Timing of developmental and behavioral events at adult ecdysis(eclosion) in KO animals
| Event . | KO† . | Control . |
|---|---|---|
| Tracheal filling start - eclosion end | 73.6±8.1 | 67.2±2.1 (ns) |
| Tracheal filling start - end | 1.4±0.6 | (0)‡(*) § |
| Tracheal filling end -EP¶ | 59.4±7.7 | 60.9±3.7 (ns) |
| Tracheal filling end - eclosion start | 59.8±7.7 | 66.3±1.1 (ns) |
| EP - eclosion start | 0.36±0.1 | 3.1±2.4(*) |
| No of head movements prior to EP | 18.1±9.3 | 33.6±4.3 (ns) |
| Eclosion start - eclosion end | 10.0±2†† | 0.91±0.3(**) |
| No. of ecdysis peristalses until eclosion | 85±14†† | 26±2(**) |
| No. of ecdysis peristalses during 1st minute‡‡ | 9.8±0.9†† | 26±2(**) |
| Event . | KO† . | Control . |
|---|---|---|
| Tracheal filling start - eclosion end | 73.6±8.1 | 67.2±2.1 (ns) |
| Tracheal filling start - end | 1.4±0.6 | (0)‡(*) § |
| Tracheal filling end -EP¶ | 59.4±7.7 | 60.9±3.7 (ns) |
| Tracheal filling end - eclosion start | 59.8±7.7 | 66.3±1.1 (ns) |
| EP - eclosion start | 0.36±0.1 | 3.1±2.4(*) |
| No of head movements prior to EP | 18.1±9.3 | 33.6±4.3 (ns) |
| Eclosion start - eclosion end | 10.0±2†† | 0.91±0.3(**) |
| No. of ecdysis peristalses until eclosion | 85±14†† | 26±2(**) |
| No. of ecdysis peristalses during 1st minute‡‡ | 9.8±0.9†† | 26±2(**) |
Values are averages±s.e.m.; n=10 for each group unless noted. Units are minutes or number, depending on the event.
Air filling occurred in a few seconds in the controls and could not be measured precisely.
(ns) P>0.05;
0.01<P<0.05;
P<0.01. Student's t-test.
EP, extended ptilinum (see Fig. 6A,B).
n=9; one animal failed to eclose.
Approximate time for control to eclose (0.91±0.3 minutes; see“Eclosion start - eclosion end”, above).