In rainbow trout, the dominant site of Na+ uptake (JNain) and ammonia excretion (Jamm) shifts from the skin to the gills over development. Post-hatch (PH; 7 days post-hatch) larvae utilize the yolk sac skin for physiological exchange, whereas by complete yolk sac absorption (CYA; 30 days post-hatch), the gill is the dominant site. At the gills, JNain and Jamm occur via loose Na+/NH4+ exchange, but this exchange has not been examined in the skin of larval trout. Based on previous work, we hypothesized that, contrary to the gill model, JNain by the yolk sac skin of PH trout occurs independently of Jamm. Following a 12-h exposure to high environmental ammonia (HEA; 0.5 mmol l−1 NH4HCO3; [Na+]=600 µmol l−1; pH=8), Jamm by the gills of CYA trout and the yolk sac skin of PH larvae, which were isolated using divided chambers, increased significantly. However, this was coupled to an increase in JNain across the gills only, supporting our hypothesis. Moreover, gene expression of proteins involved in JNain (Na+/H+-exchanger-2 (NHE2) and H+-ATPase) increased in response to HEA only in the CYA gills. We further identified expression of the apical Rhesus (Rh) proteins Rhcg2 in putative pavement cells and Rhcg1 (co-localized with apical NHE2 and NHE3b and Na+/K+-ATPase) in putative peanut lectin agglutinin-positive (PNA+) ionocytes in gill sections. Similar Na+/K+-ATPase-positive cells expressing Rhcg1 and NHE3b, but not NHE2, were identified in the yolk sac epithelium. Overall, our findings suggest that the mechanisms of JNain and Jamm by the dominant exchange epithelium at two distinct stages of early development are fundamentally different.
Different mechanisms of Na+ uptake and ammonia excretion by the gill and yolk sac epithelium of early life stage rainbow trout
Currently Viewing Accepted Manuscript - Newer Version Available
- Split-screen
- Views Icon Views
- Open the PDF for in another window
-
Article Versions Icon
Versions
- Version of Record 01 March 2017
- Accepted Manuscript 01 January 2016
- Share Icon Share
-
Tools Icon
Tools
- Search Site
Alex M. Zimmer, Jonathan M. Wilson, Patricia A. Wright, Junya Hiroi, Chris M. Wood; Different mechanisms of Na+ uptake and ammonia excretion by the gill and yolk sac epithelium of early life stage rainbow trout. J Exp Biol 2016; jeb.148429. doi: https://doi.org/10.1242/jeb.148429
Download citation file:
Advertisement
2023 JEB Outstanding Paper Prize shortlist and winner
The JEB Editors are delighted to announce the shortlisted authors for the 2023 JEB Outstanding Paper Prize. Read the winning paper - Tiny spies: mosquito antennae are sensitive sensors for eavesdropping on frog calls - by Hoover Pantoja-Sanchez and Brian Leavell from Ximena Bernal's lab at Purdue University, USA.
JEB Science Communication Workshop for ECRs
If you’re an early-career researcher interested in science communication and are attending the SEB Annual Conference in Prague this summer, come a day early and join the JEB Editors at a sci comm workshop to learn the key writing skills needed to promote your research to a broad audience beyond your peers (1 July at 14.30-17.30). Places are limited to 24 attendees, and applicants should apply through the SEB registration page by 30 April 2024.
Bridging the gap between controlled conditions and natural habitats in understanding behaviour
Novel technologies enable behavioural experiments with non-model species, in naturalistic habitats and with underexplored behaviours. In their Commentary, Scholz and colleagues discuss how to obtain a deeper understanding of the natural ecology and lifestyle of study animals.
Beluga metabolic measures could help save species
To help save animals from extinction, it’s important to understand what each species needs to survive. This led Jason John et al. to measure the metabolic rates of captive belugas to develop a ‘fish calculator’ showing that the whales need to eat ~23 salmon per day.
ECR Workshop on Positive Peer Review
Are you an ECR looking for tips on how to write concise, astute and useful manuscript reviews? If so, join the JEB Editors at a 2-hour JEB-sponsored Workshop on Positive Peer Review at the Canadian Society of Zoologists annual meeting in Moncton on 9 May 2024 at 13.00-15.00. There are 25 spaces for ECRs and selection is first come, first serve. To sign up, check the ECR Workshop box when you register for the CSZ meeting.