... prevent or clear accumulations of brood at the branchial basket, which would otherwise hinder breathing by the parent. Dual-view videos of female Nile tilapias ( Oreochromis niloticus ) during mouthbrooding showed that churning involves a posterior-to-anterior wave of expansion and compression of the head...

...Benjamin P. Moorman; Darren T. Lerner; E. Gordon Grau; Andre P. Seale ABSTRACT This study characterizes the differences in osmoregulatory capacity among Mozambique tilapia, Oreochromis mossambicus , reared in freshwater (FW), in seawater (SW) or under tidally driven changes in salinity...

...Alison M. Gardell; Jun Yang; Romina Sacchi; Nann A. Fangue; Bruce D. Hammock; Dietmar Kültz SUMMARY This study aimed to determine the regulation of the de novo myo -inositol biosynthetic (MIB) pathway in Mozambique tilapia ( Oreochromis mossambicus ) brain following acute (25 ppt) and chronic (30...

... produced by heterospecifics, we investigated possible differences in the odour of bile between the sexes and among different species – the eel ( Anguilla anguilla ), goldfish ( Carassius auratus ) and Mozambique tilapia ( Oreochromis mossambicus ) – using the electro-olfactogram (EOG). We also identified...

...Diego F. Fiol; Enio Sanmarti; Romina Sacchi; Dietmar Kültz SUMMARY A novel tilapia prolactin (PRL) receptor ( Om PRLR2) was identified based on its induction during hyperosmotic stress. Om PRLR2 protein shows 28% identity to tilapia Om PRLR1 and 26% identity to human PRLR. Comparison of Om PRLR1...

... and are involved in active ion absorption or secretion. The objectives of this study were to clone and identify `freshwater-type' and `seawater-type' cation–chloride cotransporters of euryhaline Mozambique tilapia ( Oreochromis mossambicus ) and to determine their intracellular localization patterns within...

...Jen-Chieh Shiao; Su-Mei Wu; Yi-Ping Hwang; Done-Ping Wu; Pung-Pung Hwang SUMMARY Thyroid-mediated otolith growth in tilapia was evaluated by the ontogenic triiodothyronine (T 3 ) profile revealed by radioimmunoassay during the first month after hatching. Thyroid hormone receptor genes( TR α and TR...

.... Such heterotopic thyroid follicles are active,and considered to work in concert with the subpharyngeal thyroid. In Mozambique tilapia ( Oreochromis mossambicus ) thyroid activity is,indeed, restricted to the subpharyngeal region; in common carp ( Cyprinus carpio ) the functional endocrine thyroid is associated...

...Jennifer C. Smith; S. Laurie Sanderson SUMMARY Filtration mechanisms are known for only two species of suspension-feeding tilapia, each of which relies on a different method of particle retention. We used high-speed video endoscopy to assess whether a third species of tilapia, Oreochromis aureus...

...Diego F. Fiol; Stephanie Y. Chan; Dietmar Kültz SUMMARY Mechanisms of induction of osmotic stress transcription factor 1 (Ostf1)were analyzed in gill epithelium of tilapia exposed to salinity stress. Experiments with primary cultures of gill epithelial cells revealed that hyperosmotic Ostf1...

... of Mozambique tilapia embryos and subjected to in vitro incubation. To evaluate the ion-transporting property of the yolk balls, we examined Cl – content and turnover in yolk balls incubated in freshwater and seawater for 48 h, and distribution patterns of three ion transporters,Na + /K + -ATPase,Na + /K + /2Cl...

...Soichi Watanabe; Toyoji Kaneko; Katsumi Aida SUMMARY We have cloned a homologue of mammalian aquaporin-3 (AQP3) from gills of Mozambique tilapia using a reverse transcription-polymerase chain reaction(RT-PCR). The deduced amino acid sequence shared 64–75% homology with other vertebrate AQP3...

...Junya Hiroi; Stephen D. McCormick; Ritsuko Ohtani-Kaneko; Toyoji Kaneko SUMMARY Mozambique tilapia Oreochromis mossambicus embryos were transferred from freshwater to seawater and vice versa , and short-term changes in the localization of three major ion transport proteins,Na + /K + -ATPase,Na + /K...

...Li-Yih Lin; Pung-Pung Hwang SUMMARY Mitochondria-rich cells (MRCs) in the yolk-sac membrane of tilapia( Oreochromis mossambicus ) larvae were examined by Na + /K + -ATPase immunocytochemistry and vital staining for glycoproteins following acclimation to high (7.5–7.9 mmol l –1 ), normal (0.48–0.52...

...Masaya Morita; Akihiro Takemura; Makoto Okuno SUMMARY Euryhaline tilapia Oreochromis mossambicus can reproduce in freshwater and in seawater. Regulation of sperm motility appears to be modulated during acclimation of the fish from freshwater to seawater, being independent of extracellular Ca 2...

...Kiyono Shiraishi; Junya Hiroi; Toyoji Kaneko; Manabu Matsuda; Tetsuya Hirano; Takao Mori SUMMARY To examine the functional differentiation of chloride cells in the yolk-sac membrane of tilapia ( Oreochromis mossambicus ) embryos, we developed a ‘yolk-ball’ incubation system in which the yolk sac...

...Junya Hiroi; Toyoji Kaneko; Masaru Tanaka ABSTRACT Changes in chloride cell morphology were examined in the yolk-sac membrane of Mozambique tilapia ( Oreochromis mossambicus ) embryos and larvae transferred from fresh water to sea water. By labelling chloride cells with DASPEI, a fluorescent probe...

...J. C. Fenwick; S. E. Wendelaar Bonga; G. Flik ABSTRACT In vivo treatment with external bafilomycin A 1 , a selective inhibitor of V-ATPase H + pumps, reduced whole-body Na + influx by up to 90 % in young tilapia and 70 % in young carp. The inhibition was rapidly reversible, with whole-body Na...

...Jong-Chang Tsai; Pung-Pung Hwang ABSTRACT The organization of the microtubules and actin filaments in the gills of tilapia ( Oreochromis mossambicus ) larvae was revealed by confocal microscopy. The fluorescence intensity of the microtubules in the gills was increased by adding wheat germ...

..., partially characterised and tested for their inhibitory effect on the K + -dependent p -nitrophenol phosphatase ( p NPPase) activity of tilapia ( Oreochromis mossambicus ) gill basolateral membrane. Thin-layer chromatography of the lipids from dichloromethane:methanol extracts of M. aeruginosa PCC 7820...