Cooperation of Ogon/Sizzled and Chordin in dorsalization
| RNA . | Ventralized (%) . | Normal (%) . | C1 (%) . | C2 (%) . | C3 (%) . | C4-5 (%) . | n . |
|---|---|---|---|---|---|---|---|
| sizzled 200 pg | 24 | 0 | 0 | 0 | 0 | 76 | 181 |
| chordin 15 pg | 16 | 60 | 14 | 5 | 4 | 1 | 160 |
| chordin 15 pg+sizzled 200 pg | 1 | 0 | 1 | 0 | 3 | 95 | 161 |
| noggin1 50 pg | 0 | 0 | 0 | 0 | 0 | 100 | 73 |
| RNA . | Ventralized (%) . | Normal (%) . | C1 (%) . | C2 (%) . | C3 (%) . | C4-5 (%) . | n . |
|---|---|---|---|---|---|---|---|
| sizzled 200 pg | 24 | 0 | 0 | 0 | 0 | 76 | 181 |
| chordin 15 pg | 16 | 60 | 14 | 5 | 4 | 1 | 160 |
| chordin 15 pg+sizzled 200 pg | 1 | 0 | 1 | 0 | 3 | 95 | 161 |
| noggin1 50 pg | 0 | 0 | 0 | 0 | 0 | 100 | 73 |
Embryos obtained by crossing heterozygous dinott250 fish received injections of ogo/szl and/or chordin RNA. The injected embryos were classified by morphology into categories C1-C5 (where C1 was weak and C5 strong dorsalization) (Mullins et al.,1996). Despite the genotype, the co-injection of both RNAs induced strong dorsalization compared with the single RNA injection of either,indicating that the co-expression of ogo/szl and chordincauses dorsalization in din/din mutant embryos.