...Bettina Schewe; Wolfgang Blenau; Bernd Walz SUMMARY Salivary gland cells of the blowfly Calliphora vicina have a vacuolar-type H + -ATPase (V-ATPase) that lies in their apical membrane and energizes the secretion of a KCl-rich primary saliva upon stimulation with serotonin (5-hydroxytryptamine...

...Cathleen Rotte; Jeannine Witte; Wolfgang Blenau; Otto Baumann; Bernd Walz SUMMARY Cockroach salivary glands are innervated by dopaminergic and serotonergic neurons. Both transmitters elicit saliva secretion. We studied the distribution pattern of neurons containing γ-aminobutyric acid (GABA...

...Eric Calvo; José M. C. Ribeiro SUMMARY Previous analysis of the salivary gland transcriptome of Culex quinquefasciatus showed the potential presence of an endonuclease with sequence similarities to shrimp, crab and two tsetse salivary proteins. Indeed, not only was the cloned cDNA shown to encode...

... of their saliva to find blood. Here we describe the first evidence of vasodilators in horse fly Hybomitra bimaculata (Diptera, Tabanidae) salivary gland extract and clone and express one of the active peptides (termed vasotab). Physiological tests using crude salivary gland extracts and reverse-phase HPLC...

...Mayumi Akaki; James A. Dvorak SUMMARY Sporozoite invasion of mosquito salivary glands is critical for malaria transmission to vertebrate hosts. After release into the mosquito hemocoel,the means by which malaria sporozoites locate the salivary glands is unknown. We developed a Matrigel-based...

...Otto Baumann; Dana Kühnel; Petra Dames; Bernd Walz SUMMARY The paired salivary glands in the cockroach are composed of acini with ion-transporting peripheral P-cells and protein-secreting central C-cells, and a duct system for the modification of the primary saliva. Secretory activity...

... in transgenic mosquitoes will make the selection of resistant parasites unlikely. References Arcá, B., Lombardo, F., de Lara Capurro Guimarães,M., della Torre, A., Dimopoulos, G., James, A. A. and Coluzzi, M. ( 1999 ). Trapping cDNAs encoding secreted proteins from the salivary glands of the malaria...

...Jesus G. Valenzuela; Ivo M. B. Francischetti; Van My Pham; Mark K. Garfield; Thomas N. Mather; José M. C. Ribeiro SUMMARY To attempt description of the set of mRNA and protein (sialome) expressed in the salivary glands of the tick Ixodes scapularis , we randomly sequenced 735 clones of a full...

...Ivo M. B. Francischetti; Jesus G. Valenzuela; Van My Pham; Mark K. Garfield; José M. C. Ribeiro SUMMARY Hundreds of Anopheles gambiae salivary gland cDNA library clones have been sequenced. A cluster analysis based on sequence similarity at e -60 grouped the 691 sequences into 251 different...

... clones derived from the salivary gland mRNA from female mosquitoes. The full sequence of the cDNA demonstrated homology between A. albimanus salivary peroxidase and several members of the myeloperoxidase gene family. A close comparison of A. albimanus salivary peroxidase with canine myeloperoxidase...

... intracellular recordings from isolated salivary glands. Dopamine evokes a slow, almost tonic, and reversible dose-dependent elevation in [Ca 2+ ] i in the duct cells. Upon stimulation with 10 −6 mol l −1 dopamine, [Ca 2+ ] i rises from 48±4 nmol l −1 to 311±43 nmol l −1 (mean ± S.E.M., N =18) within 200–300 s...

...Mary S. Cupp; José M. C. Ribeiro; Donald E. Champagne; Eddie W. Cupp ABSTRACT A cDNA was cloned from the salivary glands of a blood-feeding black fly Simulium vittatum . The encoded protein has been given the name Simulium vittatum erythema protein or SVEP, because of its ability to increase blood...

...Declan W. Ali ABSTRACT Insect salivary glands are glands associated with nutrient intake whose secretions are generally involved in the digestion and lubrication of food. They are under the control of neuroactive substances and may be innervated from several sources including the suboesophageal...

...Mark G. Novak; José M. C. Ribeiro; John G. Hildebrand ABSTRACT A dense plexus of axons, immunoreactive to antisera against 5-hydroxytryptamine (5-HT, serotonin) and surrounding the proximal medial lobe of the salivary gland of adult female Aedes aegypti mosquitoes, was demonstrated by means...

...Werner A. Wuttke; Thomas Munsch; Michael S. Berry ABSTRACT Intracellular pH (pHi) and membrane potential (E m ) of giant salivary gland cells of the leech, Haementeria ghilianii, were measured with double-barrelled, neutral-carrier, pH-sensitive microelectrodes. E m was -51±11.2 mV and pHi was 6.98...

...A. N. Gifford; R. A. Nicholson; R. M. Pitman ABSTRACT The salivary glands of the cockroach and locust are innervated primarily from two pairs of motoneurones, designated SN1 and SN2, in the suboesophageal ganglion. Intracellular cobalt fills and subsequent silver intensification were used to reveal...

... that 5-HT may exert its actions via cyclic AMP. 12. The possible role of 5-HT in salivary secretion is discussed. 03 01 1991 © 1991 by Company of Biologists 1991 salivary gland leech Haementeria ghilianii 5-hydroxytryptamine acetylcholine cyclic nucleotides action potential...

...Werner A. Wuttke; Roy T. Sawyer; Michael S. Berry ABSTRACT Each salivary gland cell of Haementeria extends a single process, or ductule, anteriorly into the proboscis; secretory products are released at the ductule ending. Some ductules secrete into the lumen of the proboscis and others...

... in the presence of serotonin, and secretion is completely abolished by cobalt. Perfusion with serotonin excites the salivary gland cells into impulse activity, and often evokes bursting. Impulse activity of the peripherally projecting, serotonergic Retzius cells evokes both depolarizations and action potentials...

...Andrew Barber ABSTRACT Evidence is presented to show that the electrical activity of the salivary glands of Philine aperta is controlled by two largely independent systems which elicit different responses from salivary acinar cells. The excitatory junction potentials (EJPs) recorded from salivary...