... + -permeable paracellular pathway and an increase in conductance of the epithelium. Tight junction CFTR Immunocytochemistry Electrophysiology Fundulus heteroclitus Euryhaline Teleost fish Gill epithelium Ionocyte Chloride cell The mummichog (or common killifish) Fundulus heteroclitus...

... of branchial chloride cells in killifish transferred from seawater to freshwater, revealed by morphofunctional observations and a newly established `time-differential double fluorescent staining' technique. J. Exp. Biol. 206 , 4113 -4123. Laurent, P. ( 1984 ). Gill internal morphology. In Fish...

... and SW salmon. Labelling occurred mainly in presumed chloride cells and cells deep in the filament but occasionally also on lamellae. Overall, the salinity-induced variation in labelling pattern and intensity matched the quantification data. In conclusion, the predominant switching of Na + ,K + –ATPase α...

.... ( 1999 ). In vivo sequential changes in chloride cell morphology in the yolk-sac membrane of Mozambique tilapia ( Oreochromis mossambicus )embryos and larvae during seawater adaptation. J. Exp. Biol. 202 , 3485 -3495. Hiroi, J., McCormick, S. D., Ohtani-Kaneko, R. and Kaneko,T. ( 2005...

... Mitochondria-rich cell chloride cell peanut lectin agglutinin hypercapnia cortisol gill NHE NHE2 NHE3 sodium/proton exchangers fish The PNA + MRC of trout is characterized by a tubular network arising from extensive infolding of the basolateral plasma membrane. The PNA – MRC has...

... accession no. AY679524). The mismatch or deleted residues are underlined. The conserved TSC-22 domain is bold. * Author for correspondence (e-mail: ckcwong@hkbu.edu.hk ) 31 3 2008 © The Company of Biologists Limited 2008 2008 chloride cell organic osmolyte osmosensor pavement...

...-1 Sugao, Miyamae-ku, Kawasaki 216-8511, Japan (e-mail: j-hiroi@marianna-u.ac.jp ) 17 1 2007 © The Company of Biologists Limited 2007 2007 mitochondria-rich cell chloride cell salinity tolerance Na + /K + -ATPase Na + /K + /2Cl – cotransporter salmonid lake trout brook...

... + -ATPase, which are essential for ionic regulation in seawater, and the appearance of chloride cell-accessory cell complexes,which are normally restricted to fish inhabiting seawater. These data provide compelling evidence that gill remodelling during migration from freshwater to seawater may involve...

... that an ancestral form of the mammalian genes diverged from those in the lower vertebrates prior to the gene duplication event that gave rise to TRPV5 and TRPV6. The putative model for Ca 2+ uptake in fish proposes that the mitochondria-rich cell (also termed ionocyte or chloride cell) is the predominant...

... in the gills (chloride cells) rapidly regulate NaCl secretion for systemic osmoregulation while they simultaneously are exposed to acute osmotic shock. This study was conducted to determine if COX2 is expressed in the gills, and if so, to evaluate its function in cellular and systemic osmoregulation...

... absorption is proposed based on type-II cells possessing apical NKCC. * Author for correspondence (e-mail: j-hiroi@marianna-u.ac.jp ) 16 3 2005 © The Company of Biologists Limited 2005 2005 mitochondrion-rich cell chloride cell Na + /K + -ATPase Na + /K + /2Cl - cotransporter...

...) cell, intercalated MR cell, chloride cell and pavement cell] differ in several respects from those of teleosts. The ultrastructural characteristics of these cells are distinctive and closely resemble those of certain ion-transporting epithelia in other vertebrates, for which the function has been...

...Raquel N. Lima; Dietmar Kültz SUMMARY The effects of salinity on chloride cells (CC) and Na + /K + -ATPase content in gill epithelium of euryhaline killifish Fundulus heteroclitus were analyzed using laser scanning cytometry (LSC) and tissue microarrays (TMAs). Salinity acclimations consisted...

... consumption, drinking rate, hematocrit, mean cell hemoglobin concentration, and muscle water content), biochemical(Na + , K + -ATPase) and morphological (number of mature,accessory, immature and apoptotic chloride cells) indicators of osmoregulatory stress were measured. Tilapia tolerated salinities ranging...

... collected from a comparable area in the trailing edge of the filament where the chloride cells reside. Care was taken to orient the filaments in a standardised way to obtain cross-sections through the secondary lamellae perpendicular to the axis of the filament. After dewaxing, blocking of endogenous...

...Katherine A. Sloman; Patrick R. Desforges; Kathleen M. Gilmour SUMMARY Fish acclimated to ion-deficient water exhibit proliferation of branchial chloride cells. The objective of the present study was to investigate the role of cortisol in this response using the corticosteroid receptor antagonists...

... euryhaline fish gill epithelium salinity adaptation chloride cell killifish Fundulus heteroclitus . Euryhaline teleosts maintain their plasma osmotic homeostasis by extrarenal transport of salt. In a plasma-hyperosmotic environment (e.g. sea water, SW) they actively secrete NaCl, while...

...Kiyono Shiraishi; Junya Hiroi; Toyoji Kaneko; Manabu Matsuda; Tetsuya Hirano; Takao Mori SUMMARY To examine the functional differentiation of chloride cells in the yolk-sac membrane of tilapia ( Oreochromis mossambicus ) embryos, we developed a ‘yolk-ball’ incubation system in which the yolk sac...

...Junya Hiroi; Toyoji Kaneko; Masaru Tanaka ABSTRACT Changes in chloride cell morphology were examined in the yolk-sac membrane of Mozambique tilapia ( Oreochromis mossambicus ) embryos and larvae transferred from fresh water to sea water. By labelling chloride cells with DASPEI, a fluorescent probe...

... for correspondence (e-mail: jspecker@950.uri.edu ) 14 06 1999 25 08 1999 © 1999 by Company of Biologists 1999 chloride cell mitochondria-rich cell metamorphosis osmoregulation flounder Paralichthys dentatus Summer flounder ( Paralichthys dentatus ) are teleosts...