... across nine thermally distinct Fundulus heteroclitus populations . These traits included whole-animal metabolism (WAM), critical thermal maximum (CT max ) and substrate-specific cardiac metabolism measured in approximately 350 individuals. These traits exhibited high variation among both individuals...

...Chris M. Wood; Ilan M. Ruhr; Kevin L. Schauer; Yadong Wang; Edward M. Mager; M. Danielle McDonald; Bruce Stanton; Martin Grosell ABSTRACT Freshwater- and seawater-acclimated Fundulus heteroclitus were exposed to acute hypoxia (10% air saturation, 3 h), followed by normoxic recovery (3 h). In both...

... acclimation and putative local thermal adaptation on mitochondrial capacity, proton leak and P/O ratios in two subspecies of Atlantic killifish ( Fundulus heteroclitus ). Ucp1 was the dominant isoform in liver and was more highly expressed in northern killifish. We found that cold acclimation increased...

... is modified to allow Na + secretion in hypersaline environments. Mummichogs ( Fundulus heteroclitus ) acclimated to hypersaline [2× seawater (2SW), 64‰] for 30 days developed invasive projections of accessory cells with an increased area of tight junctions, detected by punctate distribution of CFTR (cystic...

...Reid S. Brennan; Fernando Galvez; Andrew Whitehead ABSTRACT The killifish Fundulus heteroclitus is an estuarine species with broad physiological plasticity, enabling acclimation to diverse stressors. Previous work suggests that freshwater populations expanded their physiology to accommodate low...

... of animals, while behaviour determines which environments are encountered. Here, we identified changes in the behavioural selection of external salinity in Fundulus heteroclitus , an estuarine teleost, as a consequence of digesting a meal. Fish that consumed high levels of dietary calcium exhibited a higher...

...Andrew Whitehead; Jennifer L. Roach; Shujun Zhang; Fernando Galvez SUMMARY The killifish Fundulus heteroclitus is abundant in osmotically dynamic estuaries and it can quickly adjust to extremes in environmental salinity. We performed a comparative osmotic challenge experiment to track...

...Graham R. Scott; Daniel W. Baker; Patricia M. Schulte; Chris M. Wood SUMMARY We have explored the molecular and physiological responses of the euryhaline killifish Fundulus heteroclitus to transfer from brackish water (10% seawater) to 100% seawater for 12 h, 3 days or 7 days. Plasma[Na + ] and [Cl...

...Kelly A. Hyndman; David H. Evans SUMMARY In euryhaline fishes like the killifish ( Fundulus heteroclitus )that experience daily fluctuations in environmental salinity, endothelin 1(EDN1) may be an important regulator molecule necessary to maintain ion homeostasis. The purpose of this study...

...L. López-Bojórquez; P. Villalobos; C. García-G.; A. Orozco; C. Valverde-R. SUMMARY The physiological role played by thyroid hormones (TH) in hydro-osmotic homeostasis in fish remains a controversial issue. Previous studies have shown that in Fundulus heteroclitus (killifish) hypo-osmotic stress...

...Douglas L. Crawford; Marjorie F. Oleksiak SUMMARY Functional genomics research using Fundulus heteroclitus has focused on variation among individuals because of the evolutionary importance and value of Fundulus in explaining the human condition (why individual humans are different and are affected...

...Graham R. Scott; Patricia M. Schulte; Chris M. Wood SUMMARY We have explored intestinal function in the euryhaline killifish Fundulus heteroclitus after transfer from brackish water (10%seawater) to fresh water. Plasma Na + and Cl – concentrations fell at 12 h post-transfer, but recovered by 7 days...

.... Degenerate primers, reverse transcription–PCR and rapid amplification of cDNA ends were used to deduce the complete cDNA sequence of a putative COX2 enzyme from the gills of the euryhaline killifish ( Fundulus heteroclitus ). The 2738 base pair cDNA includes a coding region for a 610 amino acid protein...

...Graham R. Scott; James B. Claiborne; Susan L. Edwards; Patricia M. Schulte; Chris M. Wood SUMMARY We have explored the molecular basis for differences in physiological function between the gills and opercular epithelium of the euryhaline killifish Fundulus heteroclitus . These tissues...

... in the in vitro opercular skin of a teleost ( Fundulus heteroclitus ), a gill-like epithelium rich in chloride cells. J. Physiol. Lond. 271 , 155 -191. Dowd, B. F. X. and Forbush, B. ( 2002 ). PASK(proline-alanine-rich STE20-related kinase), a regulatory kinase of the Na-K-Cl cotransporter (NKCC1). J...

...Raquel N. Lima; Dietmar Kültz SUMMARY The effects of salinity on chloride cells (CC) and Na + /K + -ATPase content in gill epithelium of euryhaline killifish Fundulus heteroclitus were analyzed using laser scanning cytometry (LSC) and tissue microarrays (TMAs). Salinity acclimations consisted...

... in Fundulus heteroclitus . J. Exp. Biol. 204 , 1889 -1899. Greco, A. M., Fenwick, J. C. and Perry, S. F. ( 1996 ). The effects of soft-water acclimation on gill structure in the rainbow trout Oncorhynchus mykiss . Cell Tissue Res. 285 , 75 -82. Hirai, N., Tagawa, M., Kaneko, T., Seikai, T...

...Fumi Katoh; Susumu Hyodo; Toyoji Kaneko SUMMARY We examined the involvement of mitochondria-rich (MR) cells in ion uptake through gill epithelia in freshwater-adapted killifish Fundulus heteroclitus , by morphological observation of MR cells and molecular identification of the vacuolar-type proton...

... transmembrane conductanctance regulator protein trafficking Na + ,K + ,2Cl - cotransporter osmoregulation euryhaline teleost killifish Fundulus heteroclitus Mitochondria-rich chloride (MR) cells of the skin and gill epithelia of teleost fish are responsible for ion uptake in fresh water (FW...

...K. Daborn; R. R. F. Cozzi; W. S. Marshall SUMMARY Freshwater-adapted killifish ( Fundulus heteroclitus ) opercular epithelia were dissected and subjected to blood-side hypertonic bathing solution in Ussing-style chambers to simulate the increase in blood osmolality during migration to sea water...