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Keywords: Cryoprotectant
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Journal Articles
Journal Articles
J Exp Biol (2020) 223 (24): jeb233478.
Published: 29 December 2020
...Jessica R. Kennedy; Christopher D. G. Harley; Katie E. Marshall ABSTRACT Freezing is an extreme stress to living cells, and so freeze-tolerant animals often accumulate protective molecules (termed cryoprotectants) to prevent the cellular damage caused by freezing. The bay mussel, Mytilus trossulus...
Includes: Supplementary data
Journal Articles
Journal Articles
J Exp Biol (2016) 219 (15): 2358–2367.
Published: 1 August 2016
... Metabolomics Cryoprotectant Compatible solute Preferential exclusion Protein aggregation Organisms as diverse as bacteria, plants and animals are known to naturally accumulate small protective molecules in response to various environmental stressors (recently reviewed in Yancey and Siebenaller...
Includes: Supplementary data
Journal Articles
Journal Articles
J Exp Biol (2014) 217 (12): 2193–2200.
Published: 15 June 2014
..., recording microclimate, and determining frog survival in spring. We measured cryoprotectant (glucose) concentrations and identified the presence of antifreeze glycolipids in tissues from subsamples of naturally freezing frogs. We also recorded the behavior of wood frogs preparing to freeze in artificial...
Includes: Supplementary data
Journal Articles
J Exp Biol (2013) 216 (18): 3461–3473.
Published: 15 September 2013
... of freezing at −4°C. The profound freeze tolerance is presumably due to their high levels of organic osmolytes and bound water, which limits ice formation. Adaptive responses to freezing (−2.5°C for 48 h) and subsequent thawing (4°C) included synthesis of the cryoprotectants urea and glucose, and dehydration...
Journal Articles
Journal Articles
J Exp Biol (2012) 215 (3): 543–551.
Published: 1 February 2012
... of the cryoprotectant glucose into blood and all tissues. As glycogenesis is both an energy-expensive process and counter-productive to maintaining sustained high cryoprotectant levels, we proposed that glycogen synthase kinase-3 (GSK-3) would be activated when wood frogs froze and would phosphorylate its downstream...
Journal Articles
Journal Articles
J Exp Biol (2003) 206 (13): 2221–2228.
Published: 1 July 2003
...Xiang-Hong Liu; Hongjun Pan; Peter Mazur SUMMARY In this study, we applied proton NMR to measure the permeation of two cryoprotective agents (CPAs), ethylene glycol (EG) and methanol, into 1st instar Anopheles larvae. Calibration with standard solutions of EG or methanol (0–10 mol l -1 ) confirmed...
Journal Articles
J Exp Biol (2001) 204 (9): 1659–1666.
Published: 1 May 2001
... the cooling phase of an ecologically based thermoperiod, (ii) whether the protection afforded was lost during warming or contributed to increased cold-tolerance during subsequent cycles and (iii) whether the major thermally inducible stress protein (Hsp70) or carbohydrate cryoprotectants contributed...