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Keywords: RNAi
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Journal Articles
J Exp Biol jeb.244063.
Published: 17 May 2022
... life stages, despite a potentially conserved Cellular Stress Response. We used RNAseq to compare transcript expression in response to a cold stressor in Drosophila melanogaster larvae and adults and used RNAi (RNA interference) to test whether knocking down nine target genes differentially affected...
Journal Articles
J Exp Biol (2021) 224 (8): jeb238816.
Published: 16 April 2021
... flagellomeres appear rich in trichoid and grooved sensilla, known to be associated with olfactory functions. Through an RNAi approach, topically delivered HhTAR1 dsRNA induced a 50% downregulation in gene expression after 24 h in H. halys 2nd instar nymphs. An innovative behavioural assay revealed that HhTAR1...
Includes: Supplementary data
Journal Articles
J Exp Biol (2020) 223 (13): jeb223529.
Published: 8 July 2020
..., novel miR-26, which inhibits translation of the phosphofructokinase (PFK) gene by targeting the 3′ untranslated region (UTR) of pfk directly, thereby inhibiting molting and body length growth of the freshwater shrimp Neocaridina heteropoda . Lowering expression of pfk by RNA interference (RNAi) led...
Includes: Supplementary data
Journal Articles
J Exp Biol (2020) 223 (3): jeb208611.
Published: 5 February 2020
... in these experiments, we identified some genes with differential expression following a decrease of HSF-1. More complete knockdown of HSF would likely expand this list. Brunquell et al. (2016) used RNAi feeding with larval C. elegans , which appears to be an effective method of RNAi delivery in that system...
Includes: Supplementary data
Journal Articles
J Exp Biol (2020) 223 (Suppl_1): jeb206482.
Published: 1 February 2020
... changing as a result of recent advances in the large-scale sequencing of nematode genomes and the development of new techniques for their genetic manipulation. Notably, skin-penetrating gastrointestinal nematodes in the genus Strongyloides are now amenable to transgenesis, RNAi and CRISPR/Cas9-mediated...
Journal Articles
J Exp Biol (2019) 222 (21): jeb203455.
Published: 6 November 2019
... into the hemolymph. Lipophorin returns to the high-density form HDLp, which remains in the hemolymph and can resume transporting DAG from the fat body to the flight muscle ( Van der Horst and Rodenburg, 2010 ). Following the completed flight experiment, hemolymph was collected from both control and RNAi-treated...
Journal Articles
J Exp Biol (2019) 222 (7): jeb175737.
Published: 4 April 2019
...Alex M. Zimmer; Yihang K. Pan; Theanuga Chandrapalan; Raymond W. M. Kwong; Steve F. Perry ABSTRACT Loss-of-function technologies, such as morpholino- and RNAi-mediated gene knockdown, and TALEN- and CRISPR/Cas9-mediated gene knockout, are widely used to investigate gene function and its...
Journal Articles
J Exp Biol (2018) 221 (19): jeb184606.
Published: 5 October 2018
...Cristina Rodríguez-García; Jay D. Evans; Wenfeng Li; Belén Branchiccela; Jiang Hong Li; Matthew C. Heerman; Olubukola Banmeke; Yazhou Zhao; Michele Hamilton; Mariano Higes; Raquel Martín-Hernández; Yan Ping Chen ABSTRACT RNA interference (RNAi) is a post-transcriptional gene silencing mechanism...
Journal Articles
J Exp Biol (2017) 220 (8): 1400–1404.
Published: 15 April 2017
... not show eGFP fluorescence, but could still express shRNAs. RNAi-based strategies frequently use viral vectors for specific manipulation of gene expression at defined tissue sites in the rodent brain. Previous studies have employed shRNA constructs without apparent detrimental effects on cell...
Includes: Supplementary data
Journal Articles
J Exp Biol (2015) 218 (21): 3487–3498.
Published: 1 November 2015
... and 218 nm. As the expression of Cq-M15 was significantly increased in relevant molt stages and locations related to exoskeleton and teeth assembly, a study of Cq-M15 function in vivo through an RNAi loss of function approach was attempted by Cq-M15 dsRNA administration. First, the Cq-M15...
Includes: Supplementary data
Journal Articles
J Exp Biol (2015) 218 (1): 41–49.
Published: 1 January 2015
... (RNAi) was first described in C. elegans ( Fire et al., 1998 ) as a gene silencing process produced by degradation of targeted mRNA by the introduction of a short, double-stranded RNA (dsRNA) from that gene. In the first description of RNAi, Fire and colleagues determined three basic characteristics...
Journal Articles
J Exp Biol (2014) 217 (10): 1653–1655.
Published: 15 May 2014
... that regulates male sex determination in XY beetles are not known. Parental RNAi-aided knockdown in expression of tra masculinizes genetic females (XX) that are fertile. The virgin females mated with these masculinized genetic females produced all female progeny. We present the genetic evidence to show...
Journal Articles
J Exp Biol (2013) 216 (19): 3724–3732.
Published: 1 October 2013
... and studied in the forager stage of workers. Thus, we exploited the well-established RNA interference (RNAi) protocol for Vg knockdown to investigate its downstream effects on microRNA population in honey bee foragers' brain and fat body tissue. To identify microRNAs that are differentially expressed between...
Includes: Supplementary data
Journal Articles
J Exp Biol (2012) 215 (15): 2601–2610.
Published: 1 August 2012
... multidrug resistance-associated protein (dMRP, CG6214 ), a multidrug efflux transporter (MET, CG30344 ), and an organic anion transporting polypeptide 58Dc (OATP58Dc, CG3380 ). RNA interference (RNAi) knockdown and P-element insertion mutation of single OA transporter genes were used to evaluate...
Journal Articles
J Exp Biol (2011) 214 (16): 2671–2677.
Published: 15 August 2011
... 2011. 2011 Penaeus monodon crustacean myostatin/GDF11 growth RNAi Mean daily gain (MDG; g d –1 ) [(final mass–initial mass)/growing period] and hepatosomatic index (HSI) [(hepatopancreas mass/body mass)×100] were calculated at the end of the experiment. Tanks received 1.6 l...
Journal Articles
J Exp Biol (2010) 213 (24): 4146–4150.
Published: 15 December 2010
... thermal conditions. * Author for correspondence ( herve.colinet@uclouvain.be ) 20 9 2010 © 2010. 2010 Drosophila Hsp22 Hsp23 recovery chill coma RNAi Insects have evolved a range of molecular adaptations to cope with seasonal exposure to stressful (high...
Journal Articles
J Exp Biol (2005) 208 (13): 2497–2502.
Published: 1 July 2005
... lamina's major components, has previously been shown to bind several surface proteins of Plasmodium ookinetes. Here, using the recently developed RNAi technique in mosquitoes, we used a specific dsRNA construct targeted against the LANB2 gene (laminin γ1) of Anopheles gambiae to reduce its mRNA levels...
Journal Articles
J Exp Biol (2003) 206 (21): 3835–3843.
Published: 1 November 2003
... sequences. However, the lack of a complete genetic tool box for mosquitoes remains a serious obstacle in our ability to study essential mosquito-specific mechanisms. Unlike Drosophila , very few null mutations for mosquito genes exist. The development of reverse-genetic analyses based on RNAi and transgenic...
Journal Articles
J Exp Biol (2003) 206 (11): 1817–1823.
Published: 1 June 2003
...Anthony E. Brown; Andrea Crisanti; Flaminia Catteruccia SUMMARY Heritable RNA interference (RNAi) mediated by transgenes exhibiting dyad symmetry represents an important tool to study the function of genes expressed at late developmental stages. In this study, we determined whether...