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Table 1.

Dictyostelium strains used in this study

StrainDirect parentDrug markersReference
Ax4* Ax3 Knecht et al., 1986  
Ax4 (ecmA/GFPAx4 neor Good and Kuspa, 2000  
Ax4 (cotB/GFPAx4 neor Good and Kuspa, 2000  
Ax4 (act15/GFPAx4 neor This work 
tagA- Ax4 bsr This work 
tagA- (ecmA/GFPAx4 (ecmA/GFPneor, bsr This work 
tagA- (cotB/GFPAx4 (cotB/GFPneor, bsr This work 
tagA- (act15/GFPAx4 (act15/GFPneor, bsr This work 
Ax2 (ecmO/lacZAx2 neor Early et al., 1993  
tagA- (ecmO/lacZAx4 (ecmO/lacZneor, bsr This work 
Ax4 (tagA/tagAneor This work 
tagA- (tagA/tagAtagA- neor, bsr This work 
Ax4 (tagA/lacZAx4 neor This work 
tagA- (tagA/lacZtagA- neor, bsr This work 
Ax4 (cotB/lacZAx4 neor Fosnaugh and Loomis, 1993  
tagA- (cotB/lacZAx4 (cotB/lacZneor, bsr This work 
Ax4 (ecmB/lacZAx4 neor This work 
tagA- (ecmB/lacZAx4 (ecmB/lacZneor, bsr This work 
StrainDirect parentDrug markersReference
Ax4* Ax3 Knecht et al., 1986  
Ax4 (ecmA/GFPAx4 neor Good and Kuspa, 2000  
Ax4 (cotB/GFPAx4 neor Good and Kuspa, 2000  
Ax4 (act15/GFPAx4 neor This work 
tagA- Ax4 bsr This work 
tagA- (ecmA/GFPAx4 (ecmA/GFPneor, bsr This work 
tagA- (cotB/GFPAx4 (cotB/GFPneor, bsr This work 
tagA- (act15/GFPAx4 (act15/GFPneor, bsr This work 
Ax2 (ecmO/lacZAx2 neor Early et al., 1993  
tagA- (ecmO/lacZAx4 (ecmO/lacZneor, bsr This work 
Ax4 (tagA/tagAneor This work 
tagA- (tagA/tagAtagA- neor, bsr This work 
Ax4 (tagA/lacZAx4 neor This work 
tagA- (tagA/lacZtagA- neor, bsr This work 
Ax4 (cotB/lacZAx4 neor Fosnaugh and Loomis, 1993  
tagA- (cotB/lacZAx4 (cotB/lacZneor, bsr This work 
Ax4 (ecmB/lacZAx4 neor This work 
tagA- (ecmB/lacZAx4 (ecmB/lacZneor, bsr This work 
*

Ax4 is used as the `wild-type' laboratory strain. Ax4 is an axenic derivative of NC4 derived from Ax3 (Knecht et al., 1986)

neor, neomycin resistance; bsr, blasticidin S resistance

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