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Leah M. Costello, Daniel García-Párraga, Jose Luis Crespo-Picazo, Jonathan R. Codd, Holly A. Shiels, William Joyce
J Exp Biol (2022) 225 (20): jeb244864.
Published: 24 October 2022
... in Testudines, we studied the hearts of loggerhead sea turtles, Caretta caretta ( n =7), using immunohistochemistry and histology. Surprisingly, we found no evidence of prominent atrial smooth muscle in C. caretta . However, smooth muscle was readily identified in the sinus venosus. Our results suggest...
Includes: Supplementary data
J Exp Biol (2018) 221 (9): jeb166538.
Published: 10 May 2018
.... Brown adipose tissue Rat Sucrose overfeeding Immunohistochemistry Ultrastructure Traditionally, the brown adipose tissue (BAT) has been recognised as a highly specialised heat-producing organ in mammals of small body mass, including human newborns ( Himms-Hagen, 1986 ; Nechad, 1986...
Cosima S. Porteus, Jacob Pollack, Velislava Tzaneva, Raymond W. M. Kwong, Yusuke Kumai, Sara J. Abdallah, Giacomo Zaccone, Eugenia Rita Lauriano, William K. Milsom, Steve F. Perry
J Exp Biol (2015) 218 (23): 3746–3753.
Published: 1 December 2015
...-fertilization, SNP increased ventilation in a concentration-dependent manner. Inhibition of NOS activity with l -NAME or knockdown of nNOS inhibited the hypoxic ( P O 2 =3.5 kPa) ventilatory response. Immunohistochemistry revealed the presence of nNOS in the NECs of larvae. Taken together, these data suggest...
Includes: Supplementary data
Rogier L. C. Plas, Hans Degens, J. Peter Meijer, Gerard M. J. de Wit, Ingrid H. C. H. M. Philippens, Maarten F. Bobbert, Richard T. Jaspers
J Exp Biol (2015) 218 (14): 2166–2173.
Published: 1 July 2015
... that in humans. In the present study it was tested whether this is attributable to differences in muscle contractile properties. In biopsies of marmoset m. vastus lateralis (VL) and m. gastrocnemius medialis (GM) ( N =4), fibre-type distribution was assessed using fluorescent immunohistochemistry. In single...
J Exp Biol (2012) 215 (12): 2088–2095.
Published: 15 June 2012
...) in position 3 and methionine (M) in position 8. Reverse transcriptase (RT)-PCR shows that Rhopr-MS is expressed in the CNS and posterior midgut in R. prolixus and immunohistochemistry suggests that an RFamide-like peptide is present in endocrine-like cells in the midgut. Physiological assays using Rhopr-MS...
J Exp Biol (2011) 214 (10): 1773–1782.
Published: 15 May 2011
... is monitoring for changes in their phototactic behavior. In most arthropods, histamine has been shown to play a key role in the visual system. Currently, nothing is known about histaminergic signaling in either D. magna or D. pulex . Here, a combination of immunohistochemistry and genome mining was used...
J Exp Biol (2010) 213 (14): 2536–2546.
Published: 15 July 2010
... chamber and is under the control of the sympathetic limb of the autonomic nervous system. * These authors contributed equally to this work † Author for correspondence ( firstname.lastname@example.org ) 6 4 2010 © 2010. 2010 buoyancy sympathetic nervous system immunohistochemistry force...
J Exp Biol (2010) 213 (2): 288–294.
Published: 15 January 2010
... immunohistochemistry the tree frog Hyla japonica Anuran amphibians are adapted to living in a diverse range of water environments. A general classification of anurans according to habitat distinguishes four groups, i.e. aquatic, semi-aquatic, terrestrial and arboreal species ( Hillman et al., 2009...
J Exp Biol (2009) 212 (24): 4027–4032.
Published: 15 December 2009
... in learning change in synaptic and biochemical composition with increased foraging duration. First, we used synapse-specific immunohistochemistry and proteomics to screen for alterations in the calyx region of the mushroom body, a key structure for memory formation. Using proteomics, we next profiled...
Includes: Supplementary data
Andrew E. Christie, Kimberly K. Kutz-Naber, Elizabeth A. Stemmler, Alexandra Klein, Daniel I. Messinger, Christopher C. Goiney, Anna J. Conterato, Emily A. Bruns, Yun-Wei A. Hsu, Lingjun Li, Patsy S. Dickinson
J Exp Biol (2007) 210 (4): 699–714.
Published: 15 February 2007
... endocrine cells in the midgut epithelium of the crab Cancer magister ( Mykles, 1979 ). In the years that have followed, these cells have been largely ignored and nothing is known about their hormone content or the functions they play in this species. Here,we used a combination of immunohistochemistry...
Yun-Wei A. Hsu, Daniel I. Messinger, J. Sook Chung, Simon G. Webster, Horacio O. de la Iglesia, Andrew E. Christie
J Exp Biol (2006) 209 (16): 3241–3256.
Published: 15 August 2006
... hormone (CHH) crustacean hyperglycemic hormone precursor-related peptide (CPRP) mandibular organ-inhibiting hormone (MOIH) moult-inhibiting hormone (MIH) immunohistochemistry Research on crustaceans has played a major role in advancing our understanding of neuroendocrine structure and control...
J Exp Biol (2006) 209 (5): 907–915.
Published: 1 March 2006
...-mail: email@example.com ) 5 1 2006 © The Company of Biologists Limited 2006 2006 anti-diuresis CAPA CAP2b neuropeptide neurosecretory cells immunohistochemistry insect Malpighian tubule Rhodnius prolixus In the haematophagous insect Rhodnius prolixus tubule fluid...
J Exp Biol (2005) 208 (22): 4243–4253.
Published: 15 November 2005
... and two extraocular muscles (rectus lateralis and retractor bulbi) were sampled in adult male Bos taurus (age 18-24 months, mass 400-500 kg) and analysed by RT-PCR, gel electrophoresis and immunohistochemistry. Transcripts and proteins corresponding to eight MHC isoforms were identified: MHC-αand MHC-β...
J Exp Biol (2004) 207 (19): 3339–3348.
Published: 1 September 2004
... vessel interarterial anastomoses immunohistochemistry control structure organisation long-finned eel Anguilla reinhardtii The presence of a secondary vascular system in a number of fishes has been well documented (Vogel, 1981 , 1985a , b ; Vogel and Claviez, 1981 ; Steffensen et al...
J Exp Biol (2004) 207 (9): 1479–1486.
Published: 1 April 2004
... the protein in the Papilio eye by light and electron microscopic immunohistochemistry using a monospecific antiserum produced against artificially expressed Papilio RBP. We found strong immunoreactivity in the primary as well as secondary pigment cells and in the tracheal cells. The pigment cells have long...
J Exp Biol (2003) 206 (22): 4105–4111.
Published: 15 November 2003
... with the enzymatic analyses of pinniped skeletal muscle that support the use of lipid-derived aerobic catabolism to fuel working muscle during diving in these marine mammals. harbor seal Phoca vitulina diving skeletal muscle muscle fiber pinniped fiber type immunohistochemistry The skeletal...
J Exp Biol (2003) 206 (13): 2211–2219.
Published: 1 July 2003
...-mail: firstname.lastname@example.org ) 17 3 2003 © The Company of Biologists Limited 2003 2003 V-type H + -ATPase Na + /K + -ATPase Malpighian tubule electrolyte secretion Na + /H + exchange polyclonal antibody immunohistochemistry western blot The V-type H + -ATPase was first discovered...
J Exp Biol (2003) 206 (13): 2273–2280.
Published: 1 July 2003
... immunohistochemistry real-time RT-PCR common carp Cyprinus carpio The common carp Cyprinus carpio L. is a stenohaline freshwater species. Carp originated in the Danube basin and their huge ability to adapt allowed them to extend their habitats throughout the temperate zone of the northern hemisphere...
Tina H. Rasmussen, Thomas K. Andreassen, Søren N. Pedersen, Leo T. M. Van der Ven, Poul Bjerregaard, Bodil Korsgaard
J Exp Biol (2002) 205 (24): 3857–3876.
Published: 15 December 2002
... fluid and embryonic mass, was evident. Embryonic growth was significantly decreased, which might in part be attributed to disturbances of the maternal—foetal trophic relationship. Marked inductions of Vtg mRNA and Vtg protein, determined by RT-PCR and immunohistochemistry, respectively, were found...
J Exp Biol (2002) 205 (23): 3651–3659.
Published: 1 December 2002
... descriptions. Ganglia were then processed for GABA immunohistochemistry. Fifteen of the 17 nonspiking interneurons studied were immunopositive for GABA, but two were not. The results suggest that the majority of these interneurons might exert their well-characterized effects on other neurons through...