...Kazuyuki Sugino; Takashi Tominaga; Richard D. Allen; Yutaka Naitoh SUMMARY The contractile vacuole complex of Paramecium multimicronucleatum transforms into membrane-bound vesicles on excision from the cell. The I – V relationship was linear in a voltage range of–80 to +80 mV in all vesicles...

...Masaaki Iwamoto; Richard D. Allen; Yutaka Naitoh SUMMARY The freshwater ciliated protozoan, Paramecium multimicronucleatum ,usually possesses two contractile vacuole complexes (CVCs). The number of CVCs in a single cell, however, may vary from 1 to 7. We found that the number of cells that have...

.... The current generated by a single arm was approximately 1.3×10 -10 A. When cells adapted to a hypotonic solution were exposed to a hypertonic solution, the rate of fluid segregation, R CVC , in the contractile vacuole complex (CVC) diminished at the same time as immunological labelling for V-ATPase...

...Takashi Tominaga; Richard D. Allen; Yutaka Naitoh ABSTRACT The freshwater protozoan Paramecium multimicronucleatum maintains a constant cytosolic osmolarity through the exocytotic activity of its contractile vacuole complex. The contractile vacuole (CV) expels the excess cytosolic water, acquired...

...Takashi Tominaga; Richard D. Allen; Yutaka Naitoh ABSTRACT In the freshwater protozoan Paramecium multomicronucleatum , excess cytosolic water, acquired osmotically, is segregated and expelled to the cell exterior through the activity of the contractile vacuole complex. This process keeps the cell...