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Keywords: SIET
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Journal Articles
J Exp Biol (2022) 225 (20): jeb244279.
Published: 17 October 2022
... Editor's choice: Functional characterization of two novel ammonia transporters (Hiat1a/b) in fish: Hiat1 is critical for development and likely involved general ammonia handling. Morpholino SIET In situ hybridization Zebrafish larvae Natural Sciences and Engineering Research Council...
Includes: Supplementary data
Journal Articles
J Exp Biol (2019) 222 (13): jeb200964.
Published: 1 July 2019
... the mechanistic details of renal acid–base regulation in C. auratus and teleost fishes in general. The present study applied the scanning ion-selective electrode technique (SIET) to measure proton flux in proximal, distal and connecting tubules of goldfish. We detected increased H + efflux into the extracellular...
Journal Articles
J Exp Biol (2018) 221 (6): jeb174904.
Published: 19 March 2018
... exposure of insects to low temperatures can mitigate cold-induced ion imbalances and improve chill tolerance. Plasticity of ion and fluid transport across the Malpighian tubule and rectal epithelia probably drive this response. Chill tolerance Ionoregulation Gut Malpighian tubules SIET...
Includes: Supplementary data
Journal Articles
J Exp Biol (2017) 220 (22): 4261–4269.
Published: 15 November 2017
... electrode technique (SIET) at benign (23°C) and low (6°C) temperatures to examine K + flux across the Malpighian tubules and the rectal pads in the hindgut in five Drosophila species that differ in cold tolerance. We found that chill-tolerant species were better at maintaining K + secretion and suppressing...
Includes: Supplementary data
Journal Articles
J Exp Biol (2017) 220 (4): 588–596.
Published: 15 February 2017
... between control and experimental groups for AeRh50 monomer abundance following dsRNA treatment. Two-tailed t -tests were used to determine significance between control and experimental groups for all other parameters. For SIET data, a single biological replicate is defined as the average flux of five...
Journal Articles
J Exp Biol (2015) 218 (20): 3206–3214.
Published: 1 October 2015
... the rectal complex are bathed in fluid within a functional compartment, the perinephric space, which is separate from the haemolymph. In this study, the scanning ion-selective electrode technique (SIET) was used to measure transport of Na + and K + across the rectal complex and across multiple regions...
Journal Articles
J Exp Biol (2009) 212 (11): 1731–1744.
Published: 1 June 2009
.... * Author for correspondence (e-mail: tripathi@tifr.res.in ) 16 3 2009 2009 stereology microperfusion H + V-ATPase carbonic anhydrase ion-selective microelectrodes SIET H + gradients surface pH The insect gut carries out some of the most vital functions of nutrition...