 21 | |
| egl-30(ad805); let-23(sa62gf) | NG plates | 85 | 0 | 4.5±0.8 | 20 | |
| let-60(n1046gf) | NG plates | 73 | 0 | 4.0±0.8 | 22 | |
| egl-30(ad805); let-60(n1046gf) | NG plates | 60 | 0 | 3.8±0.8 | 30 | 0.4 versus let-60(n1046gf) |
| lin-15(e1763) | NG plates | 100 | 0 | 6.0±0.1 | 20 | |
| egl-30(ad805); lin-15(e1763) | NG plates | 100 | 0 | 6.0±0.2 | 20 | |
| pry-1(mu38) | NG plates | 22 | 26 | 2.9±0.5 | 23 | |
| egl-30(ad805) pry-1(mu38) | NG plates | 22 | 9 | 3.1±0.4 | 23 | 0.2 versus pry-1(mu38) |
| let-23(sy1); bar-1(mu63) | NG plates | 0 | 100 | 0.3±0.5 | 21 | 0.91 versus let-23(sy1) on plates |
| let-23(sy1); bar-1(mu63) | M9 | 0 | 100 | 0.2±0.3 | 32 | 0.43 versus let-23(sy1); bar-1(mu63) on plates |
| bar-1(ga80) | NG plates | 0 | 48 | 2.3±0.8 | 31 | |
| bar-1(ga80) | M9 | 0 | 61 | 2.3±0.7 | 31 | 0.74. vs bar-1(ga980) on plates |
| egl-30(ad805) | NG plates | 0 | 0 | 3.0±0.0 | 21 | |
| egl-30(ad805) | M9 | 0 | 0 | 3.0±0.0 | 35 | |
| egl-30(tg26) | NG plates | 0 | 0 | 3.0±0.0 | 36 | |
| egl-30(tg26) | M9 | 0 | 0 | 3.0±0.0 | 22 |
| Relevant genotype* . | Growth condition† . | %Muv‡ . | %Vul§ . | VPC induction¶ . | n** . | Pvalue†† . |
|---|---|---|---|---|---|---|
| lin-3(n378) | NG plates | 0 | 95 | 0.5±0.9 | 41 | |
| lin-3(n378) | M9 | 0 | 91 | 1.1±1.0 | 44 | 0.005 versus lin-3(n378) NG plates |
| let-23(sy1) | NG plates | 0 | 93 | 0.7±0.9 | 80 | |
| let-23(sy1) | M9 | 1 | 69 | 1.7±1.2 | 80 | <0.00001 versus let-23(sy1) NG plates |
| let-60(sy95)/+ | NG plates | 0 | 95 | 0.8±1.0 | 20 | |
| let-60(sy95)/+ | M9 | 0 | 70 | 2.0±0.9 | 20 | 0.0002 versus let-60(sy95dn)/+ NG plates |
| egl-30(ad805); let-23(sy1) | NG plates | 0 | 95 | 0.8±1.0 | 64 | |
| egl-30(ad805); let-23(sy1) | M9 | 0 | 86 | 0.9±1.1 | 70 | 0.6 versus egl-30(ad805); let-23(sy1) NG plates |
| sur-1(ku1) | NG plates | 0 | 54 | 2.5±0.7 | 25 | |
| egl-30(ad805); sur-1(ku1) | NG plates | 0 | 25 | 2.8±0.4 | 20 | |
| let-23(sa62gf) | NG plates | 90 | 0 | 3.8±0.4 | 21 | |
| egl-30(ad805); let-23(sa62gf) | NG plates | 85 | 0 | 4.5±0.8 | 20 | |
| let-60(n1046gf) | NG plates | 73 | 0 | 4.0±0.8 | 22 | |
| egl-30(ad805); let-60(n1046gf) | NG plates | 60 | 0 | 3.8±0.8 | 30 | 0.4 versus let-60(n1046gf) |
| lin-15(e1763) | NG plates | 100 | 0 | 6.0±0.1 | 20 | |
| egl-30(ad805); lin-15(e1763) | NG plates | 100 | 0 | 6.0±0.2 | 20 | |
| pry-1(mu38) | NG plates | 22 | 26 | 2.9±0.5 | 23 | |
| egl-30(ad805) pry-1(mu38) | NG plates | 22 | 9 | 3.1±0.4 | 23 | 0.2 versus pry-1(mu38) |
| let-23(sy1); bar-1(mu63) | NG plates | 0 | 100 | 0.3±0.5 | 21 | 0.91 versus let-23(sy1) on plates |
| let-23(sy1); bar-1(mu63) | M9 | 0 | 100 | 0.2±0.3 | 32 | 0.43 versus let-23(sy1); bar-1(mu63) on plates |
| bar-1(ga80) | NG plates | 0 | 48 | 2.3±0.8 | 31 | |
| bar-1(ga80) | M9 | 0 | 61 | 2.3±0.7 | 31 | 0.74. vs bar-1(ga980) on plates |
| egl-30(ad805) | NG plates | 0 | 0 | 3.0±0.0 | 21 | |
| egl-30(ad805) | M9 | 0 | 0 | 3.0±0.0 | 35 | |
| egl-30(tg26) | NG plates | 0 | 0 | 3.0±0.0 | 36 | |
| egl-30(tg26) | M9 | 0 | 0 | 3.0±0.0 | 22 |
let-60(sy95dn) was linked to unc-24(e138) and was balanced with nT1[let(m435)]. sur-1(ku1) was linked to dpy-17(e164).
Media on which worms were grown.
Multivulva. Percentage of animals that have greater than three VPCs induced.
Vulvaless. Percentage of animals that have less than three VPCs induced.
Average number of VPCs induced to vulval fates.
Number of animals assayed.
P values were calculated for VPC induction using Student's t-test.