Molecular lesions of unc-71 mutants
| Allele . | Nucleotide change . | Amino acid change . | Domains . |
|---|---|---|---|
| ju161 | GGA→AGA | G149R | Prodomain |
| ju156 | 170 bp deletion | G149 | Prodomain |
| ay64 | GAT→AAT | D461N | Disintegrin |
| ay46, ay51 | CTC→CCC | L471P | Disintegrin |
| ay17, ad487 | TGT→TAT | C477Y | Disintegrin |
| ay49 | CGA→TGA | R490opal | Disintegrin |
| ju160 | GAC→AAC | D504N | Disintegrin |
| ju159 | TGT→TAT | C509Y | Disintegrin |
| ay45, ay50 | CAA→TAA | Q533ochre | Cys rich |
| ay52 | TGG→TGA | W550opal | Cys rich |
| ju194 | GGA→TGA | G551opal | Cys rich |
| e541 | GCG→ACG | A557T | Cys rich |
| ju157 | GGA→GAA | G594E | Cys rich |
| ay7 | CAA→TAA | Q623ochre | Cys rich |
| ay47 | TCG→TTG | S628L | Cys rich |
| ay44 | TGT→AGT | C687S | EGF like |
| ay48 | CCA→CTA | P902L | Cytoplasmic |
| CAA→TAA | Q948opal | Cytoplasmic | |
| ju255 | AGA→AAA | R990K | Cytoplasmic |
| Allele . | Nucleotide change . | Amino acid change . | Domains . |
|---|---|---|---|
| ju161 | GGA→AGA | G149R | Prodomain |
| ju156 | 170 bp deletion | G149 | Prodomain |
| ay64 | GAT→AAT | D461N | Disintegrin |
| ay46, ay51 | CTC→CCC | L471P | Disintegrin |
| ay17, ad487 | TGT→TAT | C477Y | Disintegrin |
| ay49 | CGA→TGA | R490opal | Disintegrin |
| ju160 | GAC→AAC | D504N | Disintegrin |
| ju159 | TGT→TAT | C509Y | Disintegrin |
| ay45, ay50 | CAA→TAA | Q533ochre | Cys rich |
| ay52 | TGG→TGA | W550opal | Cys rich |
| ju194 | GGA→TGA | G551opal | Cys rich |
| e541 | GCG→ACG | A557T | Cys rich |
| ju157 | GGA→GAA | G594E | Cys rich |
| ay7 | CAA→TAA | Q623ochre | Cys rich |
| ay47 | TCG→TTG | S628L | Cys rich |
| ay44 | TGT→AGT | C687S | EGF like |
| ay48 | CCA→CTA | P902L | Cytoplasmic |
| CAA→TAA | Q948opal | Cytoplasmic | |
| ju255 | AGA→AAA | R990K | Cytoplasmic |
ju mutations were isolated in the screen for axon guidance mutants using juIs76 [Punc-25GFP] marker(Huang et al., 2002). ay mutants were isolated in sem-5 background(Chen et al., 1997). The underlined letters correspond to the nucleotide alterations. The standard single letter code for each amino acid is used.