... reticulum; (2) mutants in which the conformational equilibrium has been shifted from the E 2 state, characterized by high affinity for vanadate, to the E 1 state, characterized by high affinity for ATP; and (3) mutants with altered coupling between ATP hydrolysis and proton pumping. Although much remains...

... mechanism. The sensitivity of ATP-dependent Ca 2+ transport to vanadate indicated that it was probably due to a P-type ATPase. This system exhibited an extremely high apparent affinity for Ca 2+ ( K t =65.28±14.39 nmol l −1 ; J max =1.07±0.06 pmol µg −1 pro-tein 8 s −1 ). The Na + -gradient-dependent...

... + ], and Sch28080 (10 −4 mol l −1 ), a K + /H + -ATPase inhibitor, were without effect on fluid secretion. Dihydro-ouabain was also without effect on electrical potential differences either in the absence or in the presence of Ba 2+ . Vanadate (10 −3 mol l −1 ), in contrast, strongly reduced fluid secretion...

...Hong Lin; David Randall ABSTRACT Ion transport inhibitors, amiloride, SITS, vanadate and acetazolamide, were added to the water to determine the effect of ion transfer mechanisms on the acidification of water passing over the gills. In neutral water, proton excretion causes a marked reduction...