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Keywords: Transgenesis
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Journal Articles
J Exp Biol (2020) 223 (Suppl_1): jeb206482.
Published: 1 February 2020
... changing as a result of recent advances in the large-scale sequencing of nematode genomes and the development of new techniques for their genetic manipulation. Notably, skin-penetrating gastrointestinal nematodes in the genus Strongyloides are now amenable to transgenesis, RNAi and CRISPR/Cas9-mediated...
Journal Articles
J Exp Biol (2020) 223 (Suppl_1): jeb218198.
Published: 1 February 2020
... of interest. If the goal is to have heritable changes, the reagents must be introduced into germline cells. There are a number of routes to this end. Insect transgenesis is traditionally performed by microinjection of DNA, RNA and/or recombinant protein into developing syncytial embryos. Germline...
Journal Articles
J Exp Biol (2018) 221 (13): jeb173146.
Published: 4 July 2018
... with evidence of a constitutive inflammatory state. GH and IGF system gene expression was strongly altered by GH transgenesis and fast growth, both for baseline expression and responses to immune stimulation. Thus, GH transgenesis strongly disrupts muscle immune status and normal GH and IGF system expression...
Includes: Supplementary data
Journal Articles
J Exp Biol (2015) 218 (8): 1137–1142.
Published: 15 April 2015
... stem cell activation . EMBO J.   33 , 2782 - 2797 . 10.15252/embj.201488278 Thermes , V. , Grabher , C. , Ristoratore , F. , Bourrat , F. , Choulika , A. , Wittbrodt , J. and Joly , J.-S. ( 2002 ). I-SceI meganuclease mediates highly efficient transgenesis...
Journal Articles
J Exp Biol (2014) 217 (19): 3392–3395.
Published: 1 October 2014
...://creativecommons.org/licenses/by/3.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Growth Myotube formation Transgenesis Optimal fibre size hypothesis Growth hormone (GH) has pervasive effects on growth rate...
Includes: Supplementary data
Journal Articles
J Exp Biol (2003) 206 (21): 3835–3843.
Published: 1 November 2003
... techniques will help to compensate for these deficiencies and aid in identification of critical genes in important regulatory pathways. The study of mosquito innate immunity is one example and described here. In this study, we combine mosquito transgenesis with reverse genetics. The advantage of transgenesis...