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Keywords: cloning
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Journal Articles
J Exp Biol (2009) 212 (2): 249–256.
Published: 15 January 2009
... putatively encoding a 403-residue protein, the turtle urea transporter (turtle UT). The first cloned reptilian UT has high homology with UTs (facilitated urea transporters) cloned from vertebrates, and most closely resembles the UT-A subfamily. Injection of turtle UT cRNA into Xenopus oocytes induced a 6...
Journal Articles
J Exp Biol (2008) 211 (1): 92–105.
Published: 1 January 2008
... generated full-length constructs using standard recombinant techniques, as described above. The 5-HT 1α receptors were first cloned into pIRESneo and stably transfected into several cell lines using lipofectamine as detailed in the Results. Immunoblotting experiments coupled with the lack of growth after...
Journal Articles
J Exp Biol (2005) 208 (16): 3177–3197.
Published: 15 August 2005
...H.-W. Kim; E. S. Chang; D. L. Mykles SUMMARY Crustacean muscle has four calpain-like proteinase activities (CDP I, IIa,IIb and III) that are involved in molt-induced claw muscle atrophy, as they degrade myofibrillar proteins in vitro and in situ . Using PCR cloning techniques, three full-length...
Journal Articles
J Exp Biol (2004) 207 (10): 1625–1632.
Published: 15 April 2004
... electron microscopic studies. Generally receptor sequestration follows its desensitization, which is initiated by receptor phosphorylation by G-protein-coupled receptor kinase. We cloned and sequenced the gene fragment of 407 nucleotides homologous to theβ-adrenergic receptor kinase (βARK): its deduced...
Journal Articles
J Exp Biol (2002) 205 (5): 575–584.
Published: 1 March 2002
... of Biologists Limited 2002 2002 Na + channel cloning phylogeny evolution diversity. Voltage-gated Na + channels play a critical role in controlling the electrical excitability of animal cells, being primarily responsible for the rising phase of the action potential. The electrophysiological...
Journal Articles
J Exp Biol (2001) 204 (14): 2517–2525.
Published: 15 July 2001
.... Holmqvist, W. J. DeGrip, J. V. Helvik and P. Ekström, in preparation). ‡Author for correspondence at address 3 (e-mail: bo.holmqvist@pat.lu.se ) 19 4 2001 © The Company of Biologists Limited 2001 2001 pineal organ retina fish cloning mRNA in situ hybridisation...