Injection of RALDH2 mRNA rescues tbx5.1 expression innls
. | . | Number of observed phenotypes . | . | . | ||||
|---|---|---|---|---|---|---|---|---|
| Experiment . | Total number of embryos . | Wild type (% of total) . | Weak expression (% of total) . | No expression (% of total) . | ||||
| A | 40 | 29 | 6 | 5 | ||||
| B | 21 | 13 | 7 | 0 | ||||
| C | 45 | 38 | 7 | 0 | ||||
| D | 40 | 32 | 7 | 1 | ||||
| Sum | 146 (100%) | 112 (77%) | 27 (18%) | 6 (4%) | ||||
| Theoretically expected | 146 (100%) | 110 (75%) | 0 | 36 (25%) | ||||
| Microinjection of RALDH2 mRNA into the progeny of nls/WIK heterozygous parents. Selected embryos with missing or partial expression oftbx5.1 in the pectoral fin buds was assayed at 24 hpf. Putatively rescued animals were genotyped and confirmed as being nls homozygotes using SSLP markers. Expression in pectoral fins was assayed as `weak' when less strong than retinal expression in the same specimen. Percentages rounded to the nearest 1%. Uninjected batches were kept as controls. | ||||||||
. | . | Number of observed phenotypes . | . | . | ||||
|---|---|---|---|---|---|---|---|---|
| Experiment . | Total number of embryos . | Wild type (% of total) . | Weak expression (% of total) . | No expression (% of total) . | ||||
| A | 40 | 29 | 6 | 5 | ||||
| B | 21 | 13 | 7 | 0 | ||||
| C | 45 | 38 | 7 | 0 | ||||
| D | 40 | 32 | 7 | 1 | ||||
| Sum | 146 (100%) | 112 (77%) | 27 (18%) | 6 (4%) | ||||
| Theoretically expected | 146 (100%) | 110 (75%) | 0 | 36 (25%) | ||||
| Microinjection of RALDH2 mRNA into the progeny of nls/WIK heterozygous parents. Selected embryos with missing or partial expression oftbx5.1 in the pectoral fin buds was assayed at 24 hpf. Putatively rescued animals were genotyped and confirmed as being nls homozygotes using SSLP markers. Expression in pectoral fins was assayed as `weak' when less strong than retinal expression in the same specimen. Percentages rounded to the nearest 1%. Uninjected batches were kept as controls. | ||||||||