EGL-30 acts downstream or parallel to LET-60 and is dependent on wild-type BAR-1 activity
| Relevant genotype* . | % Muv† . | % Vul‡ . | VPC induction§ . | n¶ . | Pvalue** . |
|---|---|---|---|---|---|
| let-60 (sy95dn)/+ | 0 | 79 | 0.6±0.8 | 21 | |
| egl-30 (tg26gf); let-60 (sy95dn)/+ | 0 | 35 | 2.5±0.9 | 20 | <0.0005 versus let-60 (sy95dn)/+ |
| let-23(sy1); lin-3 (n378) | 0 | 100 | 0.0±0.0 | 20 | |
| egl-30 (tg26gf); let-23 (sy1); lin-3 (n378) | 0 | 100 | 0.2±0.6 | 20 | 0.16 versus let-23 (sy1); lin-3 (n378) |
| let-23 (sa62gf) | 98 | 0 | 4.3±0.6 | 41 | |
| let-23 (sa62gf); let-60 (sy95dn)/+ | 0 | 95 | 0.5±0.9 | 20 | <0.0005 versus egl-30 (tg26gf); let-60 (sy95dn)/+ |
| let-23 (sa62gf)/+; syIs 1/+ | 100 | 0 | 5.1±0.7 | 21 | <0.0005 versus egl-30 (tg26gf) |
| let-23 (sa62gf)/+ ; let-60 (sy95dn)/+ ; syIs1/+ | 0 | 100 | 1.3±0.8 | 24 | <0.0005 versus egl-30 (tg26gf); let-60 (sy95dn)/+ |
| bar-1 (ga80) | 0 | 48 | 2.3±0.9 | 31 | |
| egl-30 (tg26gf); bar-1 (ga80) | 0 | 41 | 2.5±0.7 | 22 | 0.3 versus bar-1 (ga80) |
| let-23 (sy1) | 0 | 100 | 0.3±0.4 | 24 | |
| bar-1 (mu63) | 0 | 0 | 3.0±0.0 | 20 | |
| let-23 (sy1); bar-1 (mu63) | 0 | 100 | 0.3±0.5 | 21 | 0.91 versus let-23 (sy1) on plates |
| egl-30 (tg26gf); let-23 (sy1) | 16 | 48 | 2.4±1.1 | 44 | <0.000001 versus let-23 (sy1) |
| egl-30 (tg26gf); let-23 (sy1); bar-1 (mu63) | 0 | 95 | 1.1±1.0 | 21 | 0.00004 versus egl-30 (tg26gf); let-23 (sy1) |
| Relevant genotype* . | % Muv† . | % Vul‡ . | VPC induction§ . | n¶ . | Pvalue** . |
|---|---|---|---|---|---|
| let-60 (sy95dn)/+ | 0 | 79 | 0.6±0.8 | 21 | |
| egl-30 (tg26gf); let-60 (sy95dn)/+ | 0 | 35 | 2.5±0.9 | 20 | <0.0005 versus let-60 (sy95dn)/+ |
| let-23(sy1); lin-3 (n378) | 0 | 100 | 0.0±0.0 | 20 | |
| egl-30 (tg26gf); let-23 (sy1); lin-3 (n378) | 0 | 100 | 0.2±0.6 | 20 | 0.16 versus let-23 (sy1); lin-3 (n378) |
| let-23 (sa62gf) | 98 | 0 | 4.3±0.6 | 41 | |
| let-23 (sa62gf); let-60 (sy95dn)/+ | 0 | 95 | 0.5±0.9 | 20 | <0.0005 versus egl-30 (tg26gf); let-60 (sy95dn)/+ |
| let-23 (sa62gf)/+; syIs 1/+ | 100 | 0 | 5.1±0.7 | 21 | <0.0005 versus egl-30 (tg26gf) |
| let-23 (sa62gf)/+ ; let-60 (sy95dn)/+ ; syIs1/+ | 0 | 100 | 1.3±0.8 | 24 | <0.0005 versus egl-30 (tg26gf); let-60 (sy95dn)/+ |
| bar-1 (ga80) | 0 | 48 | 2.3±0.9 | 31 | |
| egl-30 (tg26gf); bar-1 (ga80) | 0 | 41 | 2.5±0.7 | 22 | 0.3 versus bar-1 (ga80) |
| let-23 (sy1) | 0 | 100 | 0.3±0.4 | 24 | |
| bar-1 (mu63) | 0 | 0 | 3.0±0.0 | 20 | |
| let-23 (sy1); bar-1 (mu63) | 0 | 100 | 0.3±0.5 | 21 | 0.91 versus let-23 (sy1) on plates |
| egl-30 (tg26gf); let-23 (sy1) | 16 | 48 | 2.4±1.1 | 44 | <0.000001 versus let-23 (sy1) |
| egl-30 (tg26gf); let-23 (sy1); bar-1 (mu63) | 0 | 95 | 1.1±1.0 | 21 | 0.00004 versus egl-30 (tg26gf); let-23 (sy1) |
let-60 (sy95dn) strains carried unc-24 (e138) and were balanced with nT1 [let (m435)]. let-23 (sa62gf) was linked to unc-4 (e120). let-23 (sy1) was linked to unc-4 (e120) in let-23 (sy1); lin-3 (n378) and egl-30 (tg26gf); let-23 (sy1);lin-3 (n378) strains. syIs 1 is an integrated transgene that contains multiple copies of the lin-3 gene.
Multivulva. Percentage of animals that have greater than three VPCs induced.
Vulvaless. Percentage of animals that have less than three VPCs induced.
Average number of VPCs induced to vulval fates.
Number of animals assayed.
P values were calculated for VPC induction using Student's t-test.