Activation of EGL-30 in neurons promotes vulva development
| Relevant genotype* . | Hyperactive† . | %Muv‡ . | %Vul§ . | VPC induction¶ . | n** . | Pvalue†† . |
|---|---|---|---|---|---|---|
| let-23(sy1) | - | 0 | 100 | 0.5±0.9 | 24 | |
| egl-30(tg26gf); let-23(sy1) | -‡‡ | 8 | 65 | 2.6±1.0 | 37 | |
| unc-13(e51) | - | 0 | 0 | 3.0±0.0 | 23 | |
| unc-64(e246) | - | 0 | 0 | 3.0±0.0 | 20 | |
| egl-30(tg26gf) unc-13(e51); let-23(sy1) | - | 3 | 69 | 1.7±1.2 | 35 | 0.002 versus egl-30(tg26gf); let-23(sy1) |
| egl-30(tg26gf); let-23(sy1); unc-64(e246) | - | 6.5 | 72 | 2.1±1.0 | 46 | 0.02 versus egl-30(tg26gf); let-23(sy1) |
| lin-3(n378) | - | 0 | 100 | 0.6±0.8 | 27 | |
| lin-3(n378); Ex [aex-3::egl-30(tg26gf)] | - | 0 | 100 | 0.5±0.8 | 40 | |
| lin-3(n378); Ex [aex-3::egl-30(tg26gf)] | + | 0 | 52 | 2.1±1.1 | 29 | <0.00001 versus lin-3(n378) |
| lin-3(n378); Ex [unc-18::egl-30(tg26gf)] | - | 0 | 95 | 0.7±0.9 | 22 | |
| lin-3(n378); Ex [unc-18::egl-30(tg26gf)] | + | 0 | 42 | 2.4±1.0 | 12 | 0.00005 versus lin-3(n378) |
| lin-3(n378); syEx593 [unc-4::egl-30(tg26gf)] | - | 0 | 73 | 1.7±1.2 | 22 | 0.0008 versus lin-3(n378) |
| lin-3(n378); Ex [lin-31::egl-30(tg26gf)] | ||||||
| F1 generations | - | 0 | 83 | 0.9±1.2 | 40 | 0.3 versus lin-3(n378) |
| Line 1 | - | 0 | 93 | 1.0±1.0 | 27 | 0.2 versus lin-3(n378) |
| Line 2 | - | 0 | 90 | 0.6±1.0 | 21 | 0.9 versus lin-3(n378) |
| Line 3 | - | 0 | 96 | 0.7±1.0 | 25 | 0.8 versus lin-3(n378) |
| Line 4 | - | 0 | 96 | 0.8±1.0 | 25 | 0.4 versus lin-3(n378) |
| Relevant genotype* . | Hyperactive† . | %Muv‡ . | %Vul§ . | VPC induction¶ . | n** . | Pvalue†† . |
|---|---|---|---|---|---|---|
| let-23(sy1) | - | 0 | 100 | 0.5±0.9 | 24 | |
| egl-30(tg26gf); let-23(sy1) | -‡‡ | 8 | 65 | 2.6±1.0 | 37 | |
| unc-13(e51) | - | 0 | 0 | 3.0±0.0 | 23 | |
| unc-64(e246) | - | 0 | 0 | 3.0±0.0 | 20 | |
| egl-30(tg26gf) unc-13(e51); let-23(sy1) | - | 3 | 69 | 1.7±1.2 | 35 | 0.002 versus egl-30(tg26gf); let-23(sy1) |
| egl-30(tg26gf); let-23(sy1); unc-64(e246) | - | 6.5 | 72 | 2.1±1.0 | 46 | 0.02 versus egl-30(tg26gf); let-23(sy1) |
| lin-3(n378) | - | 0 | 100 | 0.6±0.8 | 27 | |
| lin-3(n378); Ex [aex-3::egl-30(tg26gf)] | - | 0 | 100 | 0.5±0.8 | 40 | |
| lin-3(n378); Ex [aex-3::egl-30(tg26gf)] | + | 0 | 52 | 2.1±1.1 | 29 | <0.00001 versus lin-3(n378) |
| lin-3(n378); Ex [unc-18::egl-30(tg26gf)] | - | 0 | 95 | 0.7±0.9 | 22 | |
| lin-3(n378); Ex [unc-18::egl-30(tg26gf)] | + | 0 | 42 | 2.4±1.0 | 12 | 0.00005 versus lin-3(n378) |
| lin-3(n378); syEx593 [unc-4::egl-30(tg26gf)] | - | 0 | 73 | 1.7±1.2 | 22 | 0.0008 versus lin-3(n378) |
| lin-3(n378); Ex [lin-31::egl-30(tg26gf)] | ||||||
| F1 generations | - | 0 | 83 | 0.9±1.2 | 40 | 0.3 versus lin-3(n378) |
| Line 1 | - | 0 | 93 | 1.0±1.0 | 27 | 0.2 versus lin-3(n378) |
| Line 2 | - | 0 | 90 | 0.6±1.0 | 21 | 0.9 versus lin-3(n378) |
| Line 3 | - | 0 | 96 | 0.7±1.0 | 25 | 0.8 versus lin-3(n378) |
| Line 4 | - | 0 | 96 | 0.8±1.0 | 25 | 0.4 versus lin-3(n378) |
In the egl-30(tg26gf); let-23(sy1) strain, let-23(sy1)was linked to unc-4(e120). Ex and syEx denotes a transgene. aex-3::egl-30(tg26gf), unc-18::egl-30(tg26gf), unc-4::egl-30(tg26gf)and lin-31::egl-30(tg26gf) express the egl-30 cDNA with the tg26 mutation from the aex-3, unc-18, unc-4 and lin-31 promoters, respectively. syEx593 is unstable in long-term culture.
Animals displaying faster locomotion and moving with deeper body bends.
Multivulva. Percentage of animals that have greater than three VPCs induced.
Vulvaless. Percentage of animals that have less than three VPCs induced.
Average number of VPCs induced to vulval fates.
Number of animals assayed.
P values were calculated for VPC induction using Student's t-test.
egl-30(tg26gf); let-23(sy1) animals are not hyperactive because of the presence of the unc-4(e120) mutation.