Isolated blastomeres . | n . | Endoderm (ALP) . | Muscle (myosin) . | Muscle (AchE) . |
---|---|---|---|---|
B4.1 | 217 | 6% (25%) | 91% (38%) | - |
B4.1 | 217 | - | - | 92% (38%) |
A4.1 | 105 | 31% (38%) | 0% (0%)* | - |
Isolated blastomeres . | n . | Endoderm (ALP) . | Muscle (myosin) . | Muscle (AchE) . |
---|---|---|---|---|
B4.1 | 217 | 6% (25%) | 91% (38%) | - |
B4.1 | 217 | - | - | 92% (38%) |
A4.1 | 105 | 31% (38%) | 0% (0%)* | - |
Isolated blastomeres were dissociated three times at the 16-, 32- and 64-cell stages and then cultured as partial embryos. ALP and myosin were double stained and simultaneously detected in the same specimens. Expectations from the cell lineage tree (Fig. 1)are indicated in parentheses. n, number of partial embryos examined.
A4.1 blastomeres give rise to secondary muscle cells, but secondary- muscle formation requires cell interactions. The cells are not formed from isolated A4.1 blastomeres