... to bind actin filaments. Actin-binding activity by the B subunit is required for targeting V-ATPases to the plasma membrane of osteoclasts. Bacterially expressed B subunit from the yeast Saccharomyces cerevisiae bound actin filaments with a K d of 195 nmol l –1 . The actin-binding domain of the B subunit...

...Natalie Perzov; Vered Padler-Karavani; Hannah Nelson; Nathan Nelson SUMMARY Subunit a of V-ATPase in the yeast Saccharomyces cerevisiae , in contrast to its other subunits, is encoded by two genes VPH1 and STV1 . While disruption of any other gene encoding the V-ATPase subunits results in growth...

... . 10.1016/S0006-3495(96)79604-2 Dix , D. R. , Bridgham , J. T. , Broderius , M. A. , Byersdorfer , C. A. and Eide , D. J. ( 1994 ). The FET4 gene encodes the low affinity Fe(II) transport protein of Saccharomyces cerevisiae . J. Biol. Chem. 269 , 26092 – 26099...

... – 10426 . 10.1016/S0021-9258(18)34035-3 Ambesi , A. , Allen , K. E. and Slayman , C. W. ( 1997 ). Isolation of transport-competent secretory vesicles from Saccharomyces cerevisiae . Analyt. Biochem . 251 , 127 – 129 . 10.1006/abio.1997.2257 Ambesi , A. , Pan , R...

... the secretory pathway, also affect the targeting of V o subunits ( Bryant et al., 1998 ). The proton-translocating ATPase (H + -ATPase) found on the vacuolar membrane of the yeast Saccharomyces cerevisiae is one of the most extensively characterized members of the V-type ATPase family (V-ATPase). V...

... encoding V-ATPase subunits of Saccharomyces cerevisiae result in a phenotype that is unable to grow at high pH and is sensitive to high and low metal-ion concentrations. Treatment of these null mutants with ethyl methanesulphonate causes mutations that suppress the V-ATPase null phenotype, and these cells...

... between the V 1 and V o sectors is essential for ATP-driven proton transport, and this interaction is manipulated in vivo as a means of regulating V-ATPase activity. When yeast ( Saccharomyces cerevisiae ) cells are deprived of glucose for as little as 5 min, up to 75 % of the assembled V-ATPase complexes...

... is maintained for reasons other than respiration. * Author for correspondence (e-mail: poyton@spot.colorado.edu ) 11 3 1998 12 5 1998 © The Company of Biologists Limited 1998 mitochondria cytochrome c oxidase assembly respiration yeast Saccharomyces cerevisiae...

.... , Ohya , Y. and Iida , H. ( 1991 ). Cell cycle control by calcium and calmodulin in Saccharomyces cerevisiae . Biochim. biophys. Acta 1093 , 169 – 177 . Antebi , A. and Fink , G. R. ( 1992 ). The yeast Ca2+-ATPase homologue, PMR1, is required for normal Golgi function...

... that are indispensable for expression of vacuolar ATPase activity have been identified in the yeast Saccharomyces cerevisiae. VMA1, VMA2, VMA3, VMA5 and VMA6 were cloned and characterized on the basis of partial amino acid sequences determined with the purified subunits. Genetic and biochemical studies of the yeast Pet...

...Daniel J. Klionsky; Hannah Nelson; Nathan Nelson; Debbie S. Yaver ABSTRACT The vacuolar ATPase of the yeast Saccharomyces cerevisiae acidifies the vacuolar lumen and generates an electrochemical gradient across the vacuole membrane. We have investigated the role of compartment acidification...