Propensity of tagA- cells to differentiate as prestalk cells
| Mixture* . | Ratio . | Total prestalk cells(%)† . | Prestalk cells from marked strain(%)‡ . | % of prestalk cells that are marked§ . |
|---|---|---|---|---|
| 1. Ax4+Ax4 | 1:1 | 15±1.2 | 6.9±0.92 | 46 |
| 9:1 | 15±1.2 | 1.9±0.14 | 12 | |
| 2. Ax4+tagA- | 1:1 | 19±1.2 | 14±0.54 | 72 |
| 9:1 | 15±1.4 | 2.8±0.36 | 18 | |
| 3. tagA-+Ax4 | 1:1 | 20±1.5 | 7.0±0.60 | 35 |
| 9:1 | 27±2.0 | 2.0±0.79 | 7.5 | |
| 4. tagA-+tagA- | 1:1 | 27±2.5 | 14±0.67 | 50 |
| 9:1 | 27±2.5 | 3.1±0.30 | 11 |
| Mixture* . | Ratio . | Total prestalk cells(%)† . | Prestalk cells from marked strain(%)‡ . | % of prestalk cells that are marked§ . |
|---|---|---|---|---|
| 1. Ax4+Ax4 | 1:1 | 15±1.2 | 6.9±0.92 | 46 |
| 9:1 | 15±1.2 | 1.9±0.14 | 12 | |
| 2. Ax4+tagA- | 1:1 | 19±1.2 | 14±0.54 | 72 |
| 9:1 | 15±1.4 | 2.8±0.36 | 18 | |
| 3. tagA-+Ax4 | 1:1 | 20±1.5 | 7.0±0.60 | 35 |
| 9:1 | 27±2.0 | 2.0±0.79 | 7.5 | |
| 4. tagA-+tagA- | 1:1 | 27±2.5 | 14±0.67 | 50 |
| 9:1 | 27±2.5 | 3.1±0.30 | 11 |
Data from two separate mixing experiments in which one experiment shows the total percentage of ecmA-positive prestalk cells (3rdcolumn) and the other (4th column) shows the percentage of prestalk cells from a mixture in which only one of the two strains was marked(underlined in 1st column)
The total prestalk cell population was determined by mixing the two strains where both strains express green fluorescent protein (GFP) under the promoter control of the prestalk specific gene ecmA (ecmA/GFP).
In these experiments, only the strain underlined in column 1 was marked with ecmA/GFP to determine the origin of the prestalk cells in the mixture
An estimate of the percentage of the prestalk cells that originated from the marked (underlined) strain in each mixture was obtained by dividing the percentage in the fourth column by the percentage in the third column. In control experiments 1 and 4, the expected percentage would be 50% and 10% for the 1:1 and 9:1 mixture ratios, respectively