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Keywords: carbonic anhydrase
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Journal Articles
J Exp Biol (2020) 223 (5): jeb212928.
Published: 12 March 2020
... knockdown or CRISPR/Cas9 knockout approaches were used to evaluate the roles of the two principal apical membrane acid excretory pathways, the Na + /H + exchanger (NHE3b; slc9a3.2 ) and the H + -ATPase ( atpv1aa ). Additionally, the role of HR cell cytosolic carbonic anhydrase (CAc) was investigated because...
Includes: Supplementary data
Journal Articles
J Exp Biol (2018) 221 (6): jeb151894.
Published: 21 March 2018
... for ammonia excretion and/or acid–base regulation, as supported by high expression levels of Rhesus-protein 1 (LpRh-1), cytoplasmic carbonic anhydrase (CA-2) and hyperpolarization-activated cyclic nucleotide-gated K + channel (HCN) compared with the PMPA and dorsal half-lamella. The half-lamellae displayed...
Journal Articles
J Exp Biol (2016) 219 (14): 2088–2097.
Published: 15 July 2016
.... pH Soluble adenylyl cyclase Carbonic anhydrase Carbon-concentrating mechanism Ocean acidification Osedax The vacuolar-type H + -ATPase (VHA) is a multi-subunit enzyme that is ubiquitous in eukaryotic cells. VHA uses the energy from ATP hydrolysis to drive H + transport against...
Journal Articles
J Exp Biol (2016) 219 (9): 1346–1355.
Published: 1 May 2016
... and bacterial ammonium transporters supports ammonia excretion at the anal papillae of mosquito larvae. Na + /K + -ATPase V-type H + -ATPase NHE3 Carbonic anhydrase Ammonium transporter Rhesus glycoprotein Ammonia (NH 3 /NH 4 + ) is a compound of major importance to life on earth, serving...
Journal Articles
J Exp Biol (2015) 218 (13): 2023–2029.
Published: 1 July 2015
... of Biologists Ltd 2015 Summary: Fluid absorption in the isolated midgut of adult female yellow fever mosquitoes is shown to spontaneously proceed at rates expected from in vivo urine excretion, and Na + /K + pumps, V-type H + pumps and carbonic anhydrase are shown to be important for the active...
Includes: Supplementary data
Journal Articles
J Exp Biol (2015) 218 (5): 675–683.
Published: 1 March 2015
... rates were enhanced in media buffered to pH 5 and decreased at pH 9.5. Inhibitor experiments, combined with enzyme activity measurements and mRNA expression analyses, further suggested that the excretion mechanism involves the participation of the V-type H + -ATPase, carbonic anhydrase, Na + /K...
Includes: Supplementary data
Journal Articles
J Exp Biol (2014) 217 (13): 2309–2320.
Published: 1 July 2014
... as an osmoconformer in seawater). Additionally, expression of ion transporters and supporting enzymes that typically undergo upregulation during salinity transfer in osmoregulatory gills (i.e. Na + /K + -ATPase, carbonic anhydrase, Na + /K + /2Cl − cotransporter, V-type H + -ATPase and arginine kinase) were generally...
Includes: Supplementary data
Journal Articles
J Exp Biol (2014) 217 (5): 663–672.
Published: 1 March 2014
... with carbonic anhydrases to act as a de facto sensor of pH and CO 2 . The two catalytic domains of sAC are related to HCO 3 − -regulated adenylyl cyclases from cyanobacteria, suggesting the cAMP pathway is an evolutionarily conserved mechanism for sensing CO 2 levels and/or acid/base conditions. Reports of sAC...
Journal Articles
J Exp Biol (2011) 214 (14): 2319–2328.
Published: 15 July 2011
... of the presence of a Root effect (a reduction in oxygen carrying capacity of the blood with a reduction in pH), the latter could otherwise be impaired. However, plasma-accessible carbonic anhydrase (CA) at the tissues (and absence at the gills) may result in selective short-circuiting of RBC βNHE pH regulation...
Journal Articles
J Exp Biol (2010) 213 (3): 459–468.
Published: 1 February 2010
... anion exchange SLC4 water absorption carbonic anhydrase acid–base balance During the last decade, intestinal HCO 3 – secretion via apical Cl – /HCO 3 – exchange has become recognized as an important component in the processing of seawater ingested to meet the osmoregulatory needs...
Journal Articles
J Exp Biol (2009) 212 (23): 3837–3845.
Published: 1 December 2009
...K. M. Gilmour; K. Thomas; A. J. Esbaugh; S. F. Perry SUMMARY Carbonic anhydrase (CA) is critical for CO 2 excretion in adult fish, but little is known of the expression or function of CA during early development. The present study examined the hypothesis that, as rates of CO 2 production increased...
Journal Articles
J Exp Biol (2009) 212 (11): 1662–1671.
Published: 1 June 2009
... digestive environment in the midgut. The probable principle anion influencing the highly alkaline gut lumen is bicarbonate/carbonate. Bicarbonate/carbonate is regulated at least in part by the activity of carbonic anhydrases. Hence, we have focused attention on the carbonic anhydrases of the mosquito larva...
Journal Articles
J Exp Biol (2009) 212 (11): 1647–1661.
Published: 1 June 2009
...K. M. Gilmour; S. F. Perry SUMMARY Carbonic anhydrase (CA) is the zinc metalloenzyme that catalyses the reversible reactions of CO 2 with water. CA plays a crucial role in systemic acid–base regulation in fish by providing acid–base equivalents for exchange with the environment. Unlike air...
Journal Articles
J Exp Biol (2009) 212 (11): 1731–1744.
Published: 1 June 2009
... and posterior regions. The anterior segment is probably absorptive. The middle midgut secretes acid (pH<4.0), a process dependent on a carbonic-anhydrase-catalysed H + pool. Cells of the middle segment are alternately absorptive (apically amplified by ≈9-fold, basally amplified by >90-fold) and secretory...
Journal Articles
J Exp Biol (2009) 212 (6): 878–892.
Published: 15 March 2009
... transport sodium uptake cortisol H + -ATPase carbonic anhydrase transepithelial potential Ammonia (throughout this paper, the term `ammonia' is used to refer to total NH 3 + NH 4 + , whereas these chemical symbols are used to refer to the two components) is a toxic end-product of protein...
Journal Articles
J Exp Biol (2008) 211 (19): 3067–3076.
Published: 1 October 2008
... published data exist on the recta of anopheline larvae. To gain insight into the structure and functions of this organ in anopheline species, we used immunohistochemistry to compare the localization of three proteins [carbonic anhydrase (CA9),Na + /K + P-ATPase and H + V-ATPase] in the recta of anopheline...
Journal Articles
J Exp Biol (2008) 211 (5): 805–815.
Published: 1 March 2008
... microfluometrically whether V-ATPase and carbonic anhydrase (CA) are involved in intracellular pH (pH i ) regulation,and we have localized CA activity by histochemistry. We show: (1) mean pH i in salivary gland cells is 7.5±0.3 pH units( N =96), higher than that expected from passive H + distribution; (2) low 5-HT...
Journal Articles
J Exp Biol (2007) 210 (22): 3919–3930.
Published: 15 November 2007
... for the enzyme carbonic anhydrase (CA). CA has been cloned from the alimentary canal epithelium of Anopheles gambiae larvae and can generate bicarbonate, which is implicated as a buffer for the larval lumen. The question remains as to how the bicarbonate is transported from the cells into the lumen. We...
Journal Articles
J Exp Biol (2007) 210 (13): 2320–2332.
Published: 1 July 2007
...Laetitia Serrano; Kenneth M. Halanych; Raymond P. Henry SUMMARY Two isoforms of the enzyme carbonic anhydrase (CA) in the blue crab gill,CasCAg and CasCAc, were identified, sequenced, and found to match the membrane-associated and cytoplasmic isoforms, respectively. The membrane-associated isoform...
Journal Articles
J Exp Biol (2006) 209 (20): 4040–4050.
Published: 15 October 2006
... ion absorption from food when it is present in the gut. Modulation of net ion flux occurred without changes in mRNA levels of many ion transporters (Na + /K + -ATPase α 1a ,carbonic anhydrase 2, CFTR Cl – channel,Na + /K + /2Cl – cotransporter 2, and the signalling protein 14-3-3a), and before...