...Jinae N. Roa; Martin Tresguerres ABSTRACT Na + /K + -ATPase (NKA)- and vacuolar H + -ATPase (VHA)-rich cells in shark gills secrete excess acid and base, respectively, to seawater to maintain blood acid–base homeostasis. Both cell types are rich in mitochondria, indicating high ATP demand; however...

...Keren Aviezer-Hagai; Vered Padler-Karavani; Nathan Nelson SUMMARY V-ATPase null mutants in yeast have a distinct, conditionally lethal phenotype that can be obtained through disruption of any one of its subunits. This enables supplementation of this mutant with the relevant subunit tagged...

... the temperature interactions of a major cytosolic molecular chaperone, Hsc70, from the eurythermal marine goby Gillichthys mirabilis . Using in vitro assays, we measured the intrinsic activity, unfolded-protein-stimulated activity, temperature sensitivity and heat stability of the ATPase activity of native Hsc70...

..., ATP, which is the substrate of dynein ATPase and the actual energy source for ciliary beating, does not need to diffuse into the cilia. Hydrolyzed ATP must be immediately recycled by ciliary arginine kinase to be rephosphorylated by phosphoarginine. Thus, the intraciliary concentration of ATP...

... and exocytosis. Their lumina are acidic, with pH ranging from 4.5 to 6.5. This article describes recent studies on these animal cell organelles focusing on (1) the primary proton pump (vacuolar-type H + -ATPase) and (2) the functions of the organelle luminal acidity. We also discuss similarities and differences...

... + /K + pump activity. * Author for correspondence (e-mail: jsanchez@porthos.bio.ub.es ). 2 8 1996 ©The Company of Biologists Limited 1996 ASC system ATPase Na + /K + pump glucagon adaptive regulation fasting hepatocytes trout Salmo trutta System...

...Pablo J. Schwarzbaum; Ramón O. Bernabeu; Gerhard Krumschnabel; Christina Biasi; Wolfgang Wieser ABSTRACT Protein kinase C (PKC) and Na + /K + -ATPase in hepatocytes from the anoxia-tolerant goldfish ( Carassius auratus ) and the anoxia-intolerant rainbow trout ( Oncorhynchus mykiss ) were studied...

...Lincoln Taiz ABSTRACT Plant cells are unique in containing large acidic vacuoles which occupy most of the cell volume. The vacuolar H + -ATPase (V-ATPase) is the enzyme responsible for acidifying the central vacuole, although it is also present on Golgi and coated vesicles. Many secondary transport...

...Patricia M. Kane ABSTRACT Achieving an understanding of the biosynthesis, assembly and intracellular targeting of the vacuolar H + -ATPase is critical for understanding the distribution of acidic compartments and the regulation of organelle acidification. The assembly of the yeast vacuolar H...

...J. Peter Gogarten; Thomas Starke; Henrik Kibak; Jenny Fishmann; Lincoln Taiz ABSTRACT The structure of V- and F-ATPases/ATP synthases is remarkably conserved throughout evolution. Sequence analyses show that the V- and F-ATPases evolved from the same enzyme that was already present in the last...

...Barry J. Bowman; William J. Dschida; Emma Jean Bowman ABSTRACT We are using three approaches to investigate the vacuolar ATPase, V-ATPase, from Neurospora crassa . (1) Examination in the electron microscope shows the enzyme has a ‘ball and stalk’ structure like the F-type ATPases. However...

... the strong net uptakes of Na + and Cl − to be similar at approximately 4.5 μ molh −1 g −1 from full-strength urine, diluting the fluid to a threshold below 20 mmol l −1 . Na + /K + -ATPase and Ca 2+ -ATPase activities of gill homogenates indicated V max values (65–127 and 11 nmol min −1 mg −1 protein...

...TA McKean 19 10 1989 © 1990 by Company of Biologists 1990 muskrat ATPase myosin heart References Darnell , J. , Lodesh , H. and Baltimore , D. ( 1986 ). Molecular Cell Biology . New York : Scientific American Books , pp. 627 – 629...

... been provided for primary chloride transport despite numerous reports of cellular, anion-stimulated ATPases and of chloride transport that cannot be accounted for by the three well-accepted chloride transport processes. Anion-stimulated ATPases are of mitochondrial origin and are a ubiquitous property...