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Table 4.

UNC-71 functions cell non-autonomously in D neuron axon guidance

A
Rescuing activity of Max phenotypes*
Tissue and cell typesTransgene promoter+++ (0-10%)++ (10-20%)+ (20-40%)No rescue (>40%)
 Hypodermal cells, neurons and excretory canal cells Punc-115 
 Hypodermal cells Pe15*2 
 Neurons Punc-119 
 Neurons Punc-33 
 Neurons PF25B3.3 
 Interneurons Pglr-1 
 D neurons Punc-25 
 D neurons Punc-30 
 Body wall muscles Pmyo-3 
A
Rescuing activity of Max phenotypes*
Tissue and cell typesTransgene promoter+++ (0-10%)++ (10-20%)+ (20-40%)No rescue (>40%)
 Hypodermal cells, neurons and excretory canal cells Punc-115 
 Hypodermal cells Pe15*2 
 Neurons Punc-119 
 Neurons Punc-33 
 Neurons PF25B3.3 
 Interneurons Pglr-1 
 D neurons Punc-25 
 D neurons Punc-30 
 Body wall muscles Pmyo-3 
B
Rescuing activity of Max phenotypes*
Constructs+++ (0-10%)++ (10-20%)+ (20-40%)No rescue (>40%)
 Punc-115-UNC-71 
 Punc-115-UNC-71::GFP 
 Pe15*2-UNC-71 
 Pe15*2-UNC-71::GFP 
 Punc-115-UNC-71(M-
 Punc-115-UNC-71(ECD) 
 Punc-71-UNC-71 10 
B
Rescuing activity of Max phenotypes*
Constructs+++ (0-10%)++ (10-20%)+ (20-40%)No rescue (>40%)
 Punc-115-UNC-71 
 Punc-115-UNC-71::GFP 
 Pe15*2-UNC-71 
 Pe15*2-UNC-71::GFP 
 Punc-115-UNC-71(M-
 Punc-115-UNC-71(ECD) 
 Punc-71-UNC-71 10 

(A) Rescue of unc-71(ju156) D neuron axonal defects by tissue specific promoters driving the unc-71 minigene. unc-71(ju156) animals display 48±8% axon guidance defects,scored as `no rescue'.

(B) Functional analysis of UNC-71. UNC-71(M-), metalloprotease domain deletion construct; UNC-71(ECD), extracellular domains of UNC-71 only. Twenty to 50 animals were scored for each transgenic line, and the average percentage was presented.

*

Degree of rescue is represented by % axon dorsal guidance defects. The dorsal guidance defects of the D neuron axons, visualized by juIs76,were quantified by counting the number of commissures from DD2-6and VD1,3-13 that did not reach the dorsal cord within a single animal, which was then divided by 17 to convert to the percentage of defects

DNAs were injected at 20 ng/μl and 100 ng/μl, and the results were similar. Only the results from the 20 ng/μl DNA injection are presented

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