The present study examined if 20-weeks high-fat diet (HFD) consumption had a temperature specific effect on the contractile performance and regional thermal sensitivity of isolated mouse soleus (SOL) and diaphragm (DIA) muscle. Four-week-old female CD-1 mice were randomly selected to consume either a standard laboratory diet or a standard laboratory diet in conjunction with a HFD for 20-weeks. Peripheral SOL and core DIA were isolated from each animal and maximal isometric force and work loop power were assessed at 20⁰C, 28⁰C, 35⁰C and 40⁰C. Increasing temperature to 35⁰C resulted in greater isometric stress, lower activation and relaxation time and higher work loop power in both muscles. A further increase in temperature to 40⁰C did not affect isometric force but increased work loop power output of the SOL. Conversely, isometric force of the DIA was reduced and work loop power maintained when temperature was increased to 40⁰C. HFD consumption resulted in greater isometric force and absolute work loop power of the SOL and reduced isometric stress of the DIA, effects that were less apparent at lower temperatures. When the relationship between temperature and each measure of contractile function was examined by linear regression, there was no difference in slope between the control or HFD groups for either SOL or DIA. These results indicate that whilst contractile function initially increases with temperature, the temperature to elicit maximal performance is muscle and contractile mode-specific. Furthermore, HFD effects on contractile function are temperature specific, but HFD does not influence the relationship between temperature and performance.
High-fat diet affects measures of skeletal muscle contractile performance in a temperature specific manner but does not influence regional thermal sensitivity
2023 JEB Outstanding Paper Prize shortlist and winner
The JEB Editors are delighted to announce the shortlisted authors for the 2023 JEB Outstanding Paper Prize. Read the winning paper - Tiny spies: mosquito antennae are sensitive sensors for eavesdropping on frog calls - by Hoover Pantoja-Sanchez and Brian Leavell from Ximena Bernal's lab at Purdue University, USA.
JEB Science Communication Workshop for ECRs
If you’re an early-career researcher interested in science communication and are attending the SEB Annual Conference in Prague this summer, come a day early and join the JEB Editors at a sci comm workshop to learn the key writing skills needed to promote your research to a broad audience beyond your peers (1 July at 14.30-17.30). Places are limited to 24 attendees, and applicants should apply through the SEB registration page by 30 April 2024.
Bridging the gap between controlled conditions and natural habitats in understanding behaviour
Novel technologies enable behavioural experiments with non-model species, in naturalistic habitats and with underexplored behaviours. In their Commentary, Scholz and colleagues discuss how to obtain a deeper understanding of the natural ecology and lifestyle of study animals.
Beluga metabolic measures could help save species
To help save animals from extinction, it’s important to understand what each species needs to survive. This led Jason John et al. to measure the metabolic rates of captive belugas to develop a ‘fish calculator’ showing that the whales need to eat ~23 salmon per day.
ECR Workshop on Positive Peer Review
Are you an ECR looking for tips on how to write concise, astute and useful manuscript reviews? If so, join the JEB Editors at a 2-hour JEB-sponsored Workshop on Positive Peer Review at the Canadian Society of Zoologists annual meeting in Moncton on 9 May 2024 at 13.00-15.00. There are 25 spaces for ECRs and selection is first come, first serve. To sign up, check the ECR Workshop box when you register for the CSZ meeting.
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