... the Bohr effect, we took replicate measurements of stripped isoHbs under identical conditions at pH 6.7 and 7.4. The final pH of Hb solutions used in each experiment was measured at 25°C using an InLab Micro pH electrode (Mettler Toledo). We measured O 2 equilibria of 3 µl thin-film samples in a modified...

... as Hb–O 2 binding affinity and sensitivity to pH (Bohr effect), CO 2 , temperature and 2,3-diphosphoglycerate (DPG, the major allosteric modulator of Hb–O 2 affinity in red blood cells) in four social and two solitary species of African mole rats (family Bathyergidae) originating from different biomes...

... explanation for the phenomenon may involve at least two not necessarily exclusive factors. First, the large Bohr effect suggests an above average increase in the number of proton binding sites in deoxyHb compared with oxyHb (for review, see Berenbrink, 2006 , 2011a ). The release of these protons during...

... reverse Bohr effect and marked cooperativity. Dialysis increased Hc-O 2 affinity, obliterated cooperativity and decreased the pH-sensitivity of O 2 binding. Replacing Mg 2+ and Ca 2+ restored the native O 2 -binding properties and the reverse Bohr shift. l - and d -lactate exerted minor modulatory effects...

... Bohr effect Haldane effect Na + /H + exchange anion exchange intracellular pH oxygen equilibrium curve agnathan fish The amount of oxygen transported per unit volume of blood depends on the oxygen tension, on the number of red cells, on the amount of haemoglobin within the cell...

... of the extracellular compartment. As a consequence, extracellular acid loads cause marked fluctuations in plasma pH. 14 09 1995 © The Company of Biologists Limited 1995 oxygen transport carbon dioxide transport Haldane effect Bohr effect Agnatha lamprey erythrocyte haemoglobin acid–base...

... vertebrates. When the red blood cell pH is used to determine the Bohr effect, it is apparent that CO 2 transport may take precedence over oxygen delivery in the blood of these animals. The present in vitro results also confirm that the majority of CO 2 added to the blood by the tissues is probably...

... of the resulting oxygen isobars (ΔHcyO 2 /ΔpH or ΔS/ΔpH) (where S is oxygen saturation) depend on pH. The diagram allows evaluation of both the Bohr coefficients (ΔlogP 50 /ΔpH) and the Hill coefficients (riso) at specific pH values. It provides an integrated illustration of the importance of the Bohr effect...

... of California, San Diego, La Jolla, CA, USA. † Present address: Nomo Fisheries Station, Nagasaki University, Nomozaki, Nagasaki 851-05, Japan. 25 03 1987 © 1987 by Company of Biologists 1987 Bohr effect blood buffer value CO 2 dissociation curves Haldane effect oxygen affinity...