Skip to Main Content
Table 4.

EGL-30 acts downstream or parallel to LET-60 and is dependent on wild-type BAR-1 activity

Relevant genotype*% Muv% VulVPC induction§nPvalue**
let-60 (sy95dn)/+ 79 0.6±0.8 21  
egl-30 (tg26gf); let-60 (sy95dn)/+ 35 2.5±0.9 20 <0.0005 versus let-60 (sy95dn)/+ 
let-23(sy1); lin-3 (n378) 100 0.0±0.0 20  
egl-30 (tg26gf); let-23 (sy1); lin-3 (n378) 100 0.2±0.6 20 0.16 versus let-23 (sy1); lin-3 (n378) 
let-23 (sa62gf) 98 4.3±0.6 41  
let-23 (sa62gf); let-60 (sy95dn)/+ 95 0.5±0.9 20 <0.0005 versus egl-30 (tg26gf); let-60 (sy95dn)/+ 
let-23 (sa62gf)/+; syIs 1/+ 100 5.1±0.7 21 <0.0005 versus egl-30 (tg26gf) 
let-23 (sa62gf)/+ ; let-60 (sy95dn)/+ ; syIs1/+ 100 1.3±0.8 24 <0.0005 versus egl-30 (tg26gf); let-60 (sy95dn)/+ 
bar-1 (ga80) 48 2.3±0.9 31  
egl-30 (tg26gf); bar-1 (ga80) 41 2.5±0.7 22 0.3 versus bar-1 (ga80) 
let-23 (sy1) 100 0.3±0.4 24  
bar-1 (mu63) 3.0±0.0 20  
let-23 (sy1); bar-1 (mu63) 100 0.3±0.5 21 0.91 versus let-23 (sy1) on plates 
egl-30 (tg26gf); let-23 (sy1) 16 48 2.4±1.1 44 <0.000001 versus let-23 (sy1) 
egl-30 (tg26gf); let-23 (sy1); bar-1 (mu63) 95 1.1±1.0 21 0.00004 versus egl-30 (tg26gf); let-23 (sy1) 
Relevant genotype*% Muv% VulVPC induction§nPvalue**
let-60 (sy95dn)/+ 79 0.6±0.8 21  
egl-30 (tg26gf); let-60 (sy95dn)/+ 35 2.5±0.9 20 <0.0005 versus let-60 (sy95dn)/+ 
let-23(sy1); lin-3 (n378) 100 0.0±0.0 20  
egl-30 (tg26gf); let-23 (sy1); lin-3 (n378) 100 0.2±0.6 20 0.16 versus let-23 (sy1); lin-3 (n378) 
let-23 (sa62gf) 98 4.3±0.6 41  
let-23 (sa62gf); let-60 (sy95dn)/+ 95 0.5±0.9 20 <0.0005 versus egl-30 (tg26gf); let-60 (sy95dn)/+ 
let-23 (sa62gf)/+; syIs 1/+ 100 5.1±0.7 21 <0.0005 versus egl-30 (tg26gf) 
let-23 (sa62gf)/+ ; let-60 (sy95dn)/+ ; syIs1/+ 100 1.3±0.8 24 <0.0005 versus egl-30 (tg26gf); let-60 (sy95dn)/+ 
bar-1 (ga80) 48 2.3±0.9 31  
egl-30 (tg26gf); bar-1 (ga80) 41 2.5±0.7 22 0.3 versus bar-1 (ga80) 
let-23 (sy1) 100 0.3±0.4 24  
bar-1 (mu63) 3.0±0.0 20  
let-23 (sy1); bar-1 (mu63) 100 0.3±0.5 21 0.91 versus let-23 (sy1) on plates 
egl-30 (tg26gf); let-23 (sy1) 16 48 2.4±1.1 44 <0.000001 versus let-23 (sy1) 
egl-30 (tg26gf); let-23 (sy1); bar-1 (mu63) 95 1.1±1.0 21 0.00004 versus egl-30 (tg26gf); let-23 (sy1) 
*

let-60 (sy95dn) strains carried unc-24 (e138) and were balanced with nT1 [let (m435)]. let-23 (sa62gf) was linked to unc-4 (e120). let-23 (sy1) was linked to unc-4 (e120) in let-23 (sy1); lin-3 (n378) and egl-30 (tg26gf); let-23 (sy1);lin-3 (n378) strains. syIs 1 is an integrated transgene that contains multiple copies of the lin-3 gene.

Multivulva. Percentage of animals that have greater than three VPCs induced.

Vulvaless. Percentage of animals that have less than three VPCs induced.

§

Average number of VPCs induced to vulval fates.

Number of animals assayed.

**

P values were calculated for VPC induction using Student's t-test.

Close Modal

or Create an Account

Close Modal
Close Modal