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J Exp Biol (2009) 212 (2): 249–256.
Published: 15 January 2009
... putatively encoding a 403-residue protein, the turtle urea transporter (turtle UT). The first cloned reptilian UT has high homology with UTs (facilitated urea transporters) cloned from vertebrates, and most closely resembles the UT-A subfamily. Injection of turtle UT cRNA into Xenopus oocytes induced a 6...
J Exp Biol (2008) 211 (1): 92–105.
Published: 1 January 2008
... generated full-length constructs using standard recombinant techniques, as described above. The 5-HT 1α receptors were first cloned into pIRESneo and stably transfected into several cell lines using lipofectamine as detailed in the Results. Immunoblotting experiments coupled with the lack of growth after...
J Exp Biol (2005) 208 (16): 3177–3197.
Published: 15 August 2005
...H.-W. Kim; E. S. Chang; D. L. Mykles SUMMARY Crustacean muscle has four calpain-like proteinase activities (CDP I, IIa,IIb and III) that are involved in molt-induced claw muscle atrophy, as they degrade myofibrillar proteins in vitro and in situ . Using PCR cloning techniques, three full-length...
J Exp Biol (2004) 207 (10): 1625–1632.
Published: 15 April 2004
... electron microscopic studies. Generally receptor sequestration follows its desensitization, which is initiated by receptor phosphorylation by G-protein-coupled receptor kinase. We cloned and sequenced the gene fragment of 407 nucleotides homologous to theβ-adrenergic receptor kinase (βARK): its deduced...
J Exp Biol (2002) 205 (5): 575–584.
Published: 1 March 2002
... of Biologists Limited 2002 2002 Na + channel cloning phylogeny evolution diversity. Voltage-gated Na + channels play a critical role in controlling the electrical excitability of animal cells, being primarily responsible for the rising phase of the action potential. The electrophysiological...
J Exp Biol (2001) 204 (14): 2517–2525.
Published: 15 July 2001
.... Holmqvist, W. J. DeGrip, J. V. Helvik and P. Ekström, in preparation). ‡Author for correspondence at address 3 (e-mail: firstname.lastname@example.org ) 19 4 2001 © The Company of Biologists Limited 2001 2001 pineal organ retina fish cloning mRNA in situ hybridisation...