Stage-dependent actions of the dominant-negative Tbx5 and Tbx4
| A Effect of misexpression RCAS-EnTbx5 . | . | . | . | |||
|---|---|---|---|---|---|---|
| Stage of infection . | Total . | Number with wingless phenotype . | Number with wing truncations . | |||
| 7 | 10 | 7 (70%) | 2 (20%) | |||
| 8 | 15 | 12 (80%) | 2 (13%) | |||
| 9 | 12 | 8 (67%) | 3 (25%) | |||
| 10 | 13 | 3 (23%) | 5 (38%) | |||
| 11 | 9 | 0 (0%) | 2 (22%) | |||
| 12 | 11 | 0 (0%) | 1 (9%) | |||
| 13 | 10 | 0 (0%) | 1 (10%) | |||
| Totals | 80 | 30 | 16 | |||
| A Effect of misexpression RCAS-EnTbx5 . | . | . | . | |||
|---|---|---|---|---|---|---|
| Stage of infection . | Total . | Number with wingless phenotype . | Number with wing truncations . | |||
| 7 | 10 | 7 (70%) | 2 (20%) | |||
| 8 | 15 | 12 (80%) | 2 (13%) | |||
| 9 | 12 | 8 (67%) | 3 (25%) | |||
| 10 | 13 | 3 (23%) | 5 (38%) | |||
| 11 | 9 | 0 (0%) | 2 (22%) | |||
| 12 | 11 | 0 (0%) | 1 (9%) | |||
| 13 | 10 | 0 (0%) | 1 (10%) | |||
| Totals | 80 | 30 | 16 | |||
| Stage of infection . | Total . | Number with legless phenotype . | Number with leg truncations . |
|---|---|---|---|
| 8 | 6 | 0 (0%) | 0 (0%) |
| 9 | 11 | 0 (0%) | 0 (0%) |
| 11 | 12 | 0 (0%) | 0 (0%) |
| 13 | 7 | 0 (0%) | 0 (0%) |
| Totals | 36 | 0 | 0 |
| Stage of infection . | Total . | Number with legless phenotype . | Number with leg truncations . |
|---|---|---|---|
| 8 | 6 | 0 (0%) | 0 (0%) |
| 9 | 11 | 0 (0%) | 0 (0%) |
| 11 | 12 | 0 (0%) | 0 (0%) |
| 13 | 7 | 0 (0%) | 0 (0%) |
| Totals | 36 | 0 | 0 |
| B Effect of misexpression of RCAS-EnTbx4 . | . | . | . | |||
|---|---|---|---|---|---|---|
| Stage of infection . | Total . | Number with legless phenotype . | Number with leg truncations . | |||
| 7 | 8 | 6 (75%) | 2 (25%) | |||
| 8 | 18 | 11 (61%) | 5 (28%) | |||
| 9 | 12 | 8 (67%) | 3 (33%) | |||
| 10 | 13 | 2 (15%) | 7 (54%) | |||
| 11 | 12 | 0 (0%) | 6 (50%) | |||
| 12 | 14 | 0 (0%) | 2 (14%) | |||
| 13 | 15 | 0 (0%) | 2 (13%) | |||
| Totals | 92 | 27 | 27 | |||
| B Effect of misexpression of RCAS-EnTbx4 . | . | . | . | |||
|---|---|---|---|---|---|---|
| Stage of infection . | Total . | Number with legless phenotype . | Number with leg truncations . | |||
| 7 | 8 | 6 (75%) | 2 (25%) | |||
| 8 | 18 | 11 (61%) | 5 (28%) | |||
| 9 | 12 | 8 (67%) | 3 (33%) | |||
| 10 | 13 | 2 (15%) | 7 (54%) | |||
| 11 | 12 | 0 (0%) | 6 (50%) | |||
| 12 | 14 | 0 (0%) | 2 (14%) | |||
| 13 | 15 | 0 (0%) | 2 (13%) | |||
| Totals | 92 | 27 | 27 | |||
| Stage of infection . | Total . | Number with wingless phenotype . | Number with wing truncations . |
|---|---|---|---|
| 8 | 5 | 0 (0%) | 0 (0%) |
| 9 | 16 | 0 (0%) | 0 (0%) |
| 11 | 10 | 0 (0%) | 0 (0%) |
| 13 | 5 | 0 (0%) | 0 (0%) |
| Totals | 36 | 0 | 0 |
| Stage of infection . | Total . | Number with wingless phenotype . | Number with wing truncations . |
|---|---|---|---|
| 8 | 5 | 0 (0%) | 0 (0%) |
| 9 | 16 | 0 (0%) | 0 (0%) |
| 11 | 10 | 0 (0%) | 0 (0%) |
| 13 | 5 | 0 (0%) | 0 (0%) |
| Totals | 36 | 0 | 0 |
Wingless and legless phenotypes were obtained only when the viruses of the dominant-negative Tbx5 (EnTbx5) and Tbx4(EnTbx4) were infected between stages 7 and 10. Viral injection at later stages (stage 10 to 13) induced limb truncation at a lower rate,revealing a critical period between stages 7 and 10. By contrast,misexpression of EnTbx5 in the leg and EnTbx4 in the wing did not induce morphological alteration even when infected at early stages.