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Table 2.

Activation of EGL-30 in neurons promotes vulva development

Relevant genotype*Hyperactive%Muv%Vul§VPC inductionn**Pvalue††
let-23(sy1) 100 0.5±0.9 24  
egl-30(tg26gf); let-23(sy1) -‡‡ 65 2.6±1.0 37  
unc-13(e51) 3.0±0.0 23  
unc-64(e246) 3.0±0.0 20  
egl-30(tg26gf) unc-13(e51); let-23(sy1) 69 1.7±1.2 35 0.002 versus egl-30(tg26gf); let-23(sy1) 
egl-30(tg26gf); let-23(sy1); unc-64(e246) 6.5 72 2.1±1.0 46 0.02 versus egl-30(tg26gf); let-23(sy1) 
lin-3(n378) 100 0.6±0.8 27  
lin-3(n378); Ex [aex-3::egl-30(tg26gf)] 100 0.5±0.8 40  
lin-3(n378); Ex [aex-3::egl-30(tg26gf)] 52 2.1±1.1 29 <0.00001 versus lin-3(n378) 
lin-3(n378); Ex [unc-18::egl-30(tg26gf)] 95 0.7±0.9 22  
lin-3(n378); Ex [unc-18::egl-30(tg26gf)] 42 2.4±1.0 12 0.00005 versus lin-3(n378) 
lin-3(n378); syEx593 [unc-4::egl-30(tg26gf)] 73 1.7±1.2 22 0.0008 versus lin-3(n378) 
lin-3(n378); Ex [lin-31::egl-30(tg26gf)]       
F1 generations 83 0.9±1.2 40 0.3 versus lin-3(n378) 
Line 1 93 1.0±1.0 27 0.2 versus lin-3(n378) 
Line 2 90 0.6±1.0 21 0.9 versus lin-3(n378) 
Line 3 96 0.7±1.0 25 0.8 versus lin-3(n378) 
Line 4 96 0.8±1.0 25 0.4 versus lin-3(n378) 
Relevant genotype*Hyperactive%Muv%Vul§VPC inductionn**Pvalue††
let-23(sy1) 100 0.5±0.9 24  
egl-30(tg26gf); let-23(sy1) -‡‡ 65 2.6±1.0 37  
unc-13(e51) 3.0±0.0 23  
unc-64(e246) 3.0±0.0 20  
egl-30(tg26gf) unc-13(e51); let-23(sy1) 69 1.7±1.2 35 0.002 versus egl-30(tg26gf); let-23(sy1) 
egl-30(tg26gf); let-23(sy1); unc-64(e246) 6.5 72 2.1±1.0 46 0.02 versus egl-30(tg26gf); let-23(sy1) 
lin-3(n378) 100 0.6±0.8 27  
lin-3(n378); Ex [aex-3::egl-30(tg26gf)] 100 0.5±0.8 40  
lin-3(n378); Ex [aex-3::egl-30(tg26gf)] 52 2.1±1.1 29 <0.00001 versus lin-3(n378) 
lin-3(n378); Ex [unc-18::egl-30(tg26gf)] 95 0.7±0.9 22  
lin-3(n378); Ex [unc-18::egl-30(tg26gf)] 42 2.4±1.0 12 0.00005 versus lin-3(n378) 
lin-3(n378); syEx593 [unc-4::egl-30(tg26gf)] 73 1.7±1.2 22 0.0008 versus lin-3(n378) 
lin-3(n378); Ex [lin-31::egl-30(tg26gf)]       
F1 generations 83 0.9±1.2 40 0.3 versus lin-3(n378) 
Line 1 93 1.0±1.0 27 0.2 versus lin-3(n378) 
Line 2 90 0.6±1.0 21 0.9 versus lin-3(n378) 
Line 3 96 0.7±1.0 25 0.8 versus lin-3(n378) 
Line 4 96 0.8±1.0 25 0.4 versus lin-3(n378) 
*

In the egl-30(tg26gf); let-23(sy1) strain, let-23(sy1)was linked to unc-4(e120). Ex and syEx denotes a transgene. aex-3::egl-30(tg26gf), unc-18::egl-30(tg26gf), unc-4::egl-30(tg26gf)and lin-31::egl-30(tg26gf) express the egl-30 cDNA with the tg26 mutation from the aex-3, unc-18, unc-4 and lin-31 promoters, respectively. syEx593 is unstable in long-term culture.

Animals displaying faster locomotion and moving with deeper body bends.

Multivulva. Percentage of animals that have greater than three VPCs induced.

§

Vulvaless. Percentage of animals that have less than three VPCs induced.

Average number of VPCs induced to vulval fates.

**

Number of animals assayed.

††

P values were calculated for VPC induction using Student's t-test.

‡‡

egl-30(tg26gf); let-23(sy1) animals are not hyperactive because of the presence of the unc-4(e120) mutation.

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