The Company of Biologists

Table 1.

Dictyostelium strains used in this study

Strain	Direct parent	Drug markers	Reference
Ax4^{^*}	Ax3	-	Knecht et al., 1986
Ax4 (ecmA/GFP)	Ax4	neo^r^{^†}	Good and Kuspa, 2000
Ax4 (cotB/GFP)	Ax4	neo^r	Good and Kuspa, 2000
Ax4 (act15/GFP)	Ax4	neo^r	This work
tagA^-	Ax4	bs^r^{^†}	This work
tagA^- (ecmA/GFP)	Ax4 (ecmA/GFP)	neo^r, bs^r	This work
tagA^- (cotB/GFP)	Ax4 (cotB/GFP)	neo^r, bs^r	This work
tagA^- (act15/GFP)	Ax4 (act15/GFP)	neo^r, bs^r	This work
Ax2 (ecmO/lacZ)	Ax2	neo^r	Early et al., 1993
tagA^- (ecmO/lacZ)	Ax4 (ecmO/lacZ)	neo^r, bs^r	This work
Ax4 (tagA/tagA)	-	neo^r	This work
tagA^- (tagA/tagA)	tagA^-	neo^r, bs^r	This work
Ax4 (tagA/lacZ)	Ax4	neo^r	This work
tagA^- (tagA/lacZ)	tagA^-	neo^r, bs^r	This work
Ax4 (cotB/lacZ)	Ax4	neo^r	Fosnaugh and Loomis, 1993
tagA^- (cotB/lacZ)	Ax4 (cotB/lacZ)	neo^r, bs^r	This work
Ax4 (ecmB/lacZ)	Ax4	neo^r	This work
tagA^- (ecmB/lacZ)	Ax4 (ecmB/lacZ)	neo^r, bs^r	This work

Strain	Direct parent	Drug markers	Reference
Ax4^{^*}	Ax3	-	Knecht et al., 1986
Ax4 (ecmA/GFP)	Ax4	neo^r^{^†}	Good and Kuspa, 2000
Ax4 (cotB/GFP)	Ax4	neo^r	Good and Kuspa, 2000
Ax4 (act15/GFP)	Ax4	neo^r	This work
tagA^-	Ax4	bs^r^{^†}	This work
tagA^- (ecmA/GFP)	Ax4 (ecmA/GFP)	neo^r, bs^r	This work
tagA^- (cotB/GFP)	Ax4 (cotB/GFP)	neo^r, bs^r	This work
tagA^- (act15/GFP)	Ax4 (act15/GFP)	neo^r, bs^r	This work
Ax2 (ecmO/lacZ)	Ax2	neo^r	Early et al., 1993
tagA^- (ecmO/lacZ)	Ax4 (ecmO/lacZ)	neo^r, bs^r	This work
Ax4 (tagA/tagA)	-	neo^r	This work
tagA^- (tagA/tagA)	tagA^-	neo^r, bs^r	This work
Ax4 (tagA/lacZ)	Ax4	neo^r	This work
tagA^- (tagA/lacZ)	tagA^-	neo^r, bs^r	This work
Ax4 (cotB/lacZ)	Ax4	neo^r	Fosnaugh and Loomis, 1993
tagA^- (cotB/lacZ)	Ax4 (cotB/lacZ)	neo^r, bs^r	This work
Ax4 (ecmB/lacZ)	Ax4	neo^r	This work
tagA^- (ecmB/lacZ)	Ax4 (ecmB/lacZ)	neo^r, bs^r	This work

^*

Ax4 is used as the `wild-type' laboratory strain. Ax4 is an axenic derivative of NC4 derived from Ax3 (Knecht et al., 1986)

neo^r, neomycin resistance; bs^r, blasticidin S resistance