Timing of developmental and behavioral events at pupal ecdysis in KO animals
| Event . | KO† . | Control . |
|---|---|---|
| Pre-ecdysis | 24.1±1.3 | 9.35±0.49(**) ‡ |
| No. of pre-ecdysis sweeps | 20.8±1.4 | 9.4±1.0(**) |
| Frequency of pre-ecdysis sweeps | 0.88±0.06 | 1.02±0.13 (ns) |
| Ecdysis | -§ | 0.94±0.21 |
| No. of ecdysis sweeps | - | 5.1±0.5 |
| Post ecdysis period¶ | 75.8±5.9 | 125.9±7.5(**) |
| No. of postecdysis contractions, first 30 minutes | 17.3±0.7 | 20.2±1.0(*) |
| Average length of prothoracic pair of legs†† | 1.09±0.01 | 1.53±0.01(**) |
| Average length of wings‡‡ | 0.95±0.01 | 1.12±0.01(**) |
| Event . | KO† . | Control . |
|---|---|---|
| Pre-ecdysis | 24.1±1.3 | 9.35±0.49(**) ‡ |
| No. of pre-ecdysis sweeps | 20.8±1.4 | 9.4±1.0(**) |
| Frequency of pre-ecdysis sweeps | 0.88±0.06 | 1.02±0.13 (ns) |
| Ecdysis | -§ | 0.94±0.21 |
| No. of ecdysis sweeps | - | 5.1±0.5 |
| Post ecdysis period¶ | 75.8±5.9 | 125.9±7.5(**) |
| No. of postecdysis contractions, first 30 minutes | 17.3±0.7 | 20.2±1.0(*) |
| Average length of prothoracic pair of legs†† | 1.09±0.01 | 1.53±0.01(**) |
| Average length of wings‡‡ | 0.95±0.01 | 1.12±0.01(**) |
Values are averages±s.e.m.; n=10 for each group. Units are minutes or number, or mm, depending on the measurement.
(ns) P>0.05;
0.01<P<0.05;
P<0.01. Student's t-test.
Behaviors resembling ecdysis were not observed.
Period between the end of head eversion [ecdysis (for controls) or pre-ecdysis sweeps (for KO larvae)] and the end of body contractures.
Measured on pharate adults, from joint at the level of the neck; one prothoracic leg was measured per animal; n=23 per group.
Measured on pharate adults, one wing easured per group. n=20 per group.