UNC-71 functions cell non-autonomously in D neuron axon guidance
| A . | . | . | . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| . | . | . | Rescuing activity of Max phenotypes* . | . | . | . | |||
| . | Tissue and cell types . | Transgene promoter . | +++ (0-10%) . | ++ (10-20%) . | + (20-40%) . | No rescue (>40%) . | |||
| Hypodermal cells, neurons and excretory canal cells | Punc-115 | 3 | 0 | 2 | 0 | ||||
| Hypodermal cells | Pe15*2 | 0 | 0 | 4 | 1 | ||||
| Neurons | Punc-119† | 0 | 0 | 3 | 2 | ||||
| Neurons | Punc-33 | 0 | 2 | 3 | 1 | ||||
| Neurons | PF25B3.3 | 0 | 1 | 2 | 2 | ||||
| Interneurons | Pglr-1 | 0 | 0 | 2 | 6 | ||||
| D neurons | Punc-25† | 0 | 0 | 0 | 9 | ||||
| D neurons | Punc-30 | 0 | 0 | 0 | 3 | ||||
| Body wall muscles | Pmyo-3 | 0 | 0 | 0 | 5 | ||||
| A . | . | . | . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| . | . | . | Rescuing activity of Max phenotypes* . | . | . | . | |||
| . | Tissue and cell types . | Transgene promoter . | +++ (0-10%) . | ++ (10-20%) . | + (20-40%) . | No rescue (>40%) . | |||
| Hypodermal cells, neurons and excretory canal cells | Punc-115 | 3 | 0 | 2 | 0 | ||||
| Hypodermal cells | Pe15*2 | 0 | 0 | 4 | 1 | ||||
| Neurons | Punc-119† | 0 | 0 | 3 | 2 | ||||
| Neurons | Punc-33 | 0 | 2 | 3 | 1 | ||||
| Neurons | PF25B3.3 | 0 | 1 | 2 | 2 | ||||
| Interneurons | Pglr-1 | 0 | 0 | 2 | 6 | ||||
| D neurons | Punc-25† | 0 | 0 | 0 | 9 | ||||
| D neurons | Punc-30 | 0 | 0 | 0 | 3 | ||||
| Body wall muscles | Pmyo-3 | 0 | 0 | 0 | 5 | ||||
| B . | . | . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| . | . | Rescuing activity of Max phenotypes* . | . | . | . | |||
| . | Constructs . | +++ (0-10%) . | ++ (10-20%) . | + (20-40%) . | No rescue (>40%) . | |||
| Punc-115-UNC-71 | 3 | 0 | 2 | 0 | ||||
| Punc-115-UNC-71::GFP | 1 | 3 | 0 | 0 | ||||
| Pe15*2-UNC-71 | 0 | 0 | 4 | 1 | ||||
| Pe15*2-UNC-71::GFP | 0 | 1 | 3 | 0 | ||||
| Punc-115-UNC-71(M-) | 0 | 0 | 0 | 6 | ||||
| Punc-115-UNC-71(ECD)† | 0 | 0 | 0 | 4 | ||||
| Punc-71-UNC-71† | 5 | 2 | 2 | 10 | ||||
| B . | . | . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| . | . | Rescuing activity of Max phenotypes* . | . | . | . | |||
| . | Constructs . | +++ (0-10%) . | ++ (10-20%) . | + (20-40%) . | No rescue (>40%) . | |||
| Punc-115-UNC-71 | 3 | 0 | 2 | 0 | ||||
| Punc-115-UNC-71::GFP | 1 | 3 | 0 | 0 | ||||
| Pe15*2-UNC-71 | 0 | 0 | 4 | 1 | ||||
| Pe15*2-UNC-71::GFP | 0 | 1 | 3 | 0 | ||||
| Punc-115-UNC-71(M-) | 0 | 0 | 0 | 6 | ||||
| Punc-115-UNC-71(ECD)† | 0 | 0 | 0 | 4 | ||||
| Punc-71-UNC-71† | 5 | 2 | 2 | 10 | ||||
(A) Rescue of unc-71(ju156) D neuron axonal defects by tissue specific promoters driving the unc-71 minigene. unc-71(ju156) animals display 48±8% axon guidance defects,scored as `no rescue'.
(B) Functional analysis of UNC-71. UNC-71(M-), metalloprotease domain deletion construct; UNC-71(ECD), extracellular domains of UNC-71 only. Twenty to 50 animals were scored for each transgenic line, and the average percentage was presented.
Degree of rescue is represented by % axon dorsal guidance defects. The dorsal guidance defects of the D neuron axons, visualized by juIs76,were quantified by counting the number of commissures from DD2-6and VD1,3-13 that did not reach the dorsal cord within a single animal, which was then divided by 17 to convert to the percentage of defects
DNAs were injected at 20 ng/μl and 100 ng/μl, and the results were similar. Only the results from the 20 ng/μl DNA injection are presented