... about bimodal breathers, which utilize both water and air. Here, we characterized the influence of temperature on arterial acid–base balance and intracellular pH (pH i ) in the bimodal-breathing swamp eel, Monopterus albus . This teleost uses the buccopharyngeal cavity for gas exchange and has very...

...). Whether and to what extent V-ATPase contributes to intracellular pH (pH i ) regulation in unstimulated gland cells is unknown. We used the fluorescent dye BCECF to study intracellular pH i regulation microfluorometrically and show that: (1) under resting conditions, the application of Na + -free...

... with submicromolar serotonin. In the present study we investigated the mechanisms of strong luminal alkalinization using the intracellular fluorescent indicator BCECF-AM. Following stimulation with serotonin, we observed that intracellular pH (pH i ) of the anterior midgut increased from a mean of 6.89 to a mean...

... cardiac performance was restored in six out of seven hearts upon return to control conditions. Myocardial intracellular pH (pH i ) was protected in situ , as has been found in vivo , and this protection extended to the highest level of CO 2 (7.5%)investigated. Thus, maintained heart function during...

... microfluometrically whether V-ATPase and carbonic anhydrase (CA) are involved in intracellular pH (pH i ) regulation,and we have localized CA activity by histochemistry. We show: (1) mean pH i in salivary gland cells is 7.5±0.3 pH units( N =96), higher than that expected from passive H + distribution; (2) low 5-HT...

... of Physical Chemistry, 14476 Potsdam-Golm, Germany(e-mail: hille@uni-potsdam.de ) 10 12 2007 © The Company of Biologists Limited 2008 2008 dopamine serotonin insect salivary glands ion transport intracellular pH sodium–potassium–chloride cotransporter sodium–proton exchanger anion...

... by a significant increase in inorganic phosphate levels from 0.3±0.3 to 9.5±3.4 mol g –1 , and a drop in intracellular pH from 7.48±0.03 to 6.81±0.05 in muscle. This coincides with the point when the fish change gait from subcarangiform swimming to kick-and-glide bursts. As the number of kicks increased, so too...

... in intracellular pH (pHi) that was dependent on Ca 2+ influx from the extracellular medium as well as Ca 2+ release from intracellular pools. Surprisingly, this increase in pHi and intracellular Ca 2+ concentration,[Ca 2+ ] i , was not accompanied by any change in proton secretion. By contrast, removal...

...Carsten Hille; Bernd Walz SUMMARY Cells of the dopaminergically innervated salivary ducts in the cockroach Periplaneta americana have a vacuolar-type H + -ATPase(V-ATPase) of unknown function in their apical membrane. We have studied whether dopamine affects intracellular pH (pH i ) in duct cells...

...Khaled H. Ahmed; Bernd Pelster; Gerhard Krumschnabel SUMMARY In trout hepatocytes, hypertonicity and cytosolic acidification are known to stimulate Na + /H + exchanger (NHE) activity, which contributes to recovery of cell volume and intracellular pH (pHi),respectively. The present study...

... increase in lumen pH to 6.32±0.04 ( N =5). Intracellular pH was 6.97±0.01 and 6.82±0.04 in unstimulated and serotonin-stimulated tubules, respectively. Lumen pH was altered whereas intracellular pH was tightly regulated during serotonin and bumetanide treatment. Furthermore, DIDS or amiloride treatment did...

... are likely to result in sperm selection before the spermatozoa meet the oocytes. * Author for correspondence (e-mail: bill.holt@ioz.ac.uk ) 31 1 2006 © The Company of Biologists Limited 2006 2006 bicarbonate sperm motility sperm transport intracellular pH fallopian tube...

...Ayako Nakajima; Masaya Morita; Akihiro Takemura; Shinji Kamimura; Makoto Okuno SUMMARY Increased intracellular pH ([pH] i ) activates dynein in sea urchin and mammalian sperm and induces activation of flagellar motility. It is thought that cAMP-dependent protein phosphorylation is associated...

..., and a recovery only limited by the physical stability of the utilized optical system. Intracellular pH (pH i ) in Danio rerio embryos between 1k-cells stage and the end of epiboly was found to be well regulated to a mean value of 7.55±0.13 (± s . d .), a range distinctly more alkaline than typical values...

...-mail: jcovi1@lsu.edu ) 11 5 2005 © The Company of Biologists Limited 2005 2005 vacuolar-type ATPase CCCP bafilomycin A 1 quiescence gradient dissipation anoxia acidification intracellular pH 31 P-NMR For more than 20 years, the source of the impressive intracellular...

... downregulation, characterized by a steep drop in intracellular pH (pH i ) and ATP levels. Using dot blots of total mitochondrial RNA, we show that during in organello incubations both O 2 deprivation and acidic pH (pH 6.4) elicit increases in half-lives of selected mitochondrial transcripts on the order of five...

... ratios in non-perfused preparations of body wall musculature at various levels of extra- and intracellular pH, P CO 2 and [HCO 3 - ]. A decrease in extracellular pH from control level (7.9) to 6.7 caused a reduction in aerobic metabolic rate of both normocapnic and hypercapnic tissues by 40-45 %. O/N...

...Gerhard Krumschnabel; Claudia Manzl; Pablo J. Schwarzbaum SUMMARY Mechanisms of intracellular pH (pHi) regulation were investigated in anoxia-tolerant hepatocytes from goldfish Carassius auratus , and compared to the situation in the anoxia-intolerant hepatocytes from trout Oncorhynchus mykiss...

...-acclimated eelpout. SMR increased more strongly with rising temperature in P. brachycephalum than in Z. viviparus , which is reflected in a higher Arrhenius activation energy for oxygen consumption (99±5 kJ mol −1 , versus 55±3 kJ mol −1 for cold-acclimated Z. viviparus ; means ± S.D.). The intracellular pH...

...Basile Michaelidis; Evangelia Rofalikou; Manfred K. Grieshaber ABSTRACT The effects of hypercapnia, together with low and high levels of extracellular Ca 2+ , on heart activity and intracellular pH were examined in isolated perfused hearts from the land snail Helix lucorum . In addition...