L3 (at 3, 7 and 11 days post-infection -dpi-) and L4 (at 14 dpi) were stained with propidium iodide and phalloidin, the Wolbachia localization along the A-P axis was established by confocal microscopy, and their density, established as propidium iodide foci, was scored into arbitrary categories ranging from 0 to 4 as follows: 0  =  no Wolbachia; 1  =  few, scattered Wolbachia; 2  =  numerous Wolbachia; 3  =  very numerous (i.e. ∼100(s) around each nucleus), 4  =  the cytoplasm is filled with Wolbachia.