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Table 3.

Summary of RA bead grafting studies

Whole-mount in situ hybridisation for
Islet2
Hb9
Hoxa3
Hoxd4
StageGraftInductionnInductionnInductionnInductionnT
9/10 RA1 in r2r3 none none nd  nd  
11 RA1 in r3r4 none none none none 18 
9/10 RA1 in r3r4 r4 r4 r4 r4 3/7 17 
10 RA5 in r3r4 nd  r4* nd  r4 1/3 
          50 
Whole-mount in situ hybridisation for
Islet2
Hb9
Hoxa3
Hoxd4
StageGraftInductionnInductionnInductionnInductionnT
9/10 RA1 in r2r3 none none nd  nd  
11 RA1 in r3r4 none none none none 18 
9/10 RA1 in r3r4 r4 r4 r4 r4 3/7 17 
10 RA5 in r3r4 nd  r4* nd  r4 1/3 
          50 

RA1, 1×10-4 M; RA5,5×10-4 M.

*

Some induction was observed also in the contralateral r4

Number of embryos ectopically expressing Hoxd4 in r4/number of embryos analysed

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