Timing of ER cluster disassembly versus duration of Ca2+oscillations (Cerebratulus sp.)
| Specimen . | Time at which ER clusters are fully disassembled (minutes post-fertilization) . | Duration of Ca2+ oscillations (minutes post-fertilization) . |
|---|---|---|
| Female 1 | 20.7±1.9 (n=6) | 22.3±5.3 (n=6) |
| Female 2 | 26.7±3.5 (n=8) | 32.0±8.9 (n=8) |
| Specimen . | Time at which ER clusters are fully disassembled (minutes post-fertilization) . | Duration of Ca2+ oscillations (minutes post-fertilization) . |
|---|---|---|
| Female 1 | 20.7±1.9 (n=6) | 22.3±5.3 (n=6) |
| Female 2 | 26.7±3.5 (n=8) | 32.0±8.9 (n=8) |
Several oocytes from two female specimens of Cerebratulus sp. were injected with either DiI to monitor ER clusters or CGDex/RBDex for Ca2+ dynamics (note ER morphology and Ca2+ signaling were not examined within the same oocyte, as the double injections required to load DiI and the Ca2+-sensitive probes tended to reduce oocyte viability compared with singly injected specimens). Timelapse confocal microscopy was begun, and sperm were subsequently added to the specimen dish within ∼30-60 minutes of injection of the fluorescent probe.
n, number of oocytes.