... of pavement cells (PVCs) were used to examine TJ protein transcript abundance during the development of epithelium confluence and epithelium resistive properties. Differences in TJ protein expression patterns and transcript abundance between gill models composed of PVCs and models composed of PVCs...

...Adolf Michael Sandbichler; Margit Egg; Thorsten Schwerte; Bernd Pelster SUMMARY Permeability of rainbow trout gill pavement cells cultured on permeable supports (single seeded inserts) changes upon exposure to freshwater or treatment with cortisol. The molecular components of this change...

... of the present study, we aimed to clone the Ostf cDNA from gills of Japanese eels. The expression profiles of Ostf mRNA upon freshwater-to-seawater transfer, and vice versa , were measured in Percoll™-gradient-isolated pavement cells (PVCs) and chloride cells(CCs). Additionally, we investigated the regulation...

... cultures and whole gill sections strongly suggest that ECaC is not exclusive to mitochondria-rich cells but that it is also found in pavement cells. Not only was ECaC protein localized to areas of the gill normally having few mitochondria-rich cells, but there was also no consistent co-localization of ECaC...

...) cell, intercalated MR cell, chloride cell and pavement cell] differ in several respects from those of teleosts. The ultrastructural characteristics of these cells are distinctive and closely resemble those of certain ion-transporting epithelia in other vertebrates, for which the function has been...

...Jie Li; Jelle Eygensteyn; Robert A. C. Lock; Sjoerd E. Wendelaar Bonga; Gert Flik ABSTRACT Chloride cells (CCs; recognised by the vital mitochondrial stain DASPEI) and pavement cells (PCs) isolated from tilapia opercular epithelium were adhered to Cell-Tak-coated glass coverslips and loaded...

... and cytoplasm. Electron microscopy revealed that specific immunoreactivity was localized to the pavement cells and was particularly associated with the apical membrane and subapical cytoplasmic vesicles. The increased H + -ATPase immunoreactivity in the epithelial cells of hypercapnic fish and the increased...

... Fish gill oxidative metabolism cortisol chloride cell pavement cell The fish gill is a physiologically diverse organ that is involved in gas transfer, ion regulation, acid-base balance and nitrogenous waste elimination. The energy requirements of the latter three processes (especially ion...