Relevant genotype^{*}
. | M cell^{†}
. | % Muv^{‡}
. | % Vul^{§}
. | VPC induction^{¶}
. | n^{**}
. | Pvalue^{††}
. |
---|---|---|---|---|---|---|

let-23 (sy1) | + | 0 | 93 | 0.7±0.9 | 80 | |

egl-30 (tg26gf); let-23 (sy1) | + | 7.7 | 65 | 2.6±1.0 | 37 | |

egl-19(n582) | + | 0 | 0 | 3.0±0.0 | 22 | |

egl-30 (tg26gf); let-23 (sy1); egl-19 (n582) | + | 0 | 95 | 0.7±0.8 | 21 | <0.0005 versus egl-30 (tg26gf); let-23 (sy1) |

let-23 (sy1); egl-19 (n2368gf) | + | 0 | 91 | 0.9±1.1 | 23 | 0.27 versus let-23 (sy1) |

egl-30 (tg26gf); let-23 (sy1); egl-19 (n582); syEx465[pmyo-3::egl-19] | + | 4.3 | 65 | 1.9±1.3 | 47 | <0.0005 versus egl-30 (tg26gf); let-23 (sy1); egl-19 (n582) |

egl-30 (tg26gf); let-23 (sy1) | − | 0 | 85 | 1.1±1.2 | 34 | <0.0005 versus egl-30 (tg26gf); let-23 (sy1) 0.09 versus let-23 (sy1) |

sur-1 (ku1) | + | 0 | 29 | 2.8±0.4 | 21 | |

sur-1 (ku1) | − | 0 | 22 | 2.8±0.4 | 9 | 0.74 versus M cell intact sur-1 (ku1) |

Relevant genotype^{*}
. | M cell^{†}
. | % Muv^{‡}
. | % Vul^{§}
. | VPC induction^{¶}
. | n^{**}
. | Pvalue^{††}
. |
---|---|---|---|---|---|---|

let-23 (sy1) | + | 0 | 93 | 0.7±0.9 | 80 | |

egl-30 (tg26gf); let-23 (sy1) | + | 7.7 | 65 | 2.6±1.0 | 37 | |

egl-19(n582) | + | 0 | 0 | 3.0±0.0 | 22 | |

egl-30 (tg26gf); let-23 (sy1); egl-19 (n582) | + | 0 | 95 | 0.7±0.8 | 21 | <0.0005 versus egl-30 (tg26gf); let-23 (sy1) |

let-23 (sy1); egl-19 (n2368gf) | + | 0 | 91 | 0.9±1.1 | 23 | 0.27 versus let-23 (sy1) |

egl-30 (tg26gf); let-23 (sy1); egl-19 (n582); syEx465[pmyo-3::egl-19] | + | 4.3 | 65 | 1.9±1.3 | 47 | <0.0005 versus egl-30 (tg26gf); let-23 (sy1); egl-19 (n582) |

egl-30 (tg26gf); let-23 (sy1) | − | 0 | 85 | 1.1±1.2 | 34 | <0.0005 versus egl-30 (tg26gf); let-23 (sy1) 0.09 versus let-23 (sy1) |

sur-1 (ku1) | + | 0 | 29 | 2.8±0.4 | 21 | |

sur-1 (ku1) | − | 0 | 22 | 2.8±0.4 | 9 | 0.74 versus M cell intact sur-1 (ku1) |

*

In the *egl-30 (tg26gf); let-23 (sy1)* strain, *let-23 (sy1)*was linked to *unc-4 (e120). syEx* denotes a transgene. *syEx465* expresses *egl-19* from the *myo-3*promoter.

†

M cell present or removed by laser ablation during the L1 larval stage.

‡

Multivulva. Percentage of animals that have greater than three VPCs induced.

§

Vulvaless. Percentage of animals that have less than three VPCs induced.

¶

Average number of VPCs induced to vulval fates.

**

Number of animals assayed.

††

*P* values were calculated for VPC induction using Student's *t*-test.

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