... changing as a result of recent advances in the large-scale sequencing of nematode genomes and the development of new techniques for their genetic manipulation. Notably, skin-penetrating gastrointestinal nematodes in the genus Strongyloides are now amenable to transgenesis, RNAi and CRISPR/Cas9-mediated...

... of interest. If the goal is to have heritable changes, the reagents must be introduced into germline cells. There are a number of routes to this end. Insect transgenesis is traditionally performed by microinjection of DNA, RNA and/or recombinant protein into developing syncytial embryos. Germline...

... with evidence of a constitutive inflammatory state. GH and IGF system gene expression was strongly altered by GH transgenesis and fast growth, both for baseline expression and responses to immune stimulation. Thus, GH transgenesis strongly disrupts muscle immune status and normal GH and IGF system expression...

... stem cell activation . EMBO J.   33 , 2782 - 2797 . 10.15252/embj.201488278 Thermes , V. , Grabher , C. , Ristoratore , F. , Bourrat , F. , Choulika , A. , Wittbrodt , J. and Joly , J.-S. ( 2002 ). I-SceI meganuclease mediates highly efficient transgenesis...

...://creativecommons.org/licenses/by/3.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Growth Myotube formation Transgenesis Optimal fibre size hypothesis Growth hormone (GH) has pervasive effects on growth rate...

... techniques will help to compensate for these deficiencies and aid in identification of critical genes in important regulatory pathways. The study of mosquito innate immunity is one example and described here. In this study, we combine mosquito transgenesis with reverse genetics. The advantage of transgenesis...