... and two extraocular muscles (rectus lateralis and retractor bulbi) were sampled in adult male Bos taurus (age 18-24 months, mass 400-500 kg) and analysed by RT-PCR, gel electrophoresis and immunohistochemistry. Transcripts and proteins corresponding to eight MHC isoforms were identified: MHC-αand MHC-β...

... of regulation. © The Company of Biologists Limited 2004 2004 As already described ( Toursel et al.,2000 ), the MHC composition was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on a 4.5% stacking gel and on a 7.5% separating gel. Electrophoresis was for 20 h at 12°C...

... of secretion;however, the amino-terminal sequences for both were found in protein bands of one-dimensional electrophoresis of saliva samples (see below). Finally, ISL939_cluster238 has very high similarity to Drosophila melanogaster NADH-ubiquinone oxidoreductase, a typical mitochondrial enzyme ranging...

... muscle electrophoresis contractile protein isoform. The motor protein myosin in skeletal muscle is a hexameric polypeptide consisting of two myosin heavy chain (MHC) and four myosin light chain (MLC) subunits. Each MHC is associated with one essential light chain (ELC) and one regulatory light...