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Keywords: SIET
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Journal Articles
J Exp Biol (2019) 222 (13): jeb200964.
Published: 1 July 2019
... the mechanistic details of renal acid–base regulation in C. auratus and teleost fishes in general. The present study applied the scanning ion-selective electrode technique (SIET) to measure proton flux in proximal, distal and connecting tubules of goldfish. We detected increased H + efflux into the extracellular...
Journal Articles
J Exp Biol (2018) 221 (6): jeb174904.
Published: 19 March 2018
... technique (SIET) was used to measure K + flux across the midgut and hindgut epithelium, as previously described ( Rheault and O'Donnell, 2001 ; Jonusaite et al., 2013 ; Andersen et al., 2017b ). Briefly, a K + -selective microelectrode was prepared as described above and mounted onto a headstage...
Includes: Supplementary data
Journal Articles
J Exp Biol (2017) 220 (22): 4261–4269.
Published: 15 November 2017
... electrode technique (SIET) at benign (23°C) and low (6°C) temperatures to examine K + flux across the Malpighian tubules and the rectal pads in the hindgut in five Drosophila species that differ in cold tolerance. We found that chill-tolerant species were better at maintaining K + secretion and suppressing...
Includes: Supplementary data
Journal Articles
J Exp Biol (2017) 220 (4): 588–596.
Published: 15 February 2017
... , C. M. , Wood , C. M. and O'Donnell , M. J. ( 2010 ). Functional characterization of Rhesus glycoproteins from an ammoniotelic teleost, the rainbow trout, using oocyte expression and SIET analysis . J. Exp. Biol.   213 , 1049 - 1059 . 10.1242/jeb.038752 Nguyen , H...
Journal Articles
J Exp Biol (2015) 218 (20): 3206–3214.
Published: 1 October 2015
... the rectal complex are bathed in fluid within a functional compartment, the perinephric space, which is separate from the haemolymph. In this study, the scanning ion-selective electrode technique (SIET) was used to measure transport of Na + and K + across the rectal complex and across multiple regions...
Journal Articles
J Exp Biol (2009) 212 (11): 1731–1744.
Published: 1 June 2009
...-ATPase carbonic anhydrase ion-selective microelectrodes SIET H + gradients surface pH The insect gut carries out some of the most vital functions of nutrition and solute and water balance of the organism. It is the first line of defence against ingested pathogens and also the portal...