Spreading depression (SD) is associated with large changes in extracellular ion concentrations and can be induced by impairing mechanisms of K+ ion homeostasis. We tested activity-dependence of SD in the locust model of ouabain-induced SD in the metathoracic ganglion. Wind-activation of thoracic circuitry resulted in small increases of K+ concentration that took 5-10 s to be cleared from the extracellular space. In the presence of the Na+/K+-ATPase inhibitor ouabain, wind stimulation every 30 s halved the latency to the first SD event and increased its duration. Wind stimulation was able to trigger the first event suggesting that local activity could determine the origin of successive SD events. Perfusion with zero-calcium saline blocked neural activity in the ganglion and prevented the occurrence of ouabain-induced SD. We conclude that ouabain-induced SD in the locust CNS is strongly dependent on the existing level of neural activity.
Activity-dependence of spreading depression in the locust CNS
Postal address (present address): Queen's University, Department of Biology, Bioscience complex, Room 3404, Kingston, Ontario, Canada K7L 3N6
Currently Viewing Accepted Manuscript - Newer Version Available
- Split-screen
- Views Icon Views
- Open the PDF for in another window
-
Article Versions Icon
Versions
- Version of Record 01 March 2016
- Accepted Manuscript 01 January 2016
- Share Icon Share
-
Tools Icon
Tools
- Search Site
Kristin E. Spong, Tom R. Mazzetti, R. Meldrum Robertson; Activity-dependence of spreading depression in the locust CNS. J Exp Biol 2016; jeb.132456. doi: https://doi.org/10.1242/jeb.132456
Download citation file:
Advertisement
2023 JEB Outstanding Paper Prize shortlist and winner
The JEB Editors are delighted to announce the shortlisted authors for the 2023 JEB Outstanding Paper Prize. Read the winning paper - Tiny spies: mosquito antennae are sensitive sensors for eavesdropping on frog calls - by Hoover Pantoja-Sanchez and Brian Leavell from Ximena Bernal's lab at Purdue University, USA.
JEB Science Communication Workshop for ECRs
If you’re an early-career researcher interested in science communication and are attending the SEB Annual Conference in Prague this summer, come a day early and join the JEB Editors at a sci comm workshop to learn the key writing skills needed to promote your research to a broad audience beyond your peers (1 July at 14.30-17.30). Places are limited to 24 attendees, and applicants should apply through the SEB registration page by 30 April 2024.
Bridging the gap between controlled conditions and natural habitats in understanding behaviour
Novel technologies enable behavioural experiments with non-model species, in naturalistic habitats and with underexplored behaviours. In their Commentary, Scholz and colleagues discuss how to obtain a deeper understanding of the natural ecology and lifestyle of study animals.
How a macrourid fish remains buoyant at depths it should be unable to reach
Fish with swimbladders should not be capable of descending below 7200m, but when Alan Jamieson and Todd Bond spotted a macrourid fish at 7259m, they knew they had seen something miraculous. Working with Imantes Priede, they reveal that the swimbladder of a 1 kg fish could hold 37.9 g of oxygen, sufficient to offset the weight of the fish's bones, and take 221-440 days to fill, which is plausible because it takes years for the fish to descend to such depths.
ECR Workshop on Positive Peer Review
Are you an ECR looking for tips on how to write concise, astute and useful manuscript reviews? If so, join the JEB Editors at a 2-hour JEB-sponsored Workshop on Positive Peer Review at the Canadian Society of Zoologists annual meeting in Moncton on 9 May 2024 at 13.00-15.00. There are 25 spaces for ECRs and selection is first come, first serve. To sign up, check the ECR Workshop box when you register for the CSZ meeting.