... levels of haemocyanin exhibited by tidal crabs allowed them to maintain oxygen transport and buffer pH changes during emersion . This suggests that acclimation of C. maenas to submerged conditions results in a loss of important physiological mechanisms that enable it to tolerate emersion. The results...

... characteristic absorbance spectra for haemolymph containing haemocyanin with an oxygenation-dependent peak at 347 nm ( O. vulgaris ; Fig. 1A ), and multiple responsive peaks at 540, 575, 412 and 335 nm for oxygenated haemoglobin-bearing blood and at 553, 427 and 366 nm for deoxygenated haemoglobin-bearing blood...

...Jane W. Behrens; John P. Elias; H. Harry Taylor; Roy E. Weber SUMMARY We investigated divalent cation and anaerobic end-product concentrations and the interactive effects of these substances and pH on haemocyanin oxygen-binding (Hc-O 2 ) in the New Zealand abalone Haliotis iris . During 24 h...

...Michael A. Menze; Nadja Hellmann; Heinz Decker; Manfred K. Grieshaber ABSTRACT Haemocyanin serves as an oxygen carrier in the haemolymph of decapod crustaceans. The oxygen-binding behaviour of the pigment is modulated by the two major anaerobic metabolites, L-lactate and urate. The binding...

...Agnieszka M. Adamczewska; Stephen Morris ABSTRACT Gecarcoidea natalis is a land crab that migrates annually several kilometres to breed. The O 2 -binding properties of haemocyanin in G. natalis were investigated in vitro to test the idea that the O 2 -binding properties of the haemocyanin of land...

... periods of up to 45 min. During this exercise period, blood gas measurements were made on venous, pulmonary and arterial samples to assess the function of the lungs in gas exchange and the performance of the circulatory system in gas transport and to determine the role and importance of the haemocyanin...

...) experiments. In previous reports on O2 equilibrium curves of E. californicum haemocyanin, Tris–HCl buffer was used to set the pH, whereas in our experiments, pH was adjusted by altering the partial pressure of CO 2 . The O2 concentration measurements demonstrate an increase in oxygen affinity of E...

...C. Agnisola; D. F. Houlihan ABSTRACT The effect of increasing oxygen supply on the perfused systemic heart of Octopus vulgaris (Lam.) by using oxygenated or haemocyanin-containing perfusates was investigated. Providing aerated blood or seawater solutions of haemocyanin that were comparable...

...N. K. Sanders; J. J. Childress ABSTRACT The effects of temperature and pH on haemocyanin oxygen-binding were compared for three species of diurnally vertically migrating and two species of non-migrating, pelagic oplophorid shrimps from the deep sea off the Hawaiian island of Oahu. The effects of L...

..., Italy. ‡ Present address: Department of Biochemistry, University of Chieti, 31100 Chieti, Italy. 28 11 1989 © 1990 by Company of Biologists 1990 gastropods haemocyanin oxygen binding temperature tolerance It has been established that inorganic ions...

...F. Lallier; J. P. Truchot ABSTRACT The addition of either L-lactate or urate to dialysed haemolymph from the prawn Penaeus japonicus (Bate) increased the in vitro haemocyanin oxygen affinity. The quantitative values of these two effects, expressed as ΔlogP 50 / Δlog[effector], were found to be −0...

... of temperature on haemocyanin oxygen-affinity was pronounced (ΔH = –39 kJ mol −1 ) and was considered to represent the absence of any specific adaptation to environmental temperature. The Bohr effect was large for a terrestrial decapod ( ϕ = –0·60), although reduced at low pH. Changes in [Ca] had a significant...

... crabs (43·8 mmHg; 1 mmHg = 133·3 Pa) but fell during exercise (to 27 mmHg). remained constant at 10–12 mmHg. rose substantially during exercise (from 7·1 to 14·6mmHg). Haemocyanin delivered 90% of oxygen in resting crabs rising to 97% following exercise. Oxygen delivery at rest was 0·46 mmol l −1...

...S. Morris 14 4 1988 © 1988 by Company of Biologists 1988 haemocyanin freezing oxygen affinity association state The addition of protease inhibitors and antibiotics provides a valuable means of stabilizing proteins in solution but this is less desirable...

...S. Morris; C. R. Bridges; M. K. Grieshaber ABSTRACT The binding of Ca 2+ to the haemocyanin of the crayfish Austropotamobius pallipes was investigated. The amount of bound Ca 2+ was determined using an ultrafiltration technique to produce haemocyanin-free solutions, the Ca 2+ concentration of which...

...+ on haemocyanin oxygen affinity were found to be interdependent. Elevating the concentration of one of these two ions reduced the effect of the other. The increase in the oxygen affinity of the haemocyanin brought about by elevated Ca 2+ and L-lactate was insufficient to account for the difference in affinity...

... respectively. Changes in the concentrations of these ions could also be correlated with changes in haemocyanin oxygen affinity. Reimmersion and recovery returned all parameters to near control values. The effect, on haemocyanin oxygen affinity, of the Bohr shift alone was calculated and compared...

...S. Morris; C. R. Bridges ABSTRACT The oxygen affinity of the haemocyanin in the supralittoral crab Ocypode saratan was investigated at temperatures between 20 and 35 °C. The effect of L-lactate on dialysed and undialysed haemolymph oxygen affinity was also examined. In general, the temperature...