In utero lethality occurs in Wnt11+/-intercrosses
| . | Genotype . | . | . | ||
|---|---|---|---|---|---|
| Age . | +/+ (%) . | +/- (%) . | -/- (%) . | ||
| E9.5 (n=38) | 18 | 63 | 18 | ||
| E12.5 (n=361) | 24 | 59 | 17 | ||
| E18.5/P1 (n=152) | 32 | 55 | 13 | ||
| . | Genotype . | . | . | ||
|---|---|---|---|---|---|
| Age . | +/+ (%) . | +/- (%) . | -/- (%) . | ||
| E9.5 (n=38) | 18 | 63 | 18 | ||
| E12.5 (n=361) | 24 | 59 | 17 | ||
| E18.5/P1 (n=152) | 32 | 55 | 13 | ||
The frequencies of Wnt11 genotypically wild type(Wnt11+/+), heterozygous (Wnt11+/-)and mutant homozygous (Wnt11-/-) embryos at E9.5, E12.5 and combined E18.5/P1 pups are shown. Frequencies were determined by Southern blot and PCR genotyping. Wnt11-/- are first recovered in non-Mendelian ratios beginning at E12.5 (χ2 test, P<0.001). The frequencies of Wnt11-/- continue to decline by P1. Homozygous newborn Wnt11-/- animals all die within 48 hours of birth for unknown reasons.