.... galloprovincialis and M. trossulus to an acute heat shock in the presence and absence of the sirtuin inhibitor suramin (SIRT1, 2 and 5) showed that sirtuins affected molecular chaperones, oxidative stress proteins, metabolic enzymes, cytoskeletal and signaling proteins more in the heat-sensitive M. trossulus than...

... clear. We measured the effects of single heat shocks and of diurnally fluctuating or constant rearing temperatures on the critical thermal maximum (CT max ) for final instar larvae of Manduca sexta . Brief (2 h) heat shocks at temperatures of 35°C and above significantly increased CT max relative...

... and trehalose accumulation induced by heat shock in yeast cells of Candida albicans . FEMS Microbiol. Lett. 146 , 65 - 71 . Baker C. R. , Booth L. N. , Sorrells T. R. , Johnson A. D. ( 2012 ). Protein modularity, cooperative binding, and hybrid regulatory states underlie...

... levels of knockdown resistance to high temperature in young flies were used as the mapping population. There was no apparent increase in heat-shock survival between heat-pretreated and non-pretreated larvae. There was a positive correlation between the two experimental conditions of heat-shock survival...

... in so-called ramping assays. We exposed flies to ramping at rates of 0.06 and 0.1°C min -1 , respectively. Flies were sampled from the two treatments at 28, 30, 32, 34, 36 and 38°C and tested for heat tolerance and expression levels of the heat shock genes hsp23 and hsp70 , as well as Hsp70 protein...

... males. In the present study, real-time PCR and double-stranded RNA (dsRNA) methods were used to explore the role of heat shock protein (Hsp) genes in whitefly of both sexes; this provided further evidence of the mechanism underlying the differential heat tolerance abilities of females and males...

... by photoperiod, prior heat experience and the sex of the animal. We compared thermosensitivity and thermotolerance of ventilatory motor pattern generation in locusts reared under two photoperiods (12:12 and 16:8; i.e. 12 h:12 h and 16 h:8 h L:D, respectively) before and after heat shock pre-treatment (HS: 3 h...

... acclimation changes DNA-binding activity of heat shock factor 1 (HSF1)in the goby Gillichthys mirabilis : implications for plasticity in the heat-shock response in natural populations. J. Exp. Biol. 205 , 3231 -3240. Buckley, B. A. and Hofmann, G. E. ( 2004 ). Magnitude and duration of thermal stress...

...Ariel Shabtay; Zeev Arad SUMMARY Living organisms respond to heat exposure by selectively expressing heat shock proteins (HSPs). Accumulation of HSPs confers thermotolerance in cell cultures and in ectotherms and is an important component of the heat shock response. This response, however, has...

... ) 9 3 2004 © The Company of Biologists Limited 2004 2004 CT max temperature heat shock thermal stress Pogonomyrmex thermolimit respirometry The upper temperature at which an animal loses muscular control is a metric of interest to ecological and evolutionary biologists...

... of maintenance at 15°C of Expt 2 (24 h), Expt 3 (first 5 h) and Expt 4 (first 21 h),which represents a total of 33 observations. This survey of periods at 15°C allowed us to set the threshold values for considering significant behavior during the heat-shock experiments. Throughout the 24 h of Expt 2, the shrimps...

... for correspondence (e-mail: buckl@zoo.utoronto.ca ) 28 10 2002 © The Company of Biologists Limited 2003 2003 stress proteins forced dive recovery heat shock western blot Chrysemys picta belli western painted turtle Although vertebrates are generally very sensitive to oxygen...

... strains at different temperatures, (ii) shifting the temperature after metamorphosis and (iii)inducing a single heat-shock pulse in control and heat-sensitive transgenic strains, over a period of 3 days following adult eclosion. This study describes the time course of the events involved in the production...

...S. C. Lakhotia; K. V. Prasanth SUMMARY The haploid genome of Drosophila melanogaster normally carries at least five nearly identical copies of heat-shock-inducible hsp70 genes, two copies at the 87A7 and three copies at the 87C1 chromosome sites. We used in situ hybridization of the cDNA, which...

...Bradley A. Buckley; Marie-Eve Owen; Gretchen E. Hofmann SUMMARY Spatio-temporal variation in heat-shock gene expression gives organisms the ability to respond to changing thermal environments. The temperature at which heat-shock genes are induced, the threshold induction temperature, varies...

... purified from G. mirabilis white muscle. Similar to other chaperones in the 70kDa heat-shock protein family, G. mirabilis Hsc70 exhibited a low intrinsic ATPase activity that was stimulated in vitro by the addition of unfolded protein. Across the environmentally relevant temperature range (10–35°C...

...Julia K. Willsie; James S. Clegg SUMMARY The role of the small heat shock/α-crystallin protein, p26, in transcription in Artemia franciscana embryos was examined using isolated nuclei, containing either control or elevated levels of p26, in transcription run-on assays. Heat shock or anoxia in vivo...