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Keywords: immunohistochemistry
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Journal Articles
J Exp Biol (2018) 221 (9): jeb166538.
Published: 10 May 2018
... with immunohistochemistry and stereology. A pattern of UCP1 and PGC1α immunoexpressions served for estimation of the thermogenic potential of brown adipocytes, whereas immunodetection of noradrenaline allowed us to establish a correlation between morphological changes observed in BAT and noradrenergic stimulatory pathways...
Journal Articles
J Exp Biol (2015) 218 (23): 3746–3753.
Published: 1 December 2015
...-fertilization, SNP increased ventilation in a concentration-dependent manner. Inhibition of NOS activity with l -NAME or knockdown of nNOS inhibited the hypoxic ( P O 2 =3.5 kPa) ventilatory response. Immunohistochemistry revealed the presence of nNOS in the NECs of larvae. Taken together, these data suggest...
Includes: Supplementary data
Journal Articles
J Exp Biol (2015) 218 (14): 2166–2173.
Published: 1 July 2015
... that in humans. In the present study it was tested whether this is attributable to differences in muscle contractile properties. In biopsies of marmoset m. vastus lateralis (VL) and m. gastrocnemius medialis (GM) ( N =4), fibre-type distribution was assessed using fluorescent immunohistochemistry. In single...
Journal Articles
J Exp Biol (2012) 215 (12): 2088–2095.
Published: 15 June 2012
...) in position 3 and methionine (M) in position 8. Reverse transcriptase (RT)-PCR shows that Rhopr-MS is expressed in the CNS and posterior midgut in R. prolixus and immunohistochemistry suggests that an RFamide-like peptide is present in endocrine-like cells in the midgut. Physiological assays using Rhopr-MS...
Journal Articles
J Exp Biol (2011) 214 (10): 1773–1782.
Published: 15 May 2011
... is monitoring for changes in their phototactic behavior. In most arthropods, histamine has been shown to play a key role in the visual system. Currently, nothing is known about histaminergic signaling in either D. magna or D. pulex . Here, a combination of immunohistochemistry and genome mining was used...
Journal Articles
J Exp Biol (2010) 213 (14): 2536–2546.
Published: 15 July 2010
... segments (Figs 4 and 5 ) and by darkfield microscopy of the intact organ ( Fig. 6 ). * These authors contributed equally to this work † Author for correspondence ( fsmith@dal.ca ) 6 4 2010 © 2010. 2010 buoyancy sympathetic nervous system immunohistochemistry force...
Journal Articles
J Exp Biol (2010) 213 (2): 288–294.
Published: 15 January 2010
... of the aquatic anuran Xenopus laevis (Pipidae) . J. Exp. Biol. 72 , 285 - 289 . * Author for correspondence ( sbstana@ipc.shizuoka.ac.jp ) 6 10 2009 © 2010. aquaporin hydrosmotic response vasotocin hydrin β-adrenergic effectors ventral pelvic skin immunohistochemistry...
Journal Articles
J Exp Biol (2009) 212 (24): 4027–4032.
Published: 15 December 2009
... in learning change in synaptic and biochemical composition with increased foraging duration. First, we used synapse-specific immunohistochemistry and proteomics to screen for alterations in the calyx region of the mushroom body, a key structure for memory formation. Using proteomics, we next profiled...
Includes: Supplementary data
Journal Articles
J Exp Biol (2007) 210 (4): 699–714.
Published: 15 February 2007
... endocrine cells in the midgut epithelium of the crab Cancer magister ( Mykles, 1979 ). In the years that have followed, these cells have been largely ignored and nothing is known about their hormone content or the functions they play in this species. Here,we used a combination of immunohistochemistry...
Journal Articles
J Exp Biol (2006) 209 (16): 3241–3256.
Published: 15 August 2006
... hyperglycemic hormone (CHH) crustacean hyperglycemic hormone precursor-related peptide (CPRP) mandibular organ-inhibiting hormone (MOIH) moult-inhibiting hormone (MIH) immunohistochemistry Research on crustaceans has played a major role in advancing our understanding of neuroendocrine structure...
Journal Articles
J Exp Biol (2006) 209 (5): 907–915.
Published: 1 March 2006
...-mail: jeanpaul.paluzzi@utoronto.ca ) 5 1 2006 © The Company of Biologists Limited 2006 2006 anti-diuresis CAPA CAP2b neuropeptide neurosecretory cells immunohistochemistry insect Malpighian tubule Rhodnius prolixus In the haematophagous insect Rhodnius prolixus...
Journal Articles
J Exp Biol (2005) 208 (22): 4243–4253.
Published: 15 November 2005
... and two extraocular muscles (rectus lateralis and retractor bulbi) were sampled in adult male Bos taurus (age 18-24 months, mass 400-500 kg) and analysed by RT-PCR, gel electrophoresis and immunohistochemistry. Transcripts and proteins corresponding to eight MHC isoforms were identified: MHC-αand MHC-β...
Journal Articles
J Exp Biol (2004) 207 (19): 3339–3348.
Published: 1 September 2004
... secondary vessel interarterial anastomoses immunohistochemistry control structure organisation long-finned eel Anguilla reinhardtii The presence of a secondary vascular system in a number of fishes has been well documented (Vogel, 1981 , 1985a , b ; Vogel and Claviez, 1981 ; Steffensen...
Journal Articles
J Exp Biol (2004) 207 (9): 1479–1486.
Published: 1 April 2004
... the protein in the Papilio eye by light and electron microscopic immunohistochemistry using a monospecific antiserum produced against artificially expressed Papilio RBP. We found strong immunoreactivity in the primary as well as secondary pigment cells and in the tracheal cells. The pigment cells have long...
Journal Articles
J Exp Biol (2003) 206 (22): 4105–4111.
Published: 15 November 2003
... harbor seal Phoca vitulina diving skeletal muscle muscle fiber pinniped fiber type immunohistochemistry The skeletal muscles of marine mammals are well adapted for maintaining aerobic metabolism under the hypoxic conditions that occur during diving. In previous studies, investigators...
Journal Articles
J Exp Biol (2003) 206 (13): 2211–2219.
Published: 1 July 2003
... awarded to H.W. from the Deutsche Forschungsgemeinschaft. We also thank Mary Lou Norman for her help preparing paraffin sections and Patricia Fisher and Alexander Nikitin for their expertise in immunohistochemistry and imaging. References Aronson, P. S. and Giebisch, G. ( 1997 ). Mechanisms...
Journal Articles
J Exp Biol (2003) 206 (13): 2273–2280.
Published: 1 July 2003
... 2003 temperature acclimation osmoregulation Na + /K + -ATPase cortisol prolactin chloride cell immunohistochemistry real-time RT-PCR common carp Cyprinus carpio The common carp Cyprinus carpio L. is a stenohaline freshwater species. Carp originated in the Danube basin...
Journal Articles
J Exp Biol (2002) 205 (24): 3857–3876.
Published: 15 December 2002
... fluid and embryonic mass, was evident. Embryonic growth was significantly decreased, which might in part be attributed to disturbances of the maternal—foetal trophic relationship. Marked inductions of Vtg mRNA and Vtg protein, determined by RT-PCR and immunohistochemistry, respectively, were found...
Journal Articles
J Exp Biol (2002) 205 (23): 3651–3659.
Published: 1 December 2002
... descriptions. Ganglia were then processed for GABA immunohistochemistry. Fifteen of the 17 nonspiking interneurons studied were immunopositive for GABA, but two were not. The results suggest that the majority of these interneurons might exert their well-characterized effects on other neurons through...
Journal Articles
J Exp Biol (2002) 205 (17): 2605–2613.
Published: 1 September 2002
.... * Author for correspondence (e-mail: donlyc@agr.gc.ca ) 6 6 2002 © The Company of Biologists Limited 2002 2002 glutamate transporter Trichoplusia ni insect anti-peptide antibody immunohistochemistry glutamate Glutamate functions as a neurotransmitter in animals ranging...