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Keywords: voltage-clamp
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Journal Articles
J Exp Biol (2001) 204 (21): 3717–3726.
Published: 1 November 2001
... (myofibres). Values are means ± s.e.m . (D) Voltage-clamp recording of total membrane currents from a cell dissociated at 20–22 °C showing that both inward and outward current amplitudes are low. The stimulus protocol was 25 ms test pulses, incrementing in 10 mV steps from −70 to +90 mV from a holding...
Journal Articles
J Exp Biol (1995) 198 (12): 2537–2545.
Published: 1 December 1995
.... For recording of ciliary beating, a high-frequency video system (NAC, Tokyo) was used. Image frequency was 200 Hz and a single flash of stroboscobic illumination lasted 15 μ s. During the experiments, a specimen was voltage-clamped at a holding potential equal to the resting potential (approximately -35 mV...
Journal Articles
J Exp Biol (1995) 198 (7): 1483–1492.
Published: 1 July 1995
... activity, suggesting an important role for the inward Ca 2+ current in maintaining a regular swimmeret rhythmic activity in crayfish. 17 03 1995 © The Company of Biologists Limited 1995 crayfish swimmeret system motor neurones voltage-clamp Pacifastacus leniusculus...
Journal Articles
J Exp Biol (1994) 186 (1): 157–171.
Published: 1 January 1994
.... This outward current is not voltage-dependent in the range -80 mV to -10 mV, as shown in voltage-clamp experiments by a linear current–voltage relationship. The reversal potential of this current is approximately -60 mV, indicating that chloride or potassium ions underlie the current. Using dynamic-clamp, we...
Journal Articles
J Exp Biol (1993) 185 (1): 267–286.
Published: 1 December 1993
...Kevin L. Blair; Peter A. V. Anderson ABSTRACT Cells were dispersed from the brains of the triclad flatworm Bdelloura candida and maintained in primary culture for up to 2 weeks. Cultured cells assumed a variety of morphologies consistent with those of neurones in vivo . Whole-cell voltage-clamp...
Journal Articles
J Exp Biol (1992) 173 (1): 75–90.
Published: 1 December 1992
...R. J. Martin; P. Thorn; K. A. F. Gration; I. D. Harrow ABSTRACT Voltage-activated currents in cell bodies of the somatic muscle cells of Ascaris suum were studied using a two-microelectrode voltage-clamp technique. Cells recorded from had resting membrane potentials around −35 mV and had input...
Journal Articles
Journal Articles
J Exp Biol (1991) 161 (1): 333–346.
Published: 1 November 1991
... Anthozoa voltage-clamp calcium currents potassium currents myoepithelial cells Calliactis tricolor Cnidarians are the simplest extant organisms to possess a nervous system and as such, have been the focus of much research directed at understanding the properties of the ‘first’ nervous...
Journal Articles
J Exp Biol (1991) 161 (1): 239–255.
Published: 1 November 1991
... by Company of Biologists 1991 membrane potential voltage-clamp cirral movement Stylonychia mytilus The ciliary motor response in ciliates is closely coupled to membrane potential changes ( Machemer, 1986 , 1990 ). In Paramecium and Stylonychia , it has been established that membrane...
Journal Articles
J Exp Biol (1991) 155 (1): 203–217.
Published: 1 January 1991
...Julian P. L. Davis; Robert M. Pitman ABSTRACT The effects of a number of dopaminergic agonists and antagonists upon the soma of a prothoracic inhibitory motoneurone of the cockroach (Periplaneta americana) have been recorded under voltage-clamp conditions. Dopamine generates inward currents...
Journal Articles
Journal Articles
J Exp Biol (1990) 151 (1): 387–403.
Published: 1 July 1990
... suggest that the Na + channels of DUM neurones have more than one open state and/or more than one inactivated state. * To whom reprint requests should be sent. 27 02 1990 © 1990 by Company of Biologists 1990 patch-clamp voltage-clamp Na + current cockroach adult isolated...
Journal Articles
J Exp Biol (1989) 141 (1): 1–20.
Published: 1 January 1989
...R. R. Stewart; J. G. Nicholls; W. B. Adams ABSTRACT Na + , K + and Ca 2+ currents have been measured by voltage-clamp in Retzius (R), anterior pagoda (AP) and sensory (pressure, touch and nociceptive) cells dissected from the central nervous system (CNS) of the leech. These cells maintain...
Journal Articles
Journal Articles
J Exp Biol (1987) 133 (1): 215–230.
Published: 1 November 1987
...Peter A. V. Anderson; M. Craig Mckay ABSTRACT Electrical properties of cnidocytes isolated from the hydroid Cladonema and the scyphomedusa Chrysaora were examined using current- and voltage-clamp recording techniques. The stenoteles of Cladonema produced action potentials when depolarized above 0mV...
Journal Articles
J Exp Biol (1987) 133 (1): 231–248.
Published: 1 November 1987
...Peter A. V. Anderson ABSTRACT Neurones of the motor nerve net of the jellyfish Cyanea capillata were axotomized, and voltage-clamped using the whole-cell configuration of the patchclamp technique. Outward currents were blocked by a combination of extracellular 4-aminopyridine (4-AP...
Journal Articles
J Exp Biol (1985) 117 (1): 369–388.
Published: 1 July 1985
... and delayed outward membrane currents in isolated neural somata under voltage clamp . J. Physiol.,Lond . 213 , 1 – 19 . 10.1113/jphysiol.1971.sp009364 Connor , J. A. & Stevens , C. F. ( 1971b ). Voltage clamp studies of transient outward membrane current in gastropod neural somata...
Journal Articles