The equine microbiome can change in response to dietary alteration and might play a role in insulin dysregulation. The aim of this study was to determine the effect of adding pasture to a hay diet on the fecal bacterial microbiome of both healthy and insulin-dysregulated ponies. Fecal samples were collected from sixteen ponies before and after dietary change to enable bacterial 16S rRNA sequencing of the V3-V4 region. The dominant phyla in all samples were the Firmicutes and Bacteroidetes. The evenness of the bacterial populations decreased after grazing pasture, and when a pony was moderately insulin-dysregulated (P=0.001). Evenness scores negatively correlated with post-prandial glucagon-like peptide-1 concentrations after a hay-only diet (r2;=-0.7, P=0.001). A change in diet explained 3% of fecal microbiome variability. We conclude that metabolically healthy ponies have greater microbial stability when challenged with a subtle dietary change, compared to moderately insulin-dysregulated ponies.
The effect of diet change and insulin dysregulation on the fecal microbiome of ponies
Currently Viewing Accepted Manuscript - Newer Version Available
Danielle M. Fitzgerald, Robert J. Spence, Zachary K. Stewart, Peter J. Prentis, Martin N. Sillence, Melody A. de Laat; The effect of diet change and insulin dysregulation on the fecal microbiome of ponies. J Exp Biol 2020; jeb.219154. doi: https://doi.org/10.1242/jeb.219154
Download citation file:
Advertisement
2023 JEB Outstanding Paper Prize shortlist and winner
The JEB Editors are delighted to announce the shortlisted authors for the 2023 JEB Outstanding Paper Prize. Read the winning paper - Tiny spies: mosquito antennae are sensitive sensors for eavesdropping on frog calls - by Hoover Pantoja-Sanchez and Brian Leavell from Ximena Bernal's lab at Purdue University, USA.
JEB Science Communication Workshop for ECRs
If you’re an early-career researcher interested in science communication and are attending the SEB Annual Conference in Prague this summer, come a day early and join the JEB Editors at a sci comm workshop to learn the key writing skills needed to promote your research to a broad audience beyond your peers (1 July at 14.30-17.30). Places are limited to 24 attendees, and applicants should apply through the SEB registration page by 30 April 2024.
Bridging the gap between controlled conditions and natural habitats in understanding behaviour
Novel technologies enable behavioural experiments with non-model species, in naturalistic habitats and with underexplored behaviours. In their Commentary, Scholz and colleagues discuss how to obtain a deeper understanding of the natural ecology and lifestyle of study animals.
Beluga metabolic measures could help save species
To help save animals from extinction, it’s important to understand what each species needs to survive. This led Jason John et al. to measure the metabolic rates of captive belugas to develop a ‘fish calculator’ showing that the whales need to eat ~23 salmon per day.
ECR Workshop on Positive Peer Review
Are you an ECR looking for tips on how to write concise, astute and useful manuscript reviews? If so, join the JEB Editors at a 2-hour JEB-sponsored Workshop on Positive Peer Review at the Canadian Society of Zoologists annual meeting in Moncton on 9 May 2024 at 13.00-15.00. There are 25 spaces for ECRs and selection is first come, first serve. To sign up, check the ECR Workshop box when you register for the CSZ meeting.