Activation of EGL-30 promotes vulva development
| Relevant genotype* . | %Muv† . | %Vul‡ . | VPC induction§ . | n¶ . | Pvalue** . |
|---|---|---|---|---|---|
| Wild type | 0 | 0 | 3.0±0.0 | 31 | |
| egl-30(tg26gf) | 0 | 0 | 3.0±0.0 | 20 | |
| lin-3(n378) | 0 | 96 | 0.7±1.0 | 51 | |
| egl-30(tg26gf); lin-3(n378) | 0 | 35 | 2.8±0.7 | 20 | <0.0005 versus lin-3(n378) |
| let-23(sy1) | 0 | 100 | 0.5±0.9 | 24 | |
| egl-30(tg26gf); let-23(sy1) | 7.7 | 65 | 2.6±1.0 | 37 | <0.0005 versus let-23(sy1) |
| phm-2(ad538); let-23(sy1) | 0 | 100 | 0.1±0.4 | 20 | <0.0005 versus egl-30(tg26gf); let-23(sy1) |
| goa-1(n363); let-23(sy1) | 0 | 100 | 0.4±0.9 | 24 | <0.0005 versus egl-30(tg26gf); let-23(sy1) |
| egl-30(ad805); let-23(sy1) | 0 | 94 | 0.6±1.0 | 52 | 0.65 versus let-23(sy1) |
| egl-30(ad805); let-23(sy1); syEx532 [egl-30(+)XS] | 3.6 | 71 | 1.9±1.2 | 56 | 0.004 versus egl-30(ad805); let-23(sy1) |
| Relevant genotype* . | %Muv† . | %Vul‡ . | VPC induction§ . | n¶ . | Pvalue** . |
|---|---|---|---|---|---|
| Wild type | 0 | 0 | 3.0±0.0 | 31 | |
| egl-30(tg26gf) | 0 | 0 | 3.0±0.0 | 20 | |
| lin-3(n378) | 0 | 96 | 0.7±1.0 | 51 | |
| egl-30(tg26gf); lin-3(n378) | 0 | 35 | 2.8±0.7 | 20 | <0.0005 versus lin-3(n378) |
| let-23(sy1) | 0 | 100 | 0.5±0.9 | 24 | |
| egl-30(tg26gf); let-23(sy1) | 7.7 | 65 | 2.6±1.0 | 37 | <0.0005 versus let-23(sy1) |
| phm-2(ad538); let-23(sy1) | 0 | 100 | 0.1±0.4 | 20 | <0.0005 versus egl-30(tg26gf); let-23(sy1) |
| goa-1(n363); let-23(sy1) | 0 | 100 | 0.4±0.9 | 24 | <0.0005 versus egl-30(tg26gf); let-23(sy1) |
| egl-30(ad805); let-23(sy1) | 0 | 94 | 0.6±1.0 | 52 | 0.65 versus let-23(sy1) |
| egl-30(ad805); let-23(sy1); syEx532 [egl-30(+)XS] | 3.6 | 71 | 1.9±1.2 | 56 | 0.004 versus egl-30(ad805); let-23(sy1) |
In the egl-30(tg26gf); let-23(sy1) strain, let-23(sy1)was linked to unc-4(e120). syEx denotes a transgene. syEx532contains the wild-type egl-30 gene.
Multivulva. Percentage of animals that have greater than three VPCs induced.
Vulvaless. Percentage of animals that have less than three VPCs induced.
Average number of VPCs induced to vulval fates.
Number of animals assayed.
P values were calculated for VPC induction using Student's t-test.