Skip to Main Content
Table 3.

EGL-30 requires muscle-expressed EGL-19 to promote vulva development

Relevant genotype*M cell% Muv% Vul§VPC inductionn**Pvalue††
let-23 (sy1) 93 0.7±0.9 80  
egl-30 (tg26gf); let-23 (sy1) 7.7 65 2.6±1.0 37  
egl-19(n582) 3.0±0.0 22  
egl-30 (tg26gf); let-23 (sy1); egl-19 (n582) 95 0.7±0.8 21 <0.0005 versus egl-30 (tg26gf); let-23 (sy1) 
let-23 (sy1); egl-19 (n2368gf) 91 0.9±1.1 23 0.27 versus let-23 (sy1) 
egl-30 (tg26gf); let-23 (sy1); egl-19 (n582); syEx465[pmyo-3::egl-19] 4.3 65 1.9±1.3 47 <0.0005 versus egl-30 (tg26gf); let-23 (sy1); egl-19 (n582) 
egl-30 (tg26gf); let-23 (sy1) − 85 1.1±1.2 34 <0.0005 versus egl-30 (tg26gf); let-23 (sy1) 0.09 versus let-23 (sy1) 
sur-1 (ku1) 29 2.8±0.4 21  
sur-1 (ku1) − 22 2.8±0.4 0.74 versus M cell intact sur-1 (ku1) 
Relevant genotype*M cell% Muv% Vul§VPC inductionn**Pvalue††
let-23 (sy1) 93 0.7±0.9 80  
egl-30 (tg26gf); let-23 (sy1) 7.7 65 2.6±1.0 37  
egl-19(n582) 3.0±0.0 22  
egl-30 (tg26gf); let-23 (sy1); egl-19 (n582) 95 0.7±0.8 21 <0.0005 versus egl-30 (tg26gf); let-23 (sy1) 
let-23 (sy1); egl-19 (n2368gf) 91 0.9±1.1 23 0.27 versus let-23 (sy1) 
egl-30 (tg26gf); let-23 (sy1); egl-19 (n582); syEx465[pmyo-3::egl-19] 4.3 65 1.9±1.3 47 <0.0005 versus egl-30 (tg26gf); let-23 (sy1); egl-19 (n582) 
egl-30 (tg26gf); let-23 (sy1) − 85 1.1±1.2 34 <0.0005 versus egl-30 (tg26gf); let-23 (sy1) 0.09 versus let-23 (sy1) 
sur-1 (ku1) 29 2.8±0.4 21  
sur-1 (ku1) − 22 2.8±0.4 0.74 versus M cell intact sur-1 (ku1) 
*

In the egl-30 (tg26gf); let-23 (sy1) strain, let-23 (sy1)was linked to unc-4 (e120). syEx denotes a transgene. syEx465 expresses egl-19 from the myo-3promoter.

M cell present or removed by laser ablation during the L1 larval stage.

Multivulva. Percentage of animals that have greater than three VPCs induced.

§

Vulvaless. Percentage of animals that have less than three VPCs induced.

Average number of VPCs induced to vulval fates.

**

Number of animals assayed.

††

P values were calculated for VPC induction using Student's t-test.

Close Modal

or Create an Account

Close Modal
Close Modal