... by transcriptional knockdown of the gene encoding the MR ( mr -KD) using double-stranded RNA. Following mr -KD, a significant reduction in MR protein abundance was observed in the epithelium. The mr -KD epithelium demonstrated reduced transepithelial resistance (TER) and an increase in the paracellular flux of [ 3 H...

...Alex M. Zimmer; Yihang K. Pan; Theanuga Chandrapalan; Raymond W. M. Kwong; Steve F. Perry ABSTRACT Loss-of-function technologies, such as morpholino- and RNAi-mediated gene knockdown, and TALEN- and CRISPR/Cas9-mediated gene knockout, are widely used to investigate gene function and its...

.... rosenbergii . To achieve this, RNAi mediated knockdown as well as augmentation of iag transcripts were effected in ∼60 g OC males using plasmid-based constructs pcD-IAG-lh and pcD-IAGorf, respectively. The treatments were administered to animals maintained in isolation as well as in community. The knockdown...

... shortly after birth; however, a cardiac cause is possible. The zebrafish is an ideal model to investigate the role of fxr1 during development of the heart. We have carried out morpholino knockdown of fxr1 and have demonstrated abnormalities of striated muscle development and abnormal development...

...Donna G. Folk; Luke A. Hoekstra; George W. Gilchrist SUMMARY To explore the correlation of traits linked to thermotolerance, we compared three thermal endpoints (knockdown temperature and two critical thermal maxima) among replicate populations of Drosophila melanogaster selected for high, or low...

...Donna G. Folk; Patty Zwollo; David M. Rand; George W. Gilchrist SUMMARY We studied adaptive thermotolerance in replicate populations of Drosophila melanogaster artificially selected for high and low knockdown temperature ( T KD ), the upper temperature at which flies can no longer remain upright...