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Keywords: citrate synthase
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Journal Articles
J Exp Biol (2021) 224 (2): jeb236257.
Published: 26 January 2021
... by acute and chronic warming. Warm acclimation preserves complex I coupling, mitigates ROS production and decreases the sensitivity of mitochondrial respiration to nitric oxide. Anoxia–reoxygenation Citrate synthase Nitric oxide Reactive oxygen species Mitochondrial respiration Superoxide...
Includes: Supplementary data
Journal Articles
J Exp Biol (2019) 222 (22): jeb208074.
Published: 27 November 2019
...) composition between groups. Hypoxia acclimation did not alter mitochondrial coupled or uncoupled respiration, or respiratory control ratio, ROS release rates, P 50 or superoxide dismutase activity. However, it increased citrate synthase activity (by ∼20%), increased the sensitivity of mitochondrial...
Includes: Supplementary data
Journal Articles
J Exp Biol (2018) 221 (4): jeb171397.
Published: 20 February 2018
... addition. Protein content was analysed using the Bradford technique ( Bradford, 1976 ). Citrate synthase (CS) and ETS complex maximal activities ( V max ) were assessed using spectrophotometric biochemical assays from whole brain, as described previously ( Galli et al., 2013 ). Next we...
Journal Articles
J Exp Biol (2017) 220 (3): 386–396.
Published: 1 February 2017
... on cardiac lipid composition. In both populations of perch, the activity of glycolytic (pyruvate kinase and lactate dehydrogenase) and tricarboxylic acid cycle (pyruvate dehydrogenase and citrate synthase) enzymes increased with acute warming. However, at temperatures exceeding 30°C, a drastic thermally...
Includes: Supplementary data
Journal Articles
J Exp Biol (2015) 218 (24): 3885–3893.
Published: 1 December 2015
... levels of fatty acid translocase (FAT/CD36), cytosolic fatty acid-binding protein, and citrate synthase (CS) activity. However, mRNA expression of FAT/CD36 and both mRNA and protein expression of plasma membrane fatty acid-binding protein did not change for either training group. CS activities...
Journal Articles
J Exp Biol (2014) 217 (6): 866–875.
Published: 15 March 2014
... increased for cold-exposed, but not for warm-exposed, birds. LD birds gained more M b than SD birds, irrespective of temperature. Flight muscle size and mass did not differ significantly among groups, but heart mass was larger in cold-exposed birds. Citrate synthase, carnitine palmitoyl transferase and β...
Journal Articles
J Exp Biol (2013) 216 (15): 2889–2895.
Published: 1 August 2013
... mechanisms involved in energy production, including mitochondrial respiration at different steps of the electron transport system (ETS) and related the results to citrate synthase activity in the liver of non-reproductive and reproductive (two and eight pups) female house mice at peak lactation. Whereas we...
Journal Articles
J Exp Biol (2012) 215 (19): 3370–3378.
Published: 1 October 2012
... and mitochondrial ultrastructure of Clione antarctica from the Southern Ocean (−1.8°C) and populations of a sister species, Clione limacina , from the Arctic (−0.5 to 3°C) and from the North Atlantic (10°C). We also measured oxygen consumption and the activity of the mitochondrial enzyme citrate synthase (CS...
Journal Articles
J Exp Biol (2010) 213 (3): 445–452.
Published: 1 February 2010
...-fold in killifish, following cold acclimation, but was unchanged in cardiac muscle and also unaffected by exercise acclimation in either tissue. No changes in citrate synthase activity were detected in either tissue following temperature acclimation. Enzymatic antioxidants (catalase and superoxide...
Journal Articles
J Exp Biol (2009) 212 (6): 808–814.
Published: 15 March 2009
... animals were burdened with substantially higher metabolic loads(defined as C or digestible food intake per total mass of a particular organ). The mass-specific activity of citrate synthase (CS) in the liver and kidneys(but not heart) was also lower in the low-BMR mice. The magnitude of phenotypic...
Journal Articles
J Exp Biol (2009) 212 (4): 461–470.
Published: 15 February 2009
.... The proximal lateral rectus muscle is darker red in color and has a higher citrate synthase activity, indicating a higher capacity for aerobic heat production, than all other extraocular muscles. Furthermore,this muscle has a layer of fat insulating it from the gill cavity and is perfused by a network...
Journal Articles
J Exp Biol (2008) 211 (8): 1262–1269.
Published: 15 April 2008
...) versus warm- (11°C) exposed fish. Adenosine levels increased during cold exposure in vivo and reached a transient maximum after 24 h in serum, but remained permanently elevated in liver. Whole animal cold acclimation induced a rise of liver citrate synthase activity by 44±15%, but left cytochrome c...
Journal Articles
J Exp Biol (2007) 210 (21): 3798–3804.
Published: 1 November 2007
... activities of oxidative and glycolytic enzymes. Mass-specific activity of the oxidative enzyme citrate synthase (CS) scaled negatively with body size in each species, with scaling coefficients ranging from –0.15 to –0.19, whereas the glycolytic enzyme pyruvate kinase (PK) showed positive scaling...
Journal Articles
J Exp Biol (2007) 210 (1): 1–11.
Published: 1 January 2007
... show that scaling of mass-specific metabolism and citrate synthase activity in epipelagic squids (Loliginidae, Ommastrephidae and Gonatidae) approaches mass-independence ( b <-0.10) while metabolism in other cephalopod groups scales near a quarter-power ( Fig. 2C ). Other cephalopods are also...
Includes: Supplementary data
Journal Articles
J Exp Biol (2006) 209 (13): 2462–2471.
Published: 1 July 2006
... populations of the North Sea and the Barents Sea,respectively. In white muscle, cold acclimation caused a parallel increase in citrate synthase (CS) and in cytochrome c oxidase (COX) activities,but with a much larger effect in the cold eurythermal Arctic population. In liver, cold acclimation was accompanied...
Journal Articles
J Exp Biol (2005) 208 (9): 1601–1610.
Published: 1 May 2005
... and mtDNA genes Not measured PPARδ: Targeted overexpression (sixfold) of a native PPAR delta in mouse skeletal muscle 4 50% increase in citrate synthase and HOAD mRNA in tibialis anterior; twofold increase in fatty acid binding protein No change in mRNA for LDH and alpha glycerophosphate...
Journal Articles
J Exp Biol (2004) 207 (23): 4045–4056.
Published: 1 November 2004
... white and dark muscle. An analysis of isolated fibers would be a useful approach to address this issue. Another argument for the presence of diffusive limitations in these large white fibers can be made by examining the scaling of the activity of the aerobic enzyme citrate synthase ( Fig. 5...
Journal Articles
J Exp Biol (2004) 207 (5): 881–890.
Published: 15 February 2004
... ) 3 12 2003 © The Company of Biologists Limited 2004 2004 Pacific bluefin tuna Thunnus orientalis in situ heart preparation temperature citrate synthase Ringer's solution consisted of (in mmol l –1 ) 185.7 NaCl,1.1 MgCl 2 , 7.0 KCl, 3.22 CaCl 2 , 10 sodium pyruvate and 10...
Journal Articles
J Exp Biol (2003) 206 (16): 2845–2857.
Published: 15 August 2003
... other actively swimming shark species. The metabolic enzymes measured were citrate synthase(CS), an index of aerobic capacity, and lactate dehydrogenase (LDH), an index of anaerobic capacity. WM creatine phosphokinase (CPK) activity, an index of rapid ATP production during burst swimming, was also...
Journal Articles
J Exp Biol (2003) 206 (7): 1193–1200.
Published: 1 April 2003
...) for analysis of metabolic enzyme activity (lactate dehydrogenase, citrate synthase and cytochrome c oxidase). There were significant differences in mean daily body temperatures between winter (15.66±0.43°C; mean ± S.E.M.) and summer(29.34±0.21°C), and daily body temperatures fluctuated significantly more...