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J Exp Biol (2021) 224 (3): jeb234708.
Published: 3 February 2021
... flexibility in birds, relying on activity and mRNA data from the same animals. Our model was nestling house sparrows ( Passer domesticus ), already known to modulate intestinal peptidase activity when switching between lower and higher protein diets. Twenty-four hours after a switch from an adequate, lower...
Includes: Supplementary data
Kyle S. MacLea, Ali M. Abuhagr, Natalie L. Pitts, Joseph A. Covi, Brandon D. Bader, Ernest S. Chang, Donald L. Mykles
J Exp Biol (2012) 215 (4): 590–604.
Published: 15 February 2012
.... Gl-Rheb mRNA levels increased 3.4-fold and 3.9-fold during premolt in claw muscles from animals induced to molt by eyestalk ablation (ESA) and multiple leg autotomy (MLA), respectively, and mRNA levels were positively correlated with hemolymph ecdysteroids. There was little or no effect of molting...
J Exp Biol (2010) 213 (11): 1901–1906.
Published: 1 June 2010
...P. J. Ponganis; T. J. Welch; L. S. Welch; T. K. Stockard SUMMARY Increased oxygen storage is essential to the diving capacities of marine mammals and seabirds. However, the molecular mechanisms underlying this adaptation are unknown. Myoglobin (Mb) and Mb mRNA concentrations were analyzed...
J Exp Biol (2009) 212 (1): 78–88.
Published: 1 January 2009
...) transfer. The α 1a and α 1b isoforms were both in the range 1–6 amol 20 ng –1 total RNA; α 1a decreased andα 1b increased after SW-transfer, their ratio changing from 5:1 in FW to 0.26:1 in SW. The α 1c and α 3 levels were 10- and 100-fold lower, respectively. Theβ 1 -subunit mRNA level was 0.1–0.3 amol 20...
J Exp Biol (2007) 210 (18): 3245–3254.
Published: 15 September 2007
... mRNA levels in ES ganglia, gill,hepatopancreas, ovary and testis, and at lower levels in YO, heart and skeletal muscle. Three Gl-GC-II isoforms, which vary in the length of insertions (+18, +9 and +0 amino acids) within the N-terminal ligand-binding domain, differed in tissue distribution. Gl-GC-II(+18...
J Exp Biol (2007) 210 (16): 2905–2911.
Published: 15 August 2007
...-GS04 ) are expressed in the brain of the ammonia-intolerant rainbow trout Oncorhynchus mykiss and that cerebral GSase is induced during ammonia stress. We measured GSase activity and the mRNA expression of Onmy-GS01-GS04 in fore-, mid- and hindbrain and liver, as well as ammonia concentrations...
J Exp Biol (2007) 210 (9): 1497–1506.
Published: 1 May 2007
... al., 2005 ; Carninci, 2006 ). The mRNAs (or non-cloned cDNAs) isolated from tissues are labeled and hybridized to these arrays and the expressed regions of the genome identified from the distribution of positive array probes. These expressed regions are bioinformatically grouped into contiguous...
Includes: Supplementary data
J Exp Biol (2005) 208 (16): 3177–3197.
Published: 15 August 2005
... Caenorhabditis elegans ; domain IV was replaced by a unique `T domain' sequence. It was expressed in most tissues,except eyestalk ganglia and Y-organ. The effects of elevated ecdysteroid,induced by eyestalk ablation, on calpain and ecdysone receptor (Gl-EcR) mRNA levels in skeletal muscles were quantified...
J Exp Biol (2004) 207 (16): 2845–2857.
Published: 15 July 2004
... chain reaction(RT-PCR) and 5′ and 3′ rapid amplification of cDNA ends (RACE) PCR generated a full-length cDNA sequence (3982 bp) of land crab NO synthase(Gl-NOS) from molting gland (Y-organ) and thoracic ganglion mRNA. The open reading frame encoded a protein of 1199 amino acids with an estimated mass...
J Exp Biol (2003) 206 (9): 1511–1521.
Published: 1 May 2003
... of four expressed GSase mRNAs for the tetraploid rainbow trout. For two of these mRNAs( Onmy-GS01, -GS02 ) we characterize the full-length coding regions,and for two others ( Onmy-GS03 , - GS04 ), we describe partial sequences. Northern analysis of Onmy-GS01 , - GS02, -GS03 and -GS04 indicates that (1...
J Exp Biol (2003) 206 (3): 561–575.
Published: 1 February 2003
.... Purification of Ha-CalpM showed that the 62 kDa and 68 kDa isoforms co-eluted from gel filtration and ion exchange columns at positions consistent with those of previously described Ca 2+ -dependent proteinase III(CDP III; 59 kDa). Ha-CalpM mRNA and protein did not change during the moulting cycle. The muscle...
J Exp Biol (2002) 205 (15): 2143–2152.
Published: 1 August 2002
...., 1999 ), an approach that allows large intraspecific size differences to be included. The scarce data on fish show a complex pattern in which not only size but also age and growth rate seem to influence DNA and mRNA concentrations( Burness et al., 1999 ), with translational regulation being implicated...
J Exp Biol (2001) 204 (14): 2517–2525.
Published: 15 July 2001
...% and 71–83% amino acid identity, respectively). In halibut, these opsins are expressed in the pineal organ of embryos and appear in the retina of larvae. Our recent in situ hybridisation studies with RNA probes for HPO1 and HPO4 demonstrate the presence of green-like opsin mRNAs in the pineal organ...