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J Exp Biol (2021) 224 (12): jeb242205.
Published: 11 June 2021
...-ATPase) by binding to the cysteinyl residue on the V 1A subunit ( Bowman and Bowman, 1986 ), whereas bafilomycin binds to the V 0 subunit c ( Bowman and Bowman, 2002 ). The inhibitor 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) may also act as a sulfhydryl reagent with V-ATPase, rather than...
Includes: Supplementary data
J Exp Biol (2021) 224 (3): jeb233890.
Published: 4 February 2021
... in distilled water, D. pagei survived without mortality, hemolymph osmolality and [Cl − ] increased briefly, stabilizing at initial values, while [Na + ] decreased continually. Expression of gill V-type H + -ATPase (V-ATPase), Na + /K + -ATPase and Na + /K + /2Cl − symporter genes was unchanged. In M. jelskii...
J Exp Biol (2017) 220 (17): 3172–3180.
Published: 1 September 2017
... ). The storage of protein, fat, glycogen and uric acid in the fat body and other tissues of mosquito larvae . J. Exp. Biol. 1942 , 56 - 77 . Zhuang , Z. , Linser , P. J. and Harvey , W. R. ( 1999 ). Antibody to H + V-ATPase subunit E colocalizes with portasomes in alkaline larval midgut...
Daniel Thiel, Maja Hugenschütt, Heiko Meyer, Achim Paululat, Alex R. Quijada-Rodriguez, Günter Purschke, Dirk Weihrauch
J Exp Biol (2017) 220 (3): 425–436.
Published: 1 February 2017
... from the lack of an apical V-ATPase and the observed unaltered ammonia excretion rate when animals were exposed to an environment that was buffered to pH 6, a condition that, considering a physiological intracellular pH between 7.3 and 7.8, established a considerable outwardly directed P NH 3...
Aida Adlimoghaddam, Mélanie Boeckstaens, Anna-Maria Marini, Jason R. Treberg, Ann-Karen C. Brassinga, Dirk Weihrauch
J Exp Biol (2015) 218 (5): 675–683.
Published: 1 March 2015
... expression levels of CeRhr-1 and CeRhr-2, V-ATPase and Na + /K + -ATPase also increased significantly in response to 1 mmol l −1 NH 4 Cl. Importantly, a functional expression analysis showed, for the first time, ammonia transport capabilities for CeRhr-1 in a phylogenetically ancient invertebrate system...
Includes: Supplementary data
J Exp Biol (2014) 217 (21): 3815–3822.
Published: 1 November 2014
...-IR endocrine cells in the midge is unclear. These cells may sense nutritional content and AST may affect midgut muscle, enzyme production and/or ion transport. Further study in these areas can determine the function of AST in the endocrine cells. Na + /K + -ATPase V-ATPase Ion transport...
J Exp Biol (2014) 217 (5): 663–672.
Published: 1 March 2014
... of Biologists Ltd 2014 V-ATPase Acid/base cAMP Carbonic anhydrase pH sensing Proton pump Cyclic adenosine monophosphate (cAMP) is the signaling molecule of one of the most versatile and evolutionarily conserved signaling pathways. cAMP is produced by adenylyl cyclase enzymes that use ATP...
J Exp Biol (2012) 215 (18): 3242–3253.
Published: 15 September 2012
... in body ammonia levels and to a downregulation of V-ATPase (subunit A) and Rh-protein mRNA. Further, a 2 day exposure to non-lethal ammonia concentrations (1 mmol l −1 ) caused a doubling of body ammonia levels and led to an increase in Rh-protein and Na + /K + -ATPase (α-subunit) mRNA expression levels...
J Exp Biol (2012) 215 (8): 1337–1345.
Published: 15 April 2012
...Bettina Schewe; Wolfgang Blenau; Bernd Walz SUMMARY Salivary gland cells of the blowfly Calliphora vicina have a vacuolar-type H + -ATPase (V-ATPase) that lies in their apical membrane and energizes the secretion of a KCl-rich primary saliva upon stimulation with serotonin (5-hydroxytryptamine...
J Exp Biol (2010) 213 (22): 3894–3905.
Published: 15 November 2010
... + /K + -ATPase activity, and expression of gill Na + /K + -ATPase α-subunit and V-ATPase B subunit mRNA during salinity (S) acclimation. We also characterize V-ATPase kinetics and the organization of transport-related membrane systems in the gill epithelium. Macrobrachium amazonicum strongly hyper...
J Exp Biol (2010) 213 (2): 295–300.
Published: 15 January 2010
... unknown and needs to be investigated. * Author for correspondence ( firstname.lastname@example.org ) † Present address: Department of Biological Sciences, Wagner College, Staten Island, NY 10301, USA 12 10 2009 © 2010. V-ATPase acid secretion acid–base balance mosquito posterior midgut...
J Exp Biol (2009) 212 (11): 1604–1610.
Published: 1 June 2009
...Shai Saroussi; Nathan Nelson SUMMARY The life of every eukaryotic cell depends on the function of vacuolar H + -ATPase (V-ATPase). Today we know that V-ATPase is vital for many more physiological and biochemical processes than it was expected three decades ago when the enzyme was discovered...
J Exp Biol (2009) 212 (11): 1611–1619.
Published: 1 June 2009
... it appears to be established that the cation pump is a two-component system of a H + -transporting V-ATPase and a cation/nH + antiporter. After tracing the discovery of the V-ATPase as the energizer of K + /nH + antiport in the larval midgut of the tobacco hornworm Manduca sexta we show that research...
J Exp Biol (2009) 212 (3): 341–346.
Published: 1 February 2009
...Markus Huss; Helmut Wieczorek SUMMARY V-ATPases constitute a ubiquitous family of heteromultimeric, proton translocating proteins. According to their localization in a multitude of eukaryotic endomembranes and plasma membranes, they energize many different transport processes. Currently, a handful...
J Exp Biol (2008) 211 (20): 3306–3314.
Published: 15 October 2008
...Khaled H. Ahmed; Bernd Pelster SUMMARY Exposure of trout hepatocytes to hypertonicity induced a decrease in acridine orange (AO) fluorescence, indicating a corresponding decrease in pH inside the lumen of acidic compartments (pH L ). Pre-exposure of cells to the specific V-ATPase inhibitor...
J Exp Biol (2008) 211 (19): 3067–3076.
Published: 1 October 2008
... published data exist on the recta of anopheline larvae. To gain insight into the structure and functions of this organ in anopheline species, we used immunohistochemistry to compare the localization of three proteins [carbonic anhydrase (CA9),Na + /K + P-ATPase and H + V-ATPase] in the recta of anopheline...
J Exp Biol (2008) 211 (7): 1102–1108.
Published: 1 April 2008
...Jian Zuo; Sandra Vergara; Shinya Kohno; L. Shannon Holliday SUMMARY Vacuolar H + -ATPase (V-ATPase) is a fundamentally important enzyme in eukaryotic cells that is responsible for acidification of endocytic compartments. The B subunits of V-ATPases from mammals and tobacco hornworm have been shown...
J Exp Biol (2008) 211 (5): 805–815.
Published: 1 March 2008
...Bettina Schewe; Elmar Schmälzlin; Bernd Walz SUMMARY Blowfly salivary gland cells have a vacuolar-type H + -ATPase(V-ATPase) in their apical membrane that energizes secretion of a KCl-rich saliva upon stimulation with serotonin (5-hydroxytryptamine, 5-HT). We have used BCECF to study...
Kenneth M. Sterling, Prabir K. Mandal, Barbara A. Roggenbeck, Sean E. Ahearn, George A. Gerencser, Gregory A. Ahearn
J Exp Biol (2007) 210 (19): 3484–3493.
Published: 1 October 2007
... vesicles (LMV) occurred by a vanadate- and thapsigargin-sensitive ATPase that was stimulated by a transmembrane proton gradient established by a co-localized V-ATPase associated with this organelle. In the present study, hepatopancreatic LMV from the American lobster Homarus americanus were prepared...
J Exp Biol (2007) 210 (8): 1463–1471.
Published: 15 April 2007
...Carsten Hille; Bernd Walz SUMMARY Cells of the dopaminergically innervated salivary ducts in the cockroach Periplaneta americana have a vacuolar-type H + -ATPase(V-ATPase) of unknown function in their apical membrane. We have studied whether dopamine affects intracellular pH (pH i ) in duct cells...